Induction of a cDNA clone from rice encoding a class II small heat shock protein by heat stress, mechanical injury, and salicylic acid

Chang, Pi-Fang Linda; Jinn, Tsung-Luo; Huang, Wen-Kuan; Chen, Yuhsin; Chang, Hung-Chi; Wang, Chengwei

doi:10.1016/j.plantsci.2006.07.017

Cited by 39 publications

(27 citation statements)

References 43 publications

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“…A similar expression profile of all sHSP-CI genes at a lower level was suggested by ethanol, H 2 O 2 and CuCl 2 treatments (Guan et al, 2004 (Guan et al, 2003(Guan et al, , 2004. Chang et al (2007), for the first time, reported a fulllength cDNA clone for a class II sHSP, isolated from riceetiolated seedlings heat shocked at 41 o C for 2 h. The coding sequence consists of 501 bp, and the clone encodes a protein of 18.0 kDa. The obtained full-length cDNA clone, designated OsHSP18.0-CII, is almost identical to a putative class II sHSP gene located on rice chromosome 1 and another putative class II sHSP rice gene.…”

Section: Hsp60 Familysupporting

confidence: 58%

An overview on the small heat shock proteins

Mahmood¹,

Safdar²,

Abbasi³

et al. 2010

Afr. J. Biotechnol.

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In the last 25 years, a huge amount of literature has been accumulated describing the cell's response to different kinds of environmental stress conditions, such as high temperatures, altered pH, exposure of the cell to toxins, starvation, oxygen, and water deprivation, among others. Heat shock proteins (HSPs) are one of the main expressed products of the cell in response to stresses. HSPs can be classified into six structurally conserved classes according to their molecular weight namely, HSP100, HSP90, HSP70, HSP60, small heat shock proteins (sHSPs) and ubiquitin (8.5 kDa). In eukaryotes, different heat shock genes are expressed uncoordinatedly, whereas in prokaryote, heat shock genes form a regulon and appear simultaneously. sHSPs are associated with nuclei, cytoskeleton and membranes. They bind partially to denatured proteins, preventing irreversible protein aggregation during stress. In animals, only one sHSP gene has been located in yeast cells, ten in mammalian, two in birds and four genes have been found in Drosophila. However, in plants more than 20 sHSPs have been reported and they can be divided into 6 classes, of which, 3 classes (CI, CII and CIII) are in the cytosole or in the nucleus and the other three (CIV, CV and CVI) in the plastids, endoplasmic reticulum and mitochondria. Mitochondrial and chloroplast sHSPs protect electron transport chain. During development in animals, sHSP genes are normally regulated at late neurula and early tailbud stage and in plants during pollen development, seed maturation, seed imbibition and germination. Transcriptional regulation of sHSPs depends on particular activation of heat shock factors (HSF) which recognize the highly conserved heat-shock elements (HSEs). After the heat stress has been released, the sHSPs are quite stable, suggesting that sHSPs may be important for recovery as well.

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Section: Hsp60 Familysupporting

confidence: 58%

An overview on the small heat shock proteins

Mahmood¹,

Safdar²,

Abbasi³

et al. 2010

Afr. J. Biotechnol.

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“…In response to different environmental conditions like heat, cold, drought, anoxia and salt stress, rice plants express 23 sHSPs (Sarkar et al 2009). Of these, members of the CI subfamily, like Hsp16.9A (Chen et al 2014), Hsp18.0 (Guan et al 2004), Hsp18.0 of the CII subfamily (Chang et al 2007) and the chloroplast-localised Hsp26.7 (Lee et al 2000) have been investigated. Overexpression of rice Hsp16.9 (CI subfamily) in Escherichia coli conferred thermo-tolerance to the bacteria at temperatures that were otherwise considered lethal (Yeh et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Characterization of rice small heat shock proteins targeted to different cellular organelles

