Induction of An Oral Sensitization Model in Rats

Camps-Bossacoma, Mariona; Abril-Gil, Mar; Franch, Àngels; Pérez-Cano, Francisco J.; Castell, Margarida

doi:10.2174/2212707002666150402225609

Cited by 12 publications

(27 citation statements)

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“…In order to find out what happened in Th1/Th2-associated antibody isotypes, the concentration of specific IgG1, IgG2a, IgG2b, IgG2c, and IgE antibodies was determined. The oral sensitization caused the synthesis of antibodies belonging to IgG1, IgG2a, and IgG2b isotypes, as previously reported [20,22]. The 0.5% hesperidin-enriched diet did not significantly modify the levels of these anti-OVA antibodies (Table 4).…”

Section: Resultssupporting

confidence: 83%

Influence of Hesperidin on the Systemic and Intestinal Rat Immune Response

et al. 2017

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Polyphenols, widely found in edible plants, influence the immune system. Nevertheless, the immunomodulatory properties of hesperidin, the predominant flavanone in oranges, have not been deeply studied. To establish the effect of hesperidin on in vivo immune response, two different conditions of immune system stimulations in Lewis rats were applied. In the first experimental design, rats were intraperitoneally immunized with ovalbumin (OVA) plus Bordetella pertussis toxin and alum as the adjuvants, and orally given 100 or 200 mg/kg hesperidin. In the second experimental design, rats were orally sensitized with OVA together with cholera toxin and fed a diet containing 0.5% hesperidin. In the first approach, hesperidin administration changed mesenteric lymph node lymphocyte (MLNL) composition, increasing the TCRαβ+ cell percentage and decreasing that of B lymphocytes. Furthermore, hesperidin enhanced the interferon (IFN)-γ production in stimulated MLNL. In the second approach, hesperidin intake modified the lymphocyte composition in the intestinal epithelium (TCRγδ+ cells) and the lamina propria (TCRγδ+, CD45RA+, natural killer, natural killer T, TCRαβ+CD4+, and TCRαβ+CD8+ cells). Nevertheless, hesperidin did not modify the level of serum anti-OVA antibodies in either study. In conclusion, hesperidin does possess immunoregulatory properties in the intestinal immune response, but this effect is not able to influence the synthesis of specific antibodies.

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Section: Resultssupporting

confidence: 83%

Influence of Hesperidin on the Systemic and Intestinal Rat Immune Response

et al. 2017

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“…and oral administration [ 42 ]. Although BN rats are high IgE responders, similar to atopic humans, experimental procedures in this rat strain to induce oral sensitization without adjuvant are time-consuming and are not always able to generate a reproducible and effective FA model [ 20 , 30 , 37 – 39 ]. In fact, we previously tested a model in BN rats administered only by oral route and the result was that a few animals were sensitized and none produced specific IgE [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…In this latter condition, Knippels et al have demonstrated oral sensitization and have evaluated the influence of rat strain [ 30 ] and dosage [ 31 , 32 ]. However, the model of oral sensitization without an adjuvant requires a long process of sensitization (six weeks) and, although it has been used in several studies [ 33 – 36 ], success after oral sensitization was not always achieved in a high percentage of rats [ 37 ] and/or the sensitization does not always induce the synthesis of IgE antibodies [ 20 , 30 , 38 , 39 ]]. This limitation makes it difficult to use this model for the screening of new therapies or allergenicity studies.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats

et al. 2015

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BackgroundFood allergy (FA) is an adverse health effect produced by the exposure to a given food. Currently, there is no optimal animal model of FA for the screening of immunotherapies or for testing the allergenicity of new foods.ObjectiveThe aim of the present study was to develop an effective and rapid model of FA in Brown Norway rats. In order to establish biomarkers of FA in rat, we compared the immune response and the anaphylactic shock obtained in this model with those achieved with only intraperitoneal immunization.MethodsRats received an intraperitoneal injection of ovalbumin (OVA) with alum and toxin from Bordetella pertussis, and 14 days later, OVA by oral route daily for three weeks (FA group). A group of rats receiving only the i.p. injection (IP group) were also tested. Serum anti-OVA IgE, IgG1, IgG2a, IgG2b and IgA antibodies were quantified throughout the study. After an oral challenge, body temperature, intestinal permeability, motor activity, and mast cell protease II (RMCP-II) levels were determined. At the end of the study, anti-OVA intestinal IgA, spleen cytokine production, lymphocyte composition of Peyer’s patches and mesenteric lymph nodes, and gene expression in the small intestine were quantified.ResultsSerum OVA-specific IgG1, IgG2a and IgG2b concentrations rose with the i.p. immunization but were highly augmented after the oral OVA administration. Anti-OVA IgE increased twofold during the first week of oral OVA gavage. The anaphylaxis in both IP and FA groups decreased body temperature and motor activity, whereas intestinal permeability increased. Interestingly, the FA group showed a much higher RMCP II serum protein and intestinal mRNA expression.ConclusionsThese results show both an effective and relatively rapid model of FA assessed by means of specific antibody titres and the high production of RMCP-II and its intestinal gene expression.

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“…The FA, regardless of the cocoa diets, did not modify the gene expression of either epithelial barrier proteins involved in luminal protection of the intestine. This fact must be attributed to the method of FA induction, which, unlike other models [38],…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Effect of a cocoa-enriched diet on immune response and anaphylaxis in a food allergy model in Brown Norway rats

Abril-Gil

Pérez-Cano

Franch

et al. 2016

The Journal of Nutritional Biochemistry

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Previous studies have demonstrated that cocoa intake decreased Th2 immune-related antibodies in rats. In consequence, we aimed to study in depth this cocoa action, particularly assessing its effect on a rat model of food allergy (FA) and also on an anaphylactic response. The involvement of the intestinal immune system was analyzed to allow the action mechanisms to be investigated. The role of cocoa flavonoids in the antiallergic properties of cocoa was also established. Brown Norway rats were fed either a reference diet or diets containing conventional cocoa (CC) or nonfermented cocoa (NFC). FA to ovalbumin (OVA) was induced and, later, an anaphylactic response was provoked. As expected, the synthesis of anti-OVA IgE and other Th2-related antibodies was inhibited by CC diet. In addition, the release of mast cell protease II after anaphylaxis was partially prevented by CC, although other variables were not modified. The CC diet also attenuated the increase of some Th2-related cytokines released from mesenteric lymph node and spleen cells, and modulated the intestinal gene expression of molecules involved in allergic response. These results demonstrated the local and systemic influence of CC diet. The effects of the NFC diet were weaker than those of CC, suggesting that cocoa components other than flavonoids play a role in cocoa's action. In conclusion, by acting on intestinal and systemic immune functions, a cocoa-enriched diet in rats exhibited a protective effect against FA and partially against anaphylaxis, making this a food of high interest to the fields of health and immunonutrition.

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Induction of An Oral Sensitization Model in Rats

Cited by 12 publications

References 0 publications

Influence of Hesperidin on the Systemic and Intestinal Rat Immune Response

Influence of Hesperidin on the Systemic and Intestinal Rat Immune Response

Development and Characterization of an Effective Food Allergy Model in Brown Norway Rats

Effect of a cocoa-enriched diet on immune response and anaphylaxis in a food allergy model in Brown Norway rats

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