Induction of apoptosis and G₂/M cell cycle arrest by Scrophularia striata in a human leukaemia cell line

Abstract

Results of the study indicated that S. striata extract could inhibit leukaemia cell proliferation by inducing G2 /M phase arrest and apoptosis.

Apoptosis Cell Death Effect of Scrophularia Variegata on Breast Cancer Cells via Mitochondrial Intrinsic Pathway

“…Moreover, some studies showed that other genus of scrophularia such as S. floribunda and S. striata extract induced apoptosis in tumor cells through induction of cell cycle arrest. 3,13 Our findings demonstrated that S. variagata extract can induce a G2/M phase cell cycle arrest in MCF-7 cells in a dose-dependent manner.…”

“…To confirm breast cancer apoptosis, we evaluated the fragmented DNA from MCF-7 cells by gel electrophoresis as previously described. 3 In addition, caspase-3 and caspase-9 activity were assessed according to the manufacturer's instruction of the caspase colorimetric assay kit (R&D systems).…”

“…Our previous findings demonstrated that Scrophularia species had the anti-cancer activity by induction of apoptosis and inhibition of matrix metalloproteinases, anti-asthmatic, neuroprotective, inhibitory effect on the nitric oxide and pro-inflammatory cytokines production. [3][4][5][6][7][8][9][10] In the present study we investigated the cytotoxic effect and induction of apoptosis of S. variegata in the MCF-7 human breast cancer cell line.…”

The Effects of Scrophularia striata Extract on HEK Cell Line in Cell Culture

“…It has also been shown that S. striata extract could inhibit leukaemia cell proliferation by inducing apoptosis [15] . However, in contrast to this finding there are studies showing that Scrophularia striata extract had no effect on cell viability [17].…”

“…Also the studies indicated that S. striata extract could inhibit leukaemia cell proliferation by inducing G2 /M phase arrest and apoptosis . [15] Other findings indicated that both leaves and seeds of S.Striata contain both anti cancer and cell growth enhancing agents [16]. But in other studies showed that s.striata in spite of using in trating inflammatory diseases but Scrophularia striata extract had no effect on cell viability [17].…”