Induction of anamnestic T cell proliferation by antigen‐pulsed, bone marrow‐derived macrophages

Abstract

Bone marrow-derived macrophages (BMM phi) were grown in a liquid culture system in the presence of L cell-conditioned medium as a source of colony-stimulating factor. After a 4-h pulse with antigen, cultured irradiated BMM phi were capable of presenting the antigen to primed T cells as assessed in a T cell proliferation assay. Proliferation was optimal when BMM phi were used between days 5 and 8 of bone marrow cell culture. T cells of Lyt1 and Lyt123 phenotype had to be present at the start of the culture period to yield an optimal response. Conventional antisera and monoclonal antibodies directed against the H-2 I region and the I-A subregion, respectively, proved inhibitory in this system. Cultured BMM phi from low-responder strains failed to present antigens under immune response gene control in a form that was immunogenic to T lymphocytes. Cultured BMM phi might thus serve as a source of antigen-presenting cells in the study of cell-cell interaction and immune response gene regulatory mechanisms.

Multiple mechanisms allowMycobacterium tuberculosisto continuously inhibit MHC class II-mediated antigen presentation by macrophages

“…These macrophages elicit maximal responses after 1-4 h and remain capable of eliciting responses at the same or slightly decreased levels for several more hours (22,23). Another feature of our model is dose dependence between exogenous antigen concentration and maximum number of resultant surface pMHC complexes (data not shown), which has also been observed experimentally with T cell responses (8,24).…”

Epitope specificity and Ia restriction of T cell responses to insulin in a system of complementing Ir genes: analysis with primed lymph node T cells and a long‐term cultured T cell line

“…The involvement of @-Ab X I-Ak)FI hybrid Ia complexes as restriction elements has recently been reported for several antigens including keyhole limpet hemocyanin [16], ovalbumin [17], the polypeptides poly-L-Glu-poly-D,L-Ala--poly-L-Lys (G-A-L) [18] and poly-L(Tyr,Glu)-poly-D,L-Ala-poly-L-Lys [(T,G)-A--L] [19], and BI [15]. However, recognition of antigen exclusively in the context of (I-Ab X I-Ak)F1-unique restriction elements was only observed with G-A--L since, analogous to the situation with PI, both the H-2' and H-2k parental strains are low responders to this antigen [18].…”

Granulocyte‐macrophage colony‐stimulating factor‐cultured bone marrow‐derived macrophages reveal accessory cell function and synthesis of MHC class II determinants in the absence of external stimuli

“…, Marburg, FRG). L929-Sup1 containing M-CSF activity, was prepared as described [4]. Rat recombinant I F N y [13] was kindly donated to us by Dr. H. Kirchner (DKFZ, Heidelberg, FRG) and was used for pulse treatment of BMMQ at a final concentration of 10 IUlml.…”

“…Clone 10-2. 16 [ll], producing monoclonal antibody (mAb) specific for the I-Ak f3 chain [12], was obtained from the American Type Culture Collection (Rockville, MD). This mAb was used in ascites form.…”

“…The capacity to serve as APC could be ascribed to bone marrow macrophages (BMMQ,), derived from in vifro cultures of BM cells in the presence of L929 fibroblast-conditioned medium (L929-Sup) as a source of macrophage-colony-stimulating factor (M-CSF) [2-71. Antigen-presenting accessory function is a transient property of cultured BMMQ,, increasing up to about 7 days of in vitro culture and vanishing at later culture periods [4]. However, BMMQ, grown for prolonged periods of time in virro can be induced to present antigen by treatment with interferon-y (IFN-y) [ Tntiated thymidine with peritoneal exudate cells, stimulated in vivo by i.p.…”