Induction of Anti-Forssman Antibodies in the Hamster-to-Rat Xenotransplantation Model1

Brouard, Sophie; Bouhours, Danièle; Sébille, Fabien; Ménoret, Séverine; Jp, Soulillou; Vanhove, Bernard

doi:10.1097/00007890-200003270-00026

Cited by 12 publications

(12 citation statements)

References 18 publications

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“…Circulating anti-hamster antibodies in the recipient's blood were measured by a cell-based indirect enzyme-linked immunosorbent assay (CELISA), as described earlier (18). The Syrian hamster kidney cell line HAK (CCL-15, American Type Culture Collection, Manassas, VA, USA) was used as the antigenic target.…”

Section: Cellular Elisamentioning

confidence: 99%

Endothelial Cell Protection by Dextran Sulfate: A Novel Strategy to Prevent Acute Vascular Rejection in Xenotransplantation

Laumonier¹,

Mohaçsi²,

Matozan³

et al. 2004

American Journal of Transplantation

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We showed recently that low molecular weight dextran sulfate (DXS) acts as an endothelial cell (EC) protectant and prevents human complement-and NK cellmediated cytotoxicity towards porcine cells in vitro. We therefore hypothesized that DXS, combined with cyclosporine A (CyA), could prevent acute vascular rejection (AVR) in the hamster-to-rat cardiac xenotransplantation model. Untreated, CyA-only, and DXS-only treated rats rejected their grafts within 4-5 days. Of the hearts grafted into rats receiving DXS in combination with CyA, 28% survived more than 30 days. Deposition of anti-hamster antibodies and complement was detected in long-term surviving grafts. Combined with the expression of hemoxygenase 1 (HO-1) on graft EC, these results indicate that accommodation had occurred. Complement activity was normal in rat sera after DXS injection, and while systemic inhibition of the coagulation cascade was observed 1 h after DXS injection, it was absent after 24 h. Moreover, using a fluorescein-labeled DXS (DXS-Fluo) injected 1 day after surgery, we observed a specific binding of DXS-Fluo to the xenograft endothelium. In conclusion, we show here that DXS + + CyA induces long-term xenograft survival and we provide evidence that DXS might act as a local EC protectant also in vivo.

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Section: Cellular Elisamentioning

confidence: 99%

Endothelial Cell Protection by Dextran Sulfate: A Novel Strategy to Prevent Acute Vascular Rejection in Xenotransplantation

Laumonier¹,

Mohaçsi²,

Matozan³

et al. 2004

American Journal of Transplantation

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“…17 For a 2 ⌸ molecule with large rotational constant such as OH (BϷ19 cm Ϫ1 ), 18 and for moderate to high values of the rotational angular momentum, the electronic wave function in the ⌸(AЈ) levels is symmetric with respect to reflection in the plane of rotation of the molecule, while, in the ⌸(AЉ) levels, antisymmetric. 21,22 Butler and Wiesenfeld proposed a simple physical explanation for the nonstatistical electronic propensity they observed. 21,22 Butler and Wiesenfeld proposed a simple physical explanation for the nonstatistical electronic propensity they observed.…”

Section: Introductionmentioning

confidence: 99%

“…The following section reviews the statistical CC and CS models as applied previously to a single-PES description of the O( 1 D) ϩH 2 reaction. 15,[20][21][22] and the final section suggests some possible directions for future research. III, we present the results of our calculation of the four relevant electronic potential energy surfaces in the product arrangement ( 1 AЈ, 1 AЉ, 3 AЈ, and 3 AЉ; see Fig.…”

