Induction of a heparin-stimulated serine proteinase in sex accessory gland tumors of the Lobund-Wistar rat

Wilson, Michael J.; Lind, Jeremy; Sinha, Akhouri A.

doi:10.1016/j.yexmp.2015.04.008

Cited by 3 publications

(3 citation statements)

References 44 publications

(54 reference statements)

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“…Only few other groups have used the Lobund-Wistar model 37,51,52 and there are no reports on this model after 2015. 53…”

Section: Discussionmentioning

confidence: 99%

“…Only few other groups have used the Lobund-Wistar model 37,51,52 and there are no reports on this model after 2015. 53 Other similar models have been reported but have not been used by others than the authors of those reports, Pour and Stepan 54 and Pour et al 55 and Shirai and coworkers. [56][57][58] The carcinogen 3,2'-dimethyl-4-aminobiphenyl (DMAB) followed by testosterone propionate-filled Silastic implants induced adenocarcinomas in the dorsolateral prostate and seminal vesicle in Fischer F344 rats.…”

Section: Discussionmentioning

confidence: 99%

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The MNU Plus Testosterone Rat Model of Prostate Carcinogenesis

et al. 2022

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Animal models of prostate cancer are essential to identify chemopreventive treatments against this major male malignancy. The N-methyl- N-nitrosourea (MNU) plus testosterone rat model of prostate carcinogenesis is a reliable animal model that recapitulates human prostate cancer in many respects and has been used extensively in chemoprevention studies with good predictive value for the results of human clinical trials. The objective of this article is to describe the induction protocol of this model, demonstrate its robustness and reproducibility over time and across rat strains, provide diagnostic criteria for the identification of prostate lesions, and present the current tumor induction protocol so that others can use this model in a reliable manner. The majority of accessory sex gland tumors in this model are adenocarcinomas originating in the anterior and dorsolateral prostate that metastasize to lungs and abdominal structures. The rat strain used is of critical importance, with the commercially available Wistar WU and Fischer F344 strains yielding the highest tumor incidences. Low dose, long-term testosterone treatment is essential for a high tumor incidence, but in advanced stage, large adenocarcinomas do not appear to be androgen dependent. This rat model is a robust and reproducible prostate cancer animal model of human prostate cancer.

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“…Only few other groups have used the Lobund-Wistar model 37,51,52 and there are no reports on this model after 2015. 53…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The MNU Plus Testosterone Rat Model of Prostate Carcinogenesis

et al. 2022

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“…GO enrichment analysis showed that 22 upregulated mRNAs mainly regulated the activity of immune synapses, serine peptides, and chimeric antigen receptor T (CAR T) cells; rapidly recruited particles to immune synapses; separated from target cells more quickly; and drove cytotoxicity. Heparin-stimulated serine protease was induced in gonadal tumors of Lobund Wistar rats [ 61 ]. The analysis of the KEGG pathway showed that this pathway was enriched in IL12-mediated signal transduction, ZAP70 to immune synaptic enzyme, second messenger molecule production, and TCR signal transduction and was also involved in many cancers.…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Biomarkers of Platelet RNA in Glioblastoma by Bioinformatics Analysis

Chen

Lin

Jiang

et al. 2022

BioMed Research International

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Objective. Glioblastoma is one of the most common and fatal malignancies in adults. Current treatment is still not optimistic. Glioblastoma (GBM) transports RNA to platelets in the blood system via microvesicles, suggesting that platelet RNA can be a potential diagnostic and therapeutic target. The roles of specific platelet RNAs in treatment of GBM are not well understood. Methods. Platelet RNA profiling of 8 GBM and 12 normal samples were downloaded from the GEO database. Differentially expressed genes (DEGs) were identified between tumors and normal samples. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to elucidate the functions of up- and downregulated genes. miRNA was predicted by miRTarBase, TargetScan, and miRDB databases. circBase and circBank were used for circRNA prediction. ceRNA (circRNA-mRNA-miRNA) network was constructed to investigate the potential interactions. Results. 22 genes were upregulated and 9 genes were downregulated. There are only two genes (CCR7 and FAM102A) that connect to miRNAs (hsa-let-7a-5p, hsa-miR-1-3p). We assessed the overall survival rates by Kaplan-Meier plotter, and relative expression of GBM and subtypes for overlapped mRNA (CCR7 and FAM102A) were evaluated, and further, we obtained circRNAs (has-circ-0015164, hsa-circ-0003243) by circBank and circBase and bind sites through the CSCD database. Finally, a ceRNA network (circRNA-mRNA-miRNA) was constructed based on 2 miRNAs, 2 mRNAs, and 2 circRNAs by Cytoscape. This study focused on potential mRNA and ceRNA biomarkers to targeted treatment of GBM and provided ideas for clinical treatment through the combination of hematology and oncology. Conclusion. The findings of this study contribute to better understand the relationship between GBM and the blood system (platelets) and might lay a solid foundation for improving GBM molecule and gene diagnosis and prognosis.

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