Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) and modulation of caspase-3 activity in different human cancer cells

Corbière, Cécile; Liagre, Bertrand; Terro, Faraj; Beneytout, Jean-Louis

doi:10.1038/sj.cr.7290219

Cited by 155 publications

(93 citation statements)

References 41 publications

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“…Mitochondria play an important role in apoptotic pathways resulted from a variety of events including the release of caspase activators, changes of the MMP, ROS generation, and regulation of Bcl-2 family proteins (Corbiere et al, 2004;Ekert et al, 1999). According to our data, significant loss of MMP was observed in cells co-treated with Wit A and IR (Fig.…”

Section: Discussionsupporting

confidence: 50%

Combination of withaferin A and X-ray irradiation enhances apoptosis in U937 cells

Yang

Choi

Kim

et al. 2011

Toxicology in Vitro

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Section: Discussionsupporting

confidence: 50%

Combination of withaferin A and X-ray irradiation enhances apoptosis in U937 cells

Yang

Choi

Kim

et al. 2011

Toxicology in Vitro

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“…Finally downregulation of AIF expression by AIF-siRNA diminished A3D8-induced cell death suggesting that AIF is required for CD44-induced in HEL cells. Recent studies have suggested that AIF is a central mediator of relevant experimental models of cell death (Corbiere et al, 2004;Kang et al, 2004;Liu et al, 2004a;Cheung et al, 2005;Ishitsuka et al, 2005;Park et al, 2005). However, AIF is a janus protein with a vital role in cells via its redox activity, and a lethal role by participating to chromatinolysis and chromatin condensation once translocated to the nucleus (Lipton and Bossy-Wetzel, 2002;Vahsen et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

CD44 ligation induces caspase-independent cell death via a novel calpain/AIF pathway in human erythroleukemia cells

et al. 2006

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Ligation of the cell surface molecule CD44 by anti-CD44 monoclonal antibodies (mAbs) has been shown to induce cell differentiation, cell growth inhibition and in some cases, apoptosis in myeloid leukemic cells. We report, herein, that exposure of human erythroleukemic HEL cells to the anti-CD44 mAb A3D8 resulted in cell growth inhibition followed by caspase-independent apoptosis-like cell death. This process was associated with the disruption of mitochondrial membrane potential (DWm), the mitochondrial release of apoptosis-inducing factor (AIF), but not of cytochrome c, and the nuclear translocation of AIF. All these effects including cell death, loss of mitochondrial DWm and AIF release were blocked by pretreatment with the poly (ADP-ribose) polymerase inhibitor isoquinoline. A significant protection against cell death was also observed by using small interfering RNA for AIF. Moreover, we show that calpain protease was activated before the appearance of apoptosis, and that calpain inhibitors or transfection of calpain-siRNA decrease A3D8-induced cell death, and block AIF release. These data suggest that CD44 ligation triggers a novel caspaseindependent cell death pathway via calpain-dependent AIF release in erythroleukemic HEL cells.

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“…PARP-1 is a member of the PARP family, which initiates DNA damage repair via the modification of poly adenosine diphosphate glycosylation receptor protein. PARP-1 has been shown to trigger apoptosis signaling and activate caspase-3 to induce cell apoptosis and DNA damage (24,29). Thus, we hypothesize that treatment with xanthohumol promoted PARP protein expression in laryngeal squamous cell carcinoma SCC4 cells.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that caspases are activated during tumor cell apoptosis (23). A series of novel caspase-related apoptosis-inducing pathways have been identified that may be targeted to control cancer, which have practical significance for the majority of tumors and thus, may result in breakthroughs with regard to the treatment of malignant tumors (24). The activation of caspase-9 and caspase-3 may underlie the apoptosis of fibroblasts in keloids (25).…”

Section: Discussionmentioning

confidence: 99%

Xanthohumol inhibits proliferation of laryngeal squamous cell carcinoma

Wang

Yin

et al. 2016

Oncology Letters

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Abstract. Xanthohumol is a flavonoid compound that exhibits antioxidant and anticancer effects, and is used to treat atherosclerosis. The aim of the present study was to investigate the effect of xanthohumol on the cell proliferation of laryngeal squamous cell carcinoma and to understand the mechanism of its action. The effects of xanthohumol on the cell viability and apoptosis rate of laryngeal squamous cell carcinoma SCC4 cells were assessed by Annexin V-fluorescein isothiocyanate/propidium iodide staining. In addition, the expression levels of pro-apoptotic proteins, caspase-3, caspase-8, caspase-9, poly ADP ribose polymerase (PARP) p53 and apoptosis-inducing factor (AIF), as well as anti-apoptotic markers, B-cell lymphoma 2 (Bcl-2) and myeloid cell leukemia 1 (Mcl-1), were analyzed by western blotting. The results revealed that treatment with 40 µM xanthohumol significantly inhibited the proliferation of SCC4 cells. Furthermore, xanthohumol treatment (40 µM) induced SCC4 cell apoptosis, as indicated by the significant increase in activity and expression of caspase-3, caspase-8, caspase-9, PARP, p53 and AIF. By contrast, the protein expression of Bcl-2 and Mcl-1 was significantly decreased following treatment with 40 µM xanthohumol. Taken together, the results of the present study indicated that xanthohumol mediates growth suppression and apoptosis induction, which was mediated via the suppression of Bcl-2 and Mcl-1 and activation of PARP, p53 and AIF signaling pathways. Therefore, future studies that investigate xanthohumol as a potential therapeutic agent for laryngeal squamous cell carcinoma are required.

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Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) and modulation of caspase-3 activity in different human cancer cells

Cited by 155 publications

References 41 publications

Combination of withaferin A and X-ray irradiation enhances apoptosis in U937 cells

Combination of withaferin A and X-ray irradiation enhances apoptosis in U937 cells

CD44 ligation induces caspase-independent cell death via a novel calpain/AIF pathway in human erythroleukemia cells

Xanthohumol inhibits proliferation of laryngeal squamous cell carcinoma

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