Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques

Abstract

Flow cytometry and stomata characteristics were used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata breeding clone SH-3362 . Culturing shoot tips in liquid medium supplemented both with 5 .0 mM colchicine for 48 hours or 30 pM oryzalin (3,5-dinitro-N4,N-dipropylsulphate) for seven days, both in combination with 2% (v/v) DMSO, resulted in a high (23 .1% and 29 .1%) frequency of non-chimeric tetraploids in the fourth vegetative generation . Although mixoploidy persisted in subsequent cycles of vegetative propagation, tetraploids as identified by flow cytometry remained solid non-chimeric during two more cycles . These autotetraploids were propagated for field testing. A rough pre-selection of regenerated V4 plants based on their stomata characteristics resulted in a population in which only 56.2% of the plants were solid tetraploids . The somatic polyploidization system reported here can be utilised for banana breeding programmes .

In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono- and interspecific diploid banana clones

“…Chimerical plants have often been obtained by various authors with banana [8,[12][13][14]. The production of "mixoploid" plants clearly supports the hypothesis of a pluri-cellular origin of the in vitro induction of shoot meristems in banana.…”

“…Stable autotetraploids have been established in the field, but reversions, formation of chimeras and aneuploids were common. Later, Van Duren et al [13] compared, in similar in vitro conditions, the efficiency of colchicine and oryzalin to induce polyploidisation on the same SH-3362 clone. Most effective in producing non-chimerical tetraploids was 30 µM oryzalin for 7 days or 5 mM colchicine for 48 h. More recently, Ganga et al [14] also reported the efficiency of colchicine in tetraploid induction of four mono-(AA) and interspecific (AB) Indian diploid banana cultivars.…”

Production of Colchicine Induced Tetraploids in Rye (Secale cereale L.)

“…최근 감자 (Ono & Hosaka, 2010), 수박 (Jaskani et al, 2005), 호밀 (Kubalakoba et al, 2003) (Jaskani et al, 2005), 헤어리베치 (Tulay & Unal, 2010), 바나나 (Duren et al, 1996) 등에서도 배수체가 얻어졌다는 보고 등으로 볼 때 본 시험에서 나온 결과는 향 후 호밀에서 배수성 품종을 육성하는데 도움이 될 수 있는 긍정적인 결과로 판단되었다. 또 배수체 작성 효율에서 4배 Mixoploids : Plants with diploid and tetraploid tillers in the same plant.…”

“…1990년 후반 부터 최근까지는 집단선발방법에 의해 올호밀 (Heo et al, 1998), 곡우호밀, 이그린, 올그린( (Heo et al, 2004(Heo et al, , 2009a(Heo et al, , 2009b (Geiger & Miedaner, 2009 ;Falke et al, 2008) 등 여러 가지 방법을 채택할 필요가 있다. 식물에서 배수성 육종은 염색체의 집합인 게놈의 양적인 배가를 통해 얻어지는 배수체를 소재로 하여 새로운 유용형 질의 출현이나 수량을 급진적으로 증대시키고자 하는 방법 으로써, 레드클로버(X=7), 알사이크 클로버(X=8), 턴닙(X=10), 시금치(X=6), 상추(X=9), 호밀 (X=7) 등에서 배수성 품종이 육성되었으며 (Marie, 1955), 수박 (Jaskani et al, 2005), 바 나나 (Duren et al, 1996) (Ochatt, 2008 ;Ono & Hosaka, 2010). FC를 이용할 경우 감자에서 는 95%의 확률로 배수체 확인이 가능하고 (Ono & Hosaka, 2010), 수박에서도 4배체의 확인이 가능할 뿐만 아니라 엽 면적, 엽록체 수, 공변세포 수, DNA 지수, 염색체 수 등으 로 배수체를 확인하는 것에 비해 빠르고 편리함이 보고되었 다 (Jaskani et al, 2005).…”

Perspectives on the application of biotechnology to assist the genetic enhancement of plantain and banana (Musa spp.)

“…This has led to the development of flow cytometric techniques for rapid routine ploidy determination in Musa (Dolezel et al 1994;van Duren et al 1996). However, this approach can not generally be used for more detailed analysis.…”