Induction of apoptosis by diallyl disulfide through activation of caspase-3 in human leukemia HL-60 cells11Abbreviations: Ac-DEVD-CHO, N-acetyl-Asp-Glu-Val-Asp-CHO (aldehyde); Ac-DEVD-AFC, N-acetyl-Asp-Glu-Val-Asp-AFC (7-amino-4-trifluoromethyl-coumaine); Ac-YVAD-CHO, N-acetyl-Tyr-Val-Ala-Asp-CHO (aldehyde); CAD, caspase-activated deoxyribonuclease; CM-H2DCFDA, 5-(and -6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate; DADS, diallyl disulfide; FACS, fluorescence-activated cell sorter; FITC, fluore

Kwon, Kang‐Beom; Yoo, Su-Jin; Ryu, Do-Gon; Yang, Jeong‐Yeh; Rho, Hye-Won; Kim, Jong‐Suk; Park, Jin-Woo; Kim, Hyung-Rho; Park, Byung‐Hyun

doi:10.1016/s0006-2952(01)00860-7

Cited by 135 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous studies, most investigators used low concentrations such as, 10, 25, 50 µM of DADS during short incubation periods, such as 15 minutes to 3 hours [8,10]. Only a few studies have reported the effect of DADS at 100, 200 µM and 24 or 72 hours [12,27].…”

Section: Discussionmentioning

confidence: 99%

“…To decide on the proper time to exposure to DADS, we referred to the previous studies. In some studies, ROS production after DADS treatment happened within 30 minutes, reaching peak value at roughly 2 to 3 hours [9,10]. And in the others, the anti-proliferative effect was noted after 24 or 72 hours exposed to DADS.…”

Section: Discussionmentioning

confidence: 99%

“…Anti-oxidant effects are acquired through increased activity of anti-oxidants such as glutathione peroxidase and induction of apoptosis is acquired from the mechanism associated with intracellular redox environment [7-9]. Moreover, it has been reported that DADS is able to inhibit the proliferation of several tumor cells through characteristics related to apoptosis [10-12]. But, in gastric cancer, the definite anti-cancer effect of DADS and the mechanism of its action has not been established.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Induction of apoptosis with diallyl disulfide in AGS gastric cancer cell line

Lee

Kim

et al. 2011

J Korean Surg Soc

View full text Add to dashboard Cite

PurposeDiallyl disulfide (DADS) is a major organosulfur compound derived from garlic. It has been reported that DADS is able to inhibit the proliferation of several tumor cells. In this study, the effect of DADS was investigated in terms of the proliferation of AGS, gastric adenocarcinoma cell line at various concentrations.MethodsThe viability of cultured cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. To detect the induction of apoptosis, Annexin V-FITC/propodium iodide (PI) staining assay was performed. Analysis of reactive oxygen species (ROS) and the distribution of cells in the cell cycle were measured by a flow cytometer. And using the Western blot analysis, the change of Fas, caspase-3, Bax, Bcl-2 activity was measured.ResultsThe percentage of live AGS cells was decreased to 23% of that in the control group after 400 µM DADS treatment for 48 hours. The Annexin V positive/PI negative (apoptosis portion) area increased from low concentration of DADS to high concentration. When comparing among the DADS treatment groups, the amount of ROS production increased in a dose dependent manner. The percentage of sub-diploid DNA content increased from 8.71% at 50 µM to 25.74% at 400 µM DADS treatment group. The expressions of Fas, caspase-3, Bax were increased and that of Bcl-2 was decreased in a dose dependent manner.ConclusionDADS decreases the viability of AGS cell lines and induces apoptosis in a dose-dependent manner. But the relationship of the anti-proliferative effect of DADS and related molecular changes were not clearly proportional to the concentration of DADS.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of apoptosis with diallyl disulfide in AGS gastric cancer cell line

Lee

Kim

et al. 2011

J Korean Surg Soc

View full text Add to dashboard Cite

show abstract

“…Treated samples were significantly different to the control as was assessed by the t-test (P \ 0.05), [y-axis: RLUs (relative luminescence units): factor of luminescence of ''treated/control'']. b Analysis of the cleavage of the main substrate of caspase-3 PARP-by Western blot after 4, 8, 16 and 24 h but they were rarely investigated for the treatment of human leukemia [15,17,[54][55][56][57]. In this study, we investigated a panel of synthetic diallyl-and dipropylsulfides for their potency to induce apoptosis in U937 cells used as a model.…”