Mani

Ramakrishna

Suguna

2015

Cell Stress and Chaperones

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Small heat shock proteins (sHSPs) are a family of ATP-independent molecular chaperones which prevent cellular protein aggregation by binding to misfolded proteins. sHSPs form large oligomers that undergo drastic rearrangement/dissociation in order to execute their chaperone activity in protecting substrates from stress. Substrate-binding sites on sHSPs have been predominantly mapped on their intrinsically disordered N-terminal arms. This region is highly variable in sequence and length across species, and has been implicated in both oligomer formation and in mediating chaperone activity. Here, we present our results on the functional and structural characterization of five sHSPs in rice, each differing in their subcellular localisation, viz., cytoplasm, nucleus, chloroplast, mitochondria and peroxisome. We performed activity assays and dynamic light scattering studies to highlight differences in the chaperone activity and quaternary assembly of sHSPs targeted to various organelles. By cloning constructs that differ in the length and sequence of the tag in the N-terminal region, we have probed the sensitivity of sHSP oligomer assembly and chaperone activity to the length and amino acid composition of the N-terminus. In particular, we have shown that the incorporation of an N-terminal tag has significant consequences on sHSP quaternary structure.

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“…O marcador Q5VRY1 (LOC_Os01g08860) é associado a uma proteína de choque térmico, HSP18, (Chang et al, 2007). Essa classe de proteínas normalmente está envolvida na resposta a altas temperaturas e a outros estresses (Chandel et al, 2013).…”

Section: Resultsunclassified

Análise de associação quanto à produtividade e seus caracteres componentes em linhagens e cultivares de arroz de terras altas

Mendes

Borba

Bueno

et al. 2014

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi identificar, por meio da análise de mapeamento associativo, os marcadores moleculares relacionados à produtividade do arroz de terras altas e aos seus caracteres componentes. Foram usadas 113 linhagens e cultivares de arroz de terras altas, da Coleção Nuclear de Arroz da Embrapa, com reduzido vínculo genético entre si. Os seguintes caracteres componentes da produtividade foram avaliados: número de panículas por metro, número de grãos por panícula e peso de 100 grãos. Dos 115 marcadores utilizados, 25 (21,7%) associaram-se significativamente a um ou mais caracteres. Entre os 29 SSR ("simple sequence repeats") colocalizados em QTL ("quantitative trait loci") de produtividade de arroz, 12 foram associados aos caracteres avaliados e considerados como candidatos para uso na seleção assistida por marcadores. Os marcadores NP914540, Q6ZGD1 e Q69JE3, associados ao número de grãos por panícula, ainda não foram anotados no arroz e podem constituir o ponto de partida para estudos de genômica funcional. Entre os marcadores derivados de sequências transcritas, NP914526 e NP914533 destacam-se por pertencer a rotas metabólicas relacionadas ao aumento do potencial produtivo de arroz.Termos para indexação: Oryza sativa, coleção nuclear, desequilíbrio de ligação, genômica funcional, mapeamento associativo, seleção assistida por marcadores. Association analysis for yield and its component traits in lines and cultivars of upland riceAbstract -The objective of this work was to identify, through analysis of associative mapping, the molecular markers related to upland rice yield and its component traits. One hundred thirteen lines and cultivars of upland rice, with reduced admixture, from the Rice Core Collection of Embrapa, were used. The following yield component traits were evaluated: number of panicles per meter, number of grains per panicle, and weight of 100 grains. Out of the 115 used markers, 25 (21.7%) were significantly associated with one or more traits. Among the 29 SSR (simple sequence repeats) co-located in yield QTL (quantitative trait loci) in rice, 12 were associated with the evaluated traits and considered candidates for use in marker-assisted selection. The markers NP914540, Q6ZGD1, and Q69JE3, associated with the number of grains per panicle, are not yet annotated in rice and should constitute the starting point for functional genomics studies. Among the markers derived from transcribed sequences, NP914526 and NP914533 stand out for belonging to metabolic pathways related to increased yield potential of rice.

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Induction of a cDNA clone from rice encoding a class II small heat shock protein by heat stress, mechanical injury, and salicylic acid

Cited by 39 publications

References 43 publications

An overview on the small heat shock proteins

An overview on the small heat shock proteins

Characterization of rice small heat shock proteins targeted to different cellular organelles

Análise de associação quanto à produtividade e seus caracteres componentes em linhagens e cultivares de arroz de terras altas

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