INDUCTION AND TERMINATION OF PUPAL DIAPAUSE IN<i>SARCOPHAGA</i>(DIPTERA: SARCOPHAGIDAE)

Denlinger, David L.

doi:10.2307/1540242

Cited by 249 publications

(97 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The first AKH was isolated from the locust, Locusta migratoria (Stone et al, 1976) Denlinger (1972). Only female flies of 4 days old were 111 used for the experiments.…”

Section: Cover Lettermentioning

confidence: 99%

Differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly, Sarcophaga crassipalpis

Bil

Broeckx

Landuyt

et al. 2014

General and Comparative Endocrinology

View full text Add to dashboard Cite

Females of anautogenous flesh flies, Sarcophaga crassipalpis, need a protein meal in order to produce their first batch of eggs. This protein meal elicits an increase in midgut proteolytic activity that is under neuropeptidergic regulation. Time series of decapitation and rescue experiments of liver fed flies evidenced the need of a peptide factor released by corpora cardiaca (CC) within four hours post protein feeding in order to assure complete protein digestion. Q-Exactive quantitative differential peptidomics analysis on CC of sugar fed flies and flies five hours post protein feeding respectively, showed a unique consistent decrease in the stored amount of adipokinetic hormone (AKH) ranging between 16 up to 63%. Injection of AKH into liver fed decapitated flies as well as sugar fed intact flies resulted in dose dependent enhanced midgut proteolytic activity up to the level of intact protein fed flies. This suggests a key role of AKH in food depended reproduction According to the reviewers minor remarks and suggestions and in response to your directions we made the appropriate changes as explained below:For Reviewer 1 the manuscript was acceptable as such and no action needed to be taken.As reviewer 2 felt uncomfortable with some of our figures as it was hard to understand each figure due to complicated representation on different food sources and time-courses, we adopted the graphical figures by incorporating line type explanation directly on the figures. In all experimental figures ( fig 3 and beyond) the broken line represent the non decapitated liver fed control situation whereas the dotted line represent the liver primed decapitated control condition and the solid line represent the liver primed decapitated experimental condition. According to directions we saved each figure file in .tiff format.As in Line 336-337 reviewer 2 asked to explain "a non-protein free amino acids" for readers. We replaced the sentence by "a free amino acids meal devoid of any protein" in line 337.3. As reviewer 2 informed about what kind of