Section: Discussionmentioning

confidence: 99%

Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)

et al. 2009

View full text Add to dashboard Cite

Naturally occurring organic sulfur compounds (OSCs), such as linear allylsulfides from Allium species, are attracting attention in cancer research, since several OSCs were shown to act beneficially both in chemoprevention and in chemotherapy, while hardly exerting any harmful side effects. Hence, we investigated the possible role of different OSCs in the treatment of leukemia. Thereby, we found that the compounds tested in this study induced apoptosis in U937 cells, with an efficiency depending on the number of sulfides, and selected the most promising candidate, diallyltetrasulfide (Al2S4), for detailed mechanistic studies. Here we show that Al2S4 induced an accumulation of cells in early mitosis (G2/M phase), followed by the activation of caspase-dependent apoptosis. The compound counteracted different anti-apoptotic Bcl-2 family members (Bcl-xL, phospho-Bad and Bcl-2), promoted activation of Bax and Bak and induced the release of cytochrome c into the cytoplasm. Treatment by Al2S4 let to the identification of early apoptotic events including Bcl-xL degradation, Bak activation and release of cytochrome c followed by late events including Bcl-2 proteolysis, Bax activation, Bad dephosphorylation, caspase activation, nuclear fragmentation and phosphatidylserine exposure.

show abstract

“…Nonetheless it is not always possible to make a clear cut between different ''targets'' and pathways affected, e.g. in the case of reactive oxygen species (2007), Wu et al (2005), Dashwood and Ho (2007), Herman- Antosiewicz and Singh (2004), Das et al (2007), Karmakar et al (2007), Kwon et al (2002), Filomeni et al (2003), Liu et al (2006) and Lu et al (2004) Human cancer cells ; Cytoprotective, chemopreventive Shukla and Kalra (2007), Dashwood and Ho (2007) and Chemopreventive, cytoprotective Ariga and Seki (2006), Aggarwal and Shishodia (2004), Ide and Lau (2001), Geng et al (1997), Balasenthil et al (2001Balasenthil et al ( , 2002, Ho et al (2001) (2007) Potency of OSCs in the field of cancer research was extensively shown by experimental studies in vitro and in vivo. Moreover, it was proven in several models that ROS are a key factor of the different triggered events.…”

Section: Experimental Studiesmentioning

confidence: 99%

Potential role of organic sulfur compounds from Allium species in cancer prevention and therapy

et al. 2009

View full text Add to dashboard Cite

Phytochemical research has revealed that organic sulfur-containing compounds (OSCs) from Allium species exert biological effects, that might be beneficial in the treatment or prevention of a range of diseases, such as infections, cardiovascular and metabolic affections, cancers and related indispositions. Focusing physiological activities of these compounds in the context of cancer, it became clear from both epidemiological studies in men and experimental studies in diverse models, that the OSCs have a strong potential to prevent or to treat cancers even with selectivity against non-neoplastic cells. Though underlying mechanisms are not yet fully understood, several parts of their modes and mechanisms of action were elucidated: Pivotal molecular targets of as well chemoprevention as chemotherapy are metabolic, transporter or repair enzymes strongly affecting cell death, proliferation and formation of metastases. Accordingly effects are not restricted to the run of cell death programs, but they moreover comprise the strongly interdepending immune and inflammatory systems. Respectively, several hypotheses exist which are based on chemical properties of sulfur as the ''pharmacophor'' of the compounds appearing in up to ten different oxidation states (-2 to ?6). Hence compounds can undergo redox-reactions and electrostatic interactions, making reactive oxygen species (ROS) a key feature of their mechanisms of action.

show abstract

Cited by 135 publications

References 31 publications

Induction of apoptosis with diallyl disulfide in AGS gastric cancer cell line

Induction of apoptosis with diallyl disulfide in AGS gastric cancer cell line

Cell cycle arrest in early mitosis and induction of caspase-dependent apoptosis in U937 cells by diallyltetrasulfide (Al2S4)

Potential role of organic sulfur compounds from Allium species in cancer prevention and therapy

Contact Info

Product

Resources

About