Induction of alkaline phosphatase in Escherichia coli: effect of procaine hydrochloride

Tribhuwan, R C; Pradhan, D.S.

doi:10.1128/jb.131.2.431-437.1977

Cited by 29 publications

(8 citation statements)

References 17 publications

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“…Therefore, short-term treatment with procaine has no effect on the maturation of the majority of periplasmic components. In the case of alkaline phosphatase, procaine addition to phosphate-starved cells blocked the appearance of the enzyme activity (28). We have confirmed that observation in our strains (data not shown).…”

Section: Discussionsupporting

confidence: 91%

“…Procaine, a local anesthetic, is a membrane-active drug (18). It has been reported that 20 mM procaine does not inhibit either overall protein synthesis or induction of /3-galactosidase (28). Therefore, any effect on the growth rate of cells in the presence of 20 mM procaine and a particular carbon source might reflect action of the drug on the entry of carbohydrate into the cell.…”

Section: Resultsmentioning

confidence: 99%

“…Local anesthetics alter the function of membranes in both eucaryotic (19,22,23) and procaryotic cells (1,9,10,14,20,25,28), but their mechanism of action is unclear. Disruption of membrane function may be due to perturbation of the lipid bilayer structure or to direct interaction with target proteins (22).…”

mentioning

confidence: 99%

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Selective inhibition of carbohydrate transport by the local anesthetic procaine in Escherichia coli

Granett¹,

Villarejo²

1981

J Bacteriol

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Maltose and lactose transport systems have been used to investigate the action of procaine on insertion and activity of membrane proteins and translocation of exported proteins in Escherichia coli. Procaine mildly inhibited growth on lactose. The level of inhibition was consistent with the small reduction observed in active and facilitated transport functions of the lac permease. However, procaine caused a severe reduction of growth rate on maltose, as well as an inhibition of induction of maltose regulon activities. In both constitutive and inducible strains, the synthesis of both maltose transport activity (malB operon) and amylomaltase activity (malA operon) was inhibited. Coordinate inhibition of soluble and membrane products was not observed with the lac operon. 8B-Galactosidase synthesis proceeded normally during growth on procaine, whereas, the appearance of new transport activity was reduced. Regardless of carbon source, procaine specifically inhibited the appearance of ompF protein in the membrane fraction. 57.7 mCi/mmol were obtained from Amersham Corp. Acrylamide, methylenebisacrylamide, and sodium dodecyl sulfate (SDS) were purchased from Bio-Rad Laboratories, Richmond, Calif. Procaine was purchased from Chemical Dynamics Corp., South Plain-289 on July 31, 2020 by guest

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

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Selective inhibition of carbohydrate transport by the local anesthetic procaine in Escherichia coli

Granett¹,

Villarejo²

1981

J Bacteriol

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“…Treatment of wild-type strains with the local anesthetic procaine mimics envZ missense mutations. Procaine causes a PhoA-MalE-LamB-OmpFphenotype by blocking transcription of these genes (5,6,19,25). Missense mutants resistant to procaine action map in envZ (24).…”

mentioning

confidence: 99%

envZ mediates transcriptional control by local anesthetics but is not required for osmoregulation in Escherichia coli

Villarejo¹,

Case²

1984

J Bacteriol

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Expression of a particular set of exported protein genes (ompF, ompC, phoA, and malE) can be specifically altered in three ways: variation in the osmotic strength of the growth medium, mutations in the regulatory locus envZ, or treatment with sublethal concentrations of the local anesthetic procaine. To clarify relationships among these factors in the regulation of transcription, expression of the affected genes was compared in envZ+ and envZ22(Am) mutant strains grown in media of differing osmolarities with and without procaine. Loss of the envZ product resulted in complete resistance of gene expression to procaine, supporting the hypothesis that envZ mediates procaine inhibition. The specific activity of the phoA product, alkaline phosphatase, was elevated in envZ22 mutant strains, while the amounts of both the OmpC and OmpF porins were reduced. Osmotic control of phoA and ompC was retained in the absence of envZ function, but osmoregulation of ompF was lost. Therefore, the envZ product is somehow involved in the complex regulation of all four target genes, but is not solely responsible for osmoregulation.

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“…The administration of sub-lethal concentrations of procaine to E. coli causes pleiotropic alterations in the synthesis of a wide variety of outer membrane and periplasmic proteins [6][7][8][9][10]. We were interested in establishing whether sub-lethal concentrations of procaine would interfere with the stable maintenance of pSC101 by inhibiting the synthesis of a chromosomally encoded membrane protein required for par function.…”

Section: Introductionmentioning

confidence: 99%

The effect of procaine on the partitioning of plasmid pSC101

Lancaster

Prior

Mann

1986

FEMS Microbiology Letters

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The effect of procaine at a sub‐lethal concentration (20 mM) on the segregational stability of pSC101 and other plasmids in Escherichia coli was examined in phosphate‐limited chemostat culture. Procaine caused segregational instability of pSC101 and a derivative of pBR322 carrying the par function of pSC101. The segregational stability of 2 other unrelated plasmids, RP4 and pDS1109, was unaffected by procaine. It is concluded that the synthesis of a chromosomally encoded membrane protein, under the control of the envZ gene product, is required for pSC101 par function or that the functioning of this protein is impaired by the presence of procaine.

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Induction of alkaline phosphatase in Escherichia coli: effect of procaine hydrochloride

Cited by 29 publications

References 17 publications

Selective inhibition of carbohydrate transport by the local anesthetic procaine in Escherichia coli

Selective inhibition of carbohydrate transport by the local anesthetic procaine in Escherichia coli

envZ mediates transcriptional control by local anesthetics but is not required for osmoregulation in Escherichia coli

The effect of procaine on the partitioning of plasmid pSC101

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