Induction of Apoptosis and Cell Cycle Blockade by Helichrysetin in A549 Human Lung Adenocarcinoma Cells

Ho, Yen Fong; Karsani, Saiful Anuar; Yong, Wai Kuan; Malek, Sri Nurestri Abd

doi:10.1155/2013/857257

Cited by 18 publications

(10 citation statements)

References 35 publications

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“…The characteristic morphological changes of the three cancer cells treated with AgNP and AuNP were spherical cells, cells detached from the culture or well plate, cell shrinkage, membrane blebbing, cytoplasmic condensation, bubbling and production of numerous cell surface protuberances at the plasma membrane. Similar changes in morphology of A-549, HEp-2 and MCF-7 cells have been reported by [46,47] and [48], respectively.…”

Section: Electrochemical Analysissupporting

confidence: 87%

Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy

2017

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Nanotechnology is an emerging field in science and technology, which can be applied to synthesize new materials at the nanoscale level. The present investigation aimed at comparing the synthesis, characterization and in vitro anticancer efficacy of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn. Silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were synthesized using aqueous extract of leaves with solution of silver nitrate (AgNO 3 , 1 mM) and chloroauric acid (HAuCl 4 •3H 2 O, 1 mM), respectively. The synthesized nanoparticles were characterized using UV-visible spectrophotometry, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy-dispersive analysis of X-rays, X-ray diffraction, thermogravimetric analysis and cyclic voltammetry, which confirmed the reduction of Ag + ions to Ag 0 and Au 3+ ions to Au 0. The in vitro anticancer efficacy of AgNPs, AuNPs and aqueous extract of leaves confirmed by MTT assay exhibited IC 50 concentrations of 28.125, 46.875 and 50 μg ml −1 for lung A-549 cells, 103.125, 34.375 and 53.125 μg ml −1 for HEp-2 cells and 62.5, 23.4 and 13.26 μg ml −1 for MCF-7 cells, respectively. The concentrations indicate that both silver and gold nanoparticles as well as aqueous extract of leaves exhibited high anticancer efficacy.

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Section: Electrochemical Analysissupporting

confidence: 87%

Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy

2017

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“…The antiproliferative assay was performed using an MTT assay according to the method of [ 15 ] with some modifications. Cells were plated into sterile 96-well culture plates at a density of 3.0 × 10 4 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

Hong

Lee

Rahman

et al. 2014

The Scientific World Journal

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Curcuma purpurascens Bl., belonging to the Zingiberaceae family, is known as temu tis in Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil of temu tis showed turmerone as the major component, followed by germacrone, ar-turmerone, germacrene-B, and curlone. The rhizome oil of temu tis exhibited strong cytotoxicity against HT29 cells (IC50 value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50 values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50 value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome's oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death.

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“…It had been reported that alterations in activated proteins (cyclins and cyclin-dependent kinases, etc. ), which led to failure of cell cycle arrest, may thus serve as markers of a more malignant phenotype and cell cycle-related genes aided in discrimination of atypical adenomatous hyperplasia from early adenocarcinoma [ 35 ]. Chen et al demonstrated that cell cycle progression effects on NF-κB activity represented a molecular basis underlying the aggressive tumor behavior [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Temporal Identification of Dysregulated Genes and Pathways in Clear Cell Renal Cell Carcinoma Based on Systematic Tracking of Disrupted Modules

Wang

Sun

Liu

et al. 2015

Computational and Mathematical Methods in Medicine

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Objective. The objective of this work is to identify dysregulated genes and pathways of ccRCC temporally according to systematic tracking of the dysregulated modules of reweighted Protein-Protein Interaction (PPI) networks. Methods. Firstly, normal and ccRCC PPI network were inferred and reweighted based on Pearson correlation coefficient (PCC). Then, we identified altered modules using maximum weight bipartite matching and ranked them in nonincreasing order. Finally, gene compositions of altered modules were analyzed, and pathways enrichment analyses of genes in altered modules were carried out based on Expression Analysis Systematic Explored (EASE) test. Results. We obtained 136, 576, 693, and 531 disrupted modules of ccRCC stages I, II, III, and IV, respectively. Gene composition analyses of altered modules revealed that there were 56 common genes (such as MAPK1, CCNA2, and GSTM3) existing in the four stages. Besides pathway enrichment analysis identified 5 common pathways (glutathione metabolism, cell cycle, alanine, aspartate, and glutamate metabolism, arginine and proline metabolism, and metabolism of xenobiotics by cytochrome P450) across stages I, II, III, and IV. Conclusions. We successfully identified dysregulated genes and pathways of ccRCC in different stages, and these might be potential biological markers and processes for treatment and etiology mechanism in ccRCC.

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Induction of Apoptosis and Cell Cycle Blockade by Helichrysetin in A549 Human Lung Adenocarcinoma Cells

Cited by 18 publications

References 35 publications

Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy

Comparative study of synthesized silver and gold nanoparticles using leaves extract of Bauhinia tomentosa Linn and their anticancer efficacy

Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

Temporal Identification of Dysregulated Genes and Pathways in Clear Cell Renal Cell Carcinoma Based on Systematic Tracking of Disrupted Modules

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