Section: Introductionmentioning

confidence: 99%

Product multiplet branching in the O(1D)+H2→OH(2Π)+H reaction

Alexander

Rackham

Manolopoulos

2004

The Journal of Chemical Physics

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The statistical model of atom-diatom insertion reactions is combined with coupled-states capture theory and used to calculate product multiplet-resolved integral cross sections for the title reaction. This involves an ab initio determination of the four electronic potential energy surfaces that correlate with the products ((1,3)A(') and (1,3)A(")), and an accurate description of the electronic and spin-orbit couplings between them. The dependence of the resulting cross sections on the final-state rotational quantum number shows a statistical behavior similar to that observed in earlier studies of the reaction in which only the lowest ((1)A(')) potential was retained. In addition, however, the present calculations provide information on the branching between the OH((2)Pi) multiplet levels. Although the two spin-orbit manifolds are predicted to be equally populated, we find a strong propensity for the formation of the Pi(A(')) Lambda-doublet states. These two predictions confirm the experimental results of Butler, Wiesenfeld, Gericke, Brouard, and their co-workers. The nonstatistical population of the OH Lambda-doublet levels is a consequence of the bond breaking in the intermediate H(2)O complex and is preserved through the multiple curve crossings as the products separate. This exit-channel coupling is correctly described by the present theory.

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“…No carbohydrate tested inhibited the cross-reactivity of anti-hamster and anti-porcine xenoantibodies with cells from other species, confirming that the antigens involved in this reaction are distinct from those targeting E. faecalis. Identification of carbohydrate antigens expressed on cells and involved in the rejection of hamster xenografts in rats has remained elusive despite substantiating significant changes like with anti-Forssman antigen antibodies [24]. We cannot rule out that most of the cross-reactivity of xenoantibodies with cells from other species is due to a polyreactivity to the structural nature of the antigen-binding site rather than to antigenic similarity [25,26].…”

Section: Discussionmentioning

confidence: 99%

Boosted Rat Natural Xenoantibodies Cross-React with <b><i>Enterococcus faecalis</i></b> by Targeting Melibiose and <smlcap>L</smlcap>-Rhamnose

2013

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Natural antibodies include a subset described as xenoantibodies considered to be directed at microorganisms and also cross-react with antigens of unrelated species. In this study, we generated T-cell-independent (TI) and T-cell-dependent (TD) xenoantibodies in Lewis rats with hamster and pig blood injections. TI anti-hamster and anti-pig IgM and IgG xenoantibodies cross-reacted with Enterococcus faecalis but not with Escherichia coli isolated from the blood of Lewis rats after cecal ligation and puncture (CLP). TI anti-pig IgM xenoantibodies also showed some reactivity with two human blood isolates of E. faecalis. In contrast, TD xenoantibodies did not show any reactivity with rat or human bacteria. TI and TD anti-hamster and anti-pig IgM and IgG xenoantibodies showed cross-reactivity with lymphocytes and endothelial cells from species distinct to that used for immunization. Glycan array analysis and inhibition assays identified antibodies against melibiose and L-rhamnose as mediators of anti-hamster and anti-porcine xenoantibody cross-reactivity with E. faecalis. A rise in TI anti-hamster and anti-pig xenoantibodies was accompanied by decreased survival of Lewis rats in a low-severity sepsis model of CLP. Therefore, TI xenoantibodies in the rat include anti-carbohydrate antibodies reactive to bacteria of endogenous flora. Enhancement of these antibodies may result in more severe infectious diseases caused by these microorganisms.

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Induction of Anti-Forssman Antibodies in the Hamster-to-Rat Xenotransplantation Model1

Cited by 12 publications

References 18 publications

Endothelial Cell Protection by Dextran Sulfate: A Novel Strategy to Prevent Acute Vascular Rejection in Xenotransplantation

Endothelial Cell Protection by Dextran Sulfate: A Novel Strategy to Prevent Acute Vascular Rejection in Xenotransplantation

Product multiplet branching in the O(1D)+H2→OH(2Π)+H reaction

Boosted Rat Natural Xenoantibodies Cross-React with <b><i>Enterococcus faecalis</i></b> by Targeting Melibiose and <smlcap>L</smlcap>-Rhamnose

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