Induction of a new enzyme activity to excise pyrimidine dimers in Escherichia coli infected with bacteriophage T4

Ohshima, Shoko; Sekiguchi, Mutsuo

doi:10.1016/0006-291x(72)90951-5

Cited by 24 publications

(2 citation statements)

References 10 publications

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“…1974), neither of which are associated with DNA polymerases, promote dimer excision from specifically preincised DNA in vitro. Enzyme activities comparable to some of these have been reported in extracts of M. luteus (Kaplan et al, 1969(Kaplan et al, , 1971Hamilton et al, 1973), and, in phage T4 infected E. coli, two phage-coded dimer excising 5' -* 3' exonucleases have been identified (Ohshima & Sekiguchi, 1972;Shimizu i;Sekiguchi, 1976).…”

supporting

confidence: 52%

Multiple thymine dimer excising nuclease activities in extracts of human KB cells

Cook¹,

Friedberg²

1978

Biochemistry

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Crude extracts of human KB cells grown in suspension culture contain enzyme activity that catalyzes the preferential excision of thymine-containing pyrimidine dimers from UV-irradiated E. coli DNA specifically incised adjacent to dimer sites. Fractionation of KB cell crude extracts reveals the presence of three such activities with distinct affinities for both DEAE-cellulose and phosphocellulose. One of the activities (activity B) is distinguished by its s 20,w (2.6) and isoelectric point (9.0) from the other two (activities A and C) which have similar s 20,w's (3.0-3.2) and isoelectric points (6.0). All three differ in their extent of stimulation by divalent cation and inhibition by NaCl or a sulfhydryl group inhibitor. These results indicate that multiple 5' leads to 3' dimer excision nuclease activities exist in human cells; however, there is as yet no direct evidence that these enzymes are functional in nucleotide excision repair in vivo.

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supporting

confidence: 52%

Multiple thymine dimer excising nuclease activities in extracts of human KB cells

Cook¹,

Friedberg²

1978

Biochemistry

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“…Other T4 phage-induced nucleases have been described. Endonuclease V (89,178,179) and exonuclease B (33,97) have a demonstrated role in repair of DNA which was damaged by ultraviolet light. Endonucleases I (3, designated endonuclease I later [172]), III (112), and VI (65) and the exonuclease activity associated with T4induced DNA polymerase (41,140) all attack both phage and bacterial DNA.…”

Section: Degradation Of Host Deoxyribonucleicmentioning

confidence: 99%

Shutoff of host macromolecular synthesis after T-even bacteriophage infection.

Koerner¹,

Snustad²

1979

Microbiological Reviews

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Despite all that is known about T4 infection, there is still no explanation why host macromolecular synthesis stops so promptly and completely. This remains one of the major unsolved questions of molecular biology. One suggestion is that an effect on the membrane by phage penetration secondarily influences the replication and transcription of the host chromosome tied to the membrane. Other evidence invokes a phage-induced protein whose effect is not defined. The attraction of this problem is not so much a compulsive need to complete the catalogue of events surrounding a phage infection, but more the promise of clarifying the spatial organization and control of the E. coli chromosome and with it another major insight into molecular biology."-A. Kornberg (72)

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Reproduction of Large Virulent Bacteriophages

Mathews¹

1977

Comprehensive Virology

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Induction of a new enzyme activity to excise pyrimidine dimers in Escherichia coli infected with bacteriophage T4

Cited by 24 publications

References 10 publications

Multiple thymine dimer excising nuclease activities in extracts of human KB cells

Multiple thymine dimer excising nuclease activities in extracts of human KB cells

Shutoff of host macromolecular synthesis after T-even bacteriophage infection.

Reproduction of Large Virulent Bacteriophages

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