Induction of Apoplastic Invertase of Chenopodium rubrum by D-Glucose and a Glucose Analog and Tissue-Specific Expression Suggest a Role in Sink-Source Regulation

Roitsch, Thomas; Bittner, Michaela; Godt, Dietmute E.

doi:10.1104/pp.108.1.285

Cited by 216 publications

(160 citation statements)

References 54 publications

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“…This further supports the highly restricted expression pattern of the Nin88 promoter during anther development because we could not observe any effect during plant growth and development, and even anther formation was phenotypically normal. The high level of tissue specificity of the Nin88 promoter is supported further by the finding that antisense constructs of the extracellular invertases Cin1 from Chenopodium rubrum (36) and Ntbfruct1 from N. tabacum (47) under control of the Nin88 promoter also affected only pollen development (M.G. and T.R., unpublished observations), ruling out an interference of the corresponding antisense mRNA with invertases in other tissues.…”

Section: Discussionmentioning

confidence: 81%

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Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Goetz

Godt

Guivarc’h

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

298

250

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Extracellular invertase mediates phloem unloading via an apoplastic pathway. The gene encoding isoenzyme Nin88 from tobacco was cloned and shown to be characterized by a specific spatial and temporal expression pattern. Tissue-specific antisense repression of Nin88 under control of the corresponding promoter in tobacco results in a block during early stages of pollen development, thus, causing male sterility. This result demonstrates a critical role of extracellular invertase in pollen development and strongly supports the essential function of extracellular sucrose cleavage for supplying carbohydrates to sink tissues via the apoplast. The specific interference with phloem unloading, the sugar status, and metabolic signaling during pollen formation will be a potentially valuable approach to induce male sterility in various crop species for hybrid seed production.

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Section: Discussionmentioning

confidence: 81%

“…A 750-bp cDNA fragment of an extracellular invertase, designated Nin88, was cloned by reverse transcription-PCR (RT-PCR) by using RNA from tobacco anthers and the degenerate primers OIN3 and OIN4 (36). The Nin88 gene was cloned by screening of a tobacco genomic library in lambda gt10 with the Nin88 cDNA.…”

Section: Methodsmentioning

confidence: 99%

Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Goetz

Godt

Guivarc’h

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

298

250

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“…In a photoautotrophic cell-suspension culture of C. rubrum, the addition of Glc or Suc induced the expression of genes encoding extracellular invertase and Suc synthase. This induction could be mimicked by 6-dGlc (Godt et al, 1995;Roitsch et al, 1995). The induction by 6-dGlc suggests that nonphosphorylated Glc is the signal for the sugar-induced expression of both genes.…”

Section: Sugar Transporters and Sugar Sensingmentioning

confidence: 89%

Sugar Sensing and Sugar-Mediated Signal Transduction in Plants

Smeekens

Rook²

1997

Plant Physiology

552

577

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Information concerning the sugar status of plant cells is of great importance during a11 stages of the plant life cycle. The availability of or lack of sugars triggers many metabolic and developmental responses, and it is not surprising, therefore, that sugars profoundly affect the expression of a large number of genes (for review, see Koch, 1996;Graham, 1996). Sugar sensing occurs at the level of individual cells and the responses of such cells must be integrated at the tissue, organ, and plant level. Therefore, sugar-induced signals will interact with other sensing and signaling pathways. The mechanisms used by plant cells to sense sugars and to process this information are essentially unknown, and only recently are these questions being addressed experimentally. This lack of knowledge contrasts with the situation in yeast and bacteria, in which the molecular and physiological analysis of mutants have yielded extensive information about sugar perception (Trumbly, 1992;Ronne, 1995;Saier et al., 1995). SUGAR SENSING IN YEAST A N D ANIMALSYeast (Sacckauomyces ceuevisiae) serves as a model for investigating many basic biological questions about eukaryotes and is also an important paradigm for sugar sensing in plants. In yeast the availability of the preferred sugar substrate Glc signals the Glc repression phenomenon (for review, see Trumbly, 1992;Ronne, 1995;Thevelein and Hohmann, 1995). Glc repression dramatically alters yeast intermediary carbohydrate metabolism such that only Glc is being used as a carbon source, despite the presence of other readily accessible carbon sources. Glc is converted into Glu-6-P by HXK and is further metabolized via glycolysis. Genes involved in the metabolism of other carbon substrates are switched off, as are genes encoding key steps in gluconeogenic metabolism. A number of yeast mutants that are impaired in aspects of the Glc repression phenomenon have been isolated and their analysis has provided insight into the complexity of sugar sensing and signaling pathways. From these studies it was concluded that the Glc-phosphorylating enzyme HXK2 is a major Glc sensor responsible for sus- tained Glc repression. HXK2 activity initiates a signal transduction pathway that involves a number of different gene products (Fig. 1) and results in the repression of a large set of genes. Thus, the entry of Glc into glycolytic metabolism as mediated by HXK2 is a key step in Glc sensing.In the repression pathway the function of two protein complexes has been elucidated. These are the GLC7 type 1 protein phosphatase complex (Tu and Carlson, 1995, and refs. therein) and the SSN6/TUP1 complex, which functions as a general repressor of transcription through modulation of chromatin structure. Binding of the SSN6/TUP1 complex to specific sites is directed by the DNA-binding protein MIG1, and in this way genes that contain MIG1-binding sites are repressed. Exactly how the HXK2, GLC7, and SSNG/TUPl /MIG1 complexes are connected is unknown. For example, in the repression pathway no substrates for the REG1 ...

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“…This induction by glucose could be mimicked by the nonmetabolizable glucose analogue 6-deoxyglucose. This glucose analogue is not a substrate for hexokinase, suggesting that the non-phosphorylated glucose is the signalling molecule for this induction Roitsch et al, 1995).…”

Section: Introductionmentioning

confidence: 94%

Sucrose‐specific signalling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene

Rook¹,

Gerrits²,

Kortstee³

et al. 1998

The Plant Journal

238

186

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SummaryThe Arabidopsis bZIP transcription factor gene ATB2 has been shown previously to be expressed in a light-regulated and tissue-specific way. Here we describe the precise localization of ATB2 expression, using transgenic lines containing an ATB2 promoter-GUS reporter gene construct. The observed expression pattern suggests a role for ATB2 in the control of processes associated with the transport or utilization of metabolites. Remarkably, expression of the ATB2-GUS reporter gene construct was specifically repressed by sucrose. Other sugars, such as glucose and fructose, alone or in combination, were ineffective. Repression was observed at external sucrose concentrations exceeding 25 mM. Transcript levels of both the endogenous ATB2 gene and the ATB2-GUS reporter gene were not repressed by sucrose, suggesting that sucrose affects mRNA translation. This translational regulation involves the ATB2 leader sequence because deletion of the leader resulted in loss of sucrose repression. Our results provide evidence for a sucrose-specific sugar sensing and signalling system in plants.

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Induction of Apoplastic Invertase of Chenopodium rubrum by D-Glucose and a Glucose Analog and Tissue-Specific Expression Suggest a Role in Sink-Source Regulation

Cited by 216 publications

References 54 publications

Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Induction of male sterility in plants by metabolic engineering of the carbohydrate supply

Sugar Sensing and Sugar-Mediated Signal Transduction in Plants

Sucrose‐specific signalling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene

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