Induction mechanism of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality

Abstract

An efficient protocol for adventitious root induction from leaf explants of Morinda citrifolia treated with different concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) was established in relation to physiological process changes during adventitious root induction under different light sources (fluorescent, red, blue, red + blue, and far-red). Among the different concentrations of IBA and NAA, 1.0 mg l −1 IBA was proven as the best auxin source for adventitious root induction under fluorescent light. Higher concentrations of IBA and NAA trigger callus formation in both light and dark conditions. Maximum numbers of adventitious roots were induced under red light (26) followed by blue light (22) and the lowest under far-red light (6). In contrast, numerous callus formations were induced by red + blue followed by red and blue, while the highest root length (1.66 cm) with negligible callusing was observed under fluorescent light. Catalase and guaicacol peroxidase activities were highest under red light followed by fluorescent light and the lowest under red + blue light, but superoxide dismutase activity was not significantly influenced by different light sources. Ascorbate peroxidase played an important role in detoxification of the harmful effects of hydrogen peroxide (H 2 O 2 ). Under fluorescent light, significantly lower accumulation of H 2 O 2 was observed. Accumulation of H 2 O 2 in the induced root under different light showed a positive correlation with peroxidation of lipids and was observed higher under far-red followed by red + blue and blue light.

Sucrose regulated enhanced induction of anthraquinone, phenolics, flavonoids biosynthesis and activities of antioxidant enzymes in adventitious root suspension cultures of Morinda citrifolia (L.)

“…Plant material, induction and proliferation of adventitious root Adventitious roots were induced from leaf explants of in vitro grown plantlets of M. citrifolia (Baque et al 2010a). The plantlets were grown from mature seeds after sterilized with a 4% sodium hypochlorite solution for 20 min followed by soaking in a 2% sodium hypochlorite for 10 min in a laminar hood.…”

Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens

“…In another study, Praveen et al (2009) also found that NAA is more potent in triggering induction of adventitious roots from leaf explants of Andrographis paniculata compared to IBA and IAA. In contrast, IBA was found effective than NAA to induce adventitious roots from leaf segments of Morinda citrifolia (Baque et al 2010). This suggests that the response of root organogenesis to exogenous PGRs varies among plant species.…”

“…Regarding the plant organ cultures, root culture is more favorable than the shoot culture because of rapid root multiplication and the ability of cultivation in large-scale bioreactors. The adventitious root cultures induced by plant growth regulators (PGRs) have been studied in various medicinal plants species such as Valeriana amurensis (Cui et al 2011), Stevia rebaudiana (Reis et al 2011), and Morinda citrifolia (Baque et al 2010). In addition, adventitious root cultures of some medicinal plant species, e.g.…”

COMBINATION OF NAA AND TDZ FOR In Vitro MULTIPLICATION OF Eugenia involucrata DC

“…The supplementation of the nutritive medium with these compounds often results in callus induction in E. involucrata (Golle, 2010) and other species, such as Morinda citrifolia (Baque et al, 2010), Hyptis leucocephala (Pereira et al, 2012), Litchi chinensis (Ma et al, 2009), and Passiûora cincinnata (Silva et al, 2009;Pinto et al, 2010).…”