Induction of Antibodies Specific for Gp41 of HIV-1 by Gene Gun DNA Vaccination

Behrendt, Rayk; Fiebig, Uwe

doi:10.4172/2157-7560.1000145

Cited by 3 publications

(2 citation statements)

References 46 publications

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“…The same was true when a DNA immunisation using a vector allowing expression of the TM protein on the cell surface to imitate the localization near a lipid membrane was performed. 32 This is in accordance with numerous other reports using gp41 or parts of it for immunisation. No or only a very weak neutralizing activity was found.…”

Section: Summary Of the Immunisation Experiments With The Tm Proteinssupporting

confidence: 80%

Immunising with the transmembrane envelope proteins of different retroviruses including HIV-1

Denner

2013

Human Vaccines & Immunotherapeutics

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Section: Summary Of the Immunisation Experiments With The Tm Proteinssupporting

confidence: 80%

Immunising with the transmembrane envelope proteins of different retroviruses including HIV-1

Denner

2013

Human Vaccines & Immunotherapeutics

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“…26,32 Briefly, 10 ll serum dilution was mixed with 90 ll virus (MOI = 1) and incubated for 30 min/37°C. C8166 cells or phytohemagglutinin (PHA) (5 lg/ml)-stimulated PBMCs (5 • 10 4 cells in 100 ll) were added and incubated for 65 h. Cells were pelleted and lysed as previously described 33 and provirus integration was assessed by duplex real-time PCR using an HIV-1 NL4-3 -specific probe and primers (Table 1).…”

Section: Virus Neutralization Assaysmentioning

confidence: 99%

Immunization with Hybrid Proteins Containing the Membrane Proximal External Region of HIV-1

Strasz

Morozov

Kreutzberger

et al. 2014

AIDS Research and Human Retroviruses

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The transmembrane envelope (TM) protein gp41 of HIV-1 is an attractive target when designing a vaccine to induce neutralizing antibodies. A few broadly neutralizing antibodies (2F5, 4E10, and 10E8) that target conserved epitopes in the membrane proximal external region (MPER) of gp41 have been isolated from infected individuals. However, attempts to induce such antibodies by immunizations with gp41 and Env derivatives containing the MPER were successful only to some extent. In contrast, immunizations with the ectodomain of the TM protein p15E of different gamma retroviruses resulted in the induction of neutralizing antibodies. These sera recognized epitopes located in the MPER and in the fusion peptide proximal region (FPPR) of p15E. Based on these results, both regions of p15E were substituted with the corresponding sequences derived from gp41 of HIV-1. Thus, four different hybrid antigens were produced. One of the inserted sequences contained the epitopes of 2F5 and 4E10 in the MPER; the other corresponded to the FPPR. Vaccination of rats, guinea pigs, and a goat induced binding antibodies directed against the FPPR of gp41 and the 2F5 epitope (ELDKWA) located in the MPER. Despite the exact recognition of the 2F5 epitope, no or very weak neutralization of HIV-1NL4-3 by the immune sera was demonstrated. Nonetheless, using the strategy of hybrid proteins, antibodies targeting the desired epitope were successfully induced.

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Vaccination studies with the mper of HIV-1 gp41 grafted into transmembrane protein of a gammaretrovirus

Strasz¹

2013

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Induction of Antibodies Specific for Gp41 of HIV-1 by Gene Gun DNA Vaccination

Cited by 3 publications

References 46 publications

Immunising with the transmembrane envelope proteins of different retroviruses including HIV-1

Immunising with the transmembrane envelope proteins of different retroviruses including HIV-1

Immunization with Hybrid Proteins Containing the Membrane Proximal External Region of HIV-1

Vaccination studies with the mper of HIV-1 gp41 grafted into transmembrane protein of a gammaretrovirus

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