Induction of 6-thioguanine-resistant mutants in human diploid fibroblats in vitro with ethylene oxide

Kolman, Ada; Bohušová, T.

doi:10.1016/0165-1161(92)91225-g

Cited by 2 publications

(3 citation statements)

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“…The genetic toxicity and animal carcinogenicity of EtO is well documented, and human occupational exposure to EtO has been associated with an increased risk of leukemia (36). The mutagenic effect of EtO in the HPRT gene in human diploid fibroblasts was demonstrated recently by Kolman et al (25). The mutant frequency was found to increase linearly within the dose range of 2.5 to 10 mM of EO, with a mutagenic potency of 9.8 x 10 6 mutants per mMh.…”

Section: Ethylene Oxide-induced Hprt Mutationmentioning

confidence: 92%

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Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.

Lambert

Andersson

Bastlová

et al. 1994

Environ Health Perspect

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Provisional mutational spectra at the hypoxanthine phosphoribosyl transferase (HPRT) locus in vitro have been worked out for acetaldehyde (AA) and benzo[alpyrene diolepoxide (BPDE) in human (T)-lymphocytes and for ethylene oxide (EtO) in human diploid fibroblasts using Southern blotting and polymerase chain reaction (PCR)-based DNA sequencing techniques. The results indicate that large genomic deletions are the predominating hprt mutations caused by AA and EO, whereas BPDE induces point mutations that are mainly GC>TA transversions. The mutational spectra induced by the three agents are clearly different from the background spectrum in human T-cells. Thus, the hprt locus is a useful target for the study of chemical-specific mutational events that may help identify causes of background mutation in human cells in vivo. -Environ Health Perspect 102(Suppl 4): 135-138 (1994).

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Section: Ethylene Oxide-induced Hprt Mutationmentioning

confidence: 92%

“…EtO-induced mutations were studied in human diploid VH-10 fibroblast cultures at early passages (25). Mutant dones were selected in mediums containing 6-thioguanine after the appropriate expression time of 8 to 10 days.…”

Section: Methodsmentioning

confidence: 99%

Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.

Lambert

Andersson

Bastlová

et al. 1994

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…Mutation induction of AA and BPDE was studied in freshly prepared human lymphocytes from male donors using the T-cell cloning method (22,23). EtO-induced mutations were studied in human diploid VH-10 fibroblast cultures at early passages (25). Mutant dones were selected in mediums containing 6-thioguanine after the appropriate expression time of 8 to 10 days.…”

Section: Methodsmentioning

confidence: 99%

Mutations Induced in the Hypoxanthine Phosphoribosyl Transferase Gene by Three Urban Air Pollutants: Acetaldehyde, Benzo[a]pyrene Diolepoxide, and Ethylene Oxide

Lambert¹,

Andersson²,

Bastlová³

et al. 1994

Environmental Health Perspectives

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Provisional mutational spectra at the hypoxanthine phosphoribosyl transferase (HPRT) locus in vitro have been worked out for acetaldehyde (AA) and benzo[a]pyrene diolepoxide (BPDE) in human (T)-lymphocytes and for ethylene oxide (EtO) in human diploid fibroblasts using Southern blotting and polymerase chain reaction (PCR)-based DNA sequencing techniques. The results indicate that large genomic deletions are the predominating hprt mutations caused by AA and EO, whereas BPDE induces point mutations that are mainly GC > TA transversions. The mutational spectra induced by the three agents are clearly different from the background spectrum in human T-cells. Thus, the hprt locus is a useful target for the study of chemical-specific mutational events that may help identify causes of background mutation in human cells in vivo.

show abstract

Induction of 6-thioguanine-resistant mutants in human diploid fibroblats in vitro with ethylene oxide

Cited by 2 publications

References 0 publications

Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.

Mutations induced in the hypoxanthine phosphoribosyl transferase gene by three urban air pollutants: acetaldehyde, benzo[a]pyrene diolepoxide, and ethylene oxide.

Mutations Induced in the Hypoxanthine Phosphoribosyl Transferase Gene by Three Urban Air Pollutants: Acetaldehyde, Benzo[a]pyrene Diolepoxide, and Ethylene Oxide

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