Induction of a Stable Phagocytic Property by Cytosine Arabinoside in an SV40‐Transformed Macrophage Cell Line

Tanigawa, Tetsuya; Takayama, Hisao; Takagi, Atsushi

doi:10.1111/j.1348-0421.1980.tb02880.x

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…The reason for the difference in cellular behavior in these two conditions remains to be found. Phagocytic activity is fully expressed also in slowly growing cultures prepared by replating the SV40-transformed macrophages in a plastic petri dish for bacterial culture at high and lower densities where cell spreading is insufficient (Takayama et al, 1981b), and in the slowly growing variant cells derived from the SV404ransformed macrophages (Tanigawa et al, 1980). The rapidly growing cells at lower and at relatively high densities fail to retain their phagocytic activity (Takayama, 1980;Figs. 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

“…That is, in cultures at a moderate cell density, cells grow rapidly, show intense expression of SV40 T antigen in their nuclei by immunofluorescence, and lose their macrophage properties; whereas when cultures become confluent, the cells do not increase in number, express T antigen only weakly in their nuclei, and regain macrophage properties such as immune phagocytosis. Sublines isolated from the transformed macrophage lines having a much lower growth rate than that of the paren-tal line stably retain their macrophage properties throughout the period of cultivation (Tanigawa et al, 1980(Tanigawa et al, , 1982. These observations suggest that there may be a correlation among expression of the differentiated functions of macrophage, cell growth rate and, possibly, SV40 T antigen expression.…”

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confidence: 85%

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Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus40. I. Cellular response in proliferative and phagocytic activities to the shift of temperature differs depending on the culture state in mouse bone marrow cells transformed by the tsA640 mutant of simian virus 40

Tanigawa

Takayama

Takagi

et al. 1983

Journal Cellular Physiology

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It was shown previously that mouse bone marrow cells transformed by simian virus 40 (SV40) show a reversible cell density-dependent phenotypic transition between the nonmacrophage (rapidly growing) and the macrophage (stationary) states; cells in low-density cultures are in the growing phase, express SV40 T antigen strongly as revealed by immunofluorescence, and lose typical macrophage properties such as immune phagocytosis; whereas cells in high-density cultures are in the stationary (nongrowing) phase, express SV40 T antigen weakly, and recover their macrophage properties (Takayama, 1980). In the hope of clarifying the relationship between T antigen, cell growth, and macrophage-specific cellular function, we examined the behavior at 33 and 39 degrees C of mouse bone marrow cells transformed by an SV40 gene A mutant (tsA640) whose mutation renders the molecular weight of 90K (large) T antigen temperature sensitive. The results presented in this paper suggest that functional large T antigen is required for cells in the stationary phase to initiate multiplication when transferred at lower density and is not necessary for a majority of them to maintain the nongrowing state (viability) at both high and lower cell densities, whereas it is required for cells in the growing phase to keep multiplying without losing their viability. The results also suggest that the functional large T antigen does not play a direct role in maintaining the cells as either phagocytic or nonphagocytic. It is also suggested that the physiological or tsA mutation-mediated arrest of growth may or may not be accompanied by induction and/or maintenance of cellular phagocytic activity depending on the culture state.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 85%

Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus40. I. Cellular response in proliferative and phagocytic activities to the shift of temperature differs depending on the culture state in mouse bone marrow cells transformed by the tsA640 mutant of simian virus 40

Tanigawa

Takayama

Takagi

et al. 1983

Journal Cellular Physiology

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“…Change in Prostaglandin Levels in Culture Medium qf 28-12(Ara) Cells 28-12(Ara) cells, a subline isolated by cultivation with Ara-C, have been shown to have a reduced growth rate and a stably phagocytic nature (25). This line was cultivated without a change in the medium for 4 days.…”

Section: Change In Prostaglandin Levels In Culture Medium During a Phmentioning

confidence: 99%

“…That is, rapidly growing cells lose macrophage properties such as phagocytosis, but stationary cells gradually regain the properties. Furthermore, we reported that a subline which stably maintained its phagocytic nature could be isolated from the parental line 28-12 by culturing the cells in the presence of cytosine arabinoside (Ara-C) (25).…”

mentioning

confidence: 99%

Changes in Prostaglandin Levels in Cultures of SV40‐Transformed Macrophage Cell Lines in Relation to Their Phenotypic Expression

Tanigawa

Suzuki

Takayama

et al. 1982

Microbiology and Immunology

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A radioimmunoassay was performed to determine the total amounts of the A, B, and E series prostaglandins (prostaglandins) in culture fluids of simian virus 40 (SV40)-transformed mouse clonal macrophages, line 28-12, and its subline, 28-12(Ara). When the proportion of phagocytic cells in confluent 28-12 cell cultures increased, the prostaglandin levels in the culture fluids decreased. On the other hand, stably phagocytic 28-12(Ara) cells, which were derived from 28-12 cells and which had a reduced growth rate, did not release prostaglandins under the usual culture conditions; however, when they were treated with lipopolysaccharide or streptococcal preparation OK-432, large amounts of prostaglandins were released. In contrast, nonphagocytic cell populations in the cultures of 28-12 cells were not responsive to the drug treatment. These results suggest that there is a correlation between the phenotypic change from the nonphagocytic to the phagocytic state and a decrease in prostaglandin levels in culture fluids, and indicate that phagocytic cells are responsive to prostaglandin inducers, whereas nonphagocytic cells are not.

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Induction of endoreduplication in Chinese hamsters V79 cells by cytosine arabinoside

Ban

Iida

Shimba

et al. 1983

Biochemical and Biophysical Research Communications

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Induction of a Stable Phagocytic Property by Cytosine Arabinoside in an SV40‐Transformed Macrophage Cell Line

Cited by 4 publications

References 13 publications

Changes in Prostaglandin Levels in Cultures of SV40‐Transformed Macrophage Cell Lines in Relation to Their Phenotypic Expression

Induction of endoreduplication in Chinese hamsters V79 cells by cytosine arabinoside

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