Induction, multiplication, and acclimatization of rodent tuber (Typhonium flagelliforme Lodd.) plant from Indonesia by in vitro organogenesis

Sianipar, Nesti Fronika; Laurent, Danny; Chelen,; Rosaria,; Tanty, Heruna

doi:10.1109/time-e.2015.7389748

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…There is an interaction between exogenous growth regulators supplied to the medium and endogenous growth regulators produced by plant tissues during the formation of organs like shoots or roots [29]. In a different experiment, rodent tuber grown in MS liquid medium with 3 mgL -1 BAP and 0.5 mgL -1 IBA produced the best number of shoots (4.4 shoots in 6 weeks) [30], and the same ratio between cytokinin and auxin was achieved with the maximum number of shoots obtained at the initiation stage of rodent tuber performed on MS medium with 0.5 mgL -1 BAP and 0.5 mgL -1 NAA (14 shoots) [31].…”

Section: The Effect Of Pgr Bap and Naa Combination On Rodent Tuber Sh...mentioning

confidence: 86%

“…By promoting cell elongation and division in the cambium tissue, auxin is often used to stimulate the production of callus, suspension cultures, and roots [32]. The best root production of the rodent tuber was induced by MS0 medium containing 1.5 mgL -1 NAA, which resulted in 43.20 roots [33].…”

Section: The Interaction Between Explant Type and Pgr Bap And Naa Com...mentioning

confidence: 99%

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Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Hardiany,

Fauzan,

Pramono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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A medicinal plant called rodent tuber has the potential to both prevent and treat various diseases. There is no Standard Operating Procedure (SOP) governing the growth method of rodent tuber because it still grows wild and is rarely cultivated. This research aimed to study the optimal growth of rodent tuber for mass seed propagation based on the type of explants and the concentration of auxin and cytokinin combinations for inducing shoots. While generative parameters (harvesting of tubers/shoots) were observed up to 13 WAP (Week After Planting), vegetative parameters were observed up to 10 WAP. The results showed that the best type of explants for shoot induction and growth of rodent tuber were whole plants with a survival rate of 100%; the number of shoots was 6.65; the plant height was 21.27 cm with 6.34 leaves; 26.56 roots with a length of 16.05 cm; and 40.80% flowering plants. PGR (Plant Growth Regulator) combination of 50 ppm BAP (Benzyl Amino Purine) and 100 ppm NAA (Naphthalene Acetic Acid) became the best explant soaking solution by forming eight shoots and the height of plants was 24.13 cm with seven leaves. The explant produced the highest root length in a combination of 100 ppm BAP and 50 ppm NAA, which was 17.70 cm. The interaction between whole plant explants immersed in 0 ppm BAP and 100 ppm NAA solution resulted in the most significant number of roots (41). Those goals can support the SOP of propagated methods and ensure the consistency, quality, and availability of raw materials for traditional medicine.

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Section: The Effect Of Pgr Bap and Naa Combination On Rodent Tuber Sh...mentioning

confidence: 86%

Section: The Interaction Between Explant Type and Pgr Bap And Naa Com...mentioning

confidence: 99%

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Hardiany,

Fauzan,

Pramono

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme

Rezali

Sidik

Saleh

et al. 2017

Asian Pacific Journal of Tropical Biomedicine

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The Effects of Subculture on The Mutant Plant Regeneration of Rodent Tuber (Typhonium flagelliforme) In Vitro Mutagenesis Using Gamma-Ray Irradiation

Sianipar

Assidqi

Abbas

2020

IOP Conf. Ser.: Earth Environ. Sci.

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The combination of gamma-ray irradiation treatment and somaclonal variation may cause morphological changes in vitro culture of rodent tuber. In vitro shoots were irradiated with several gamma-ray doses. Rodent tuber plantlets were irradiated with the doses of 20 Gy. The plantlets were subcultured up to 5 times then produced MV1 to MV5. Plantlets MV4 and MV5 were acclimatized and transplanted at the greenhouse. The purpose of this study was to analyze the morphological characteristics in the fourth mutant vegetative propagation (MV4) and the fifth mutant vegetative propagation (MV5) of rodent tuber from the combination of gamma-ray irradiation and somaclonal variation. Subcultures were performed to regenerate from MV1 to MV5 with optimal MS (Murashige & Skoog) medium. Subcultures were done at eight weeks. The morphological measurement in MV4 with the average number of shoots, number of leaves and plant height, i.e. 5.21, 14.41 and 5.52 cm, respectively. The highest average number of shoots, number of leaves and plant height was generated in MV5, i.e. 5.23, 16.54 and 5.18 cm, respectively. Pre-acclimatization were successfully produced in the greenhouse of about 30% (MV4) and 39.5% (MV5). Post acclimatization was obtained 100% in control, MV4, and MV5 at the greenhouse. MV5 had better morphological characteristics. The morphological changes were occurring in MV4 and MV5 compared with controls due to the combination of gamma-ray irradiation and somaclonal variation.

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Induction, multiplication, and acclimatization of rodent tuber (Typhonium flagelliforme Lodd.) plant from Indonesia by in vitro organogenesis

Cited by 3 publications

References 29 publications

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

Ex vitro shoot induction of rodent tuber (Typhonium flagelliforme) with benzyl amino purine and naphthalene acetic acid

The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme

The Effects of Subculture on The Mutant Plant Regeneration of Rodent Tuber (Typhonium flagelliforme) In Vitro Mutagenesis Using Gamma-Ray Irradiation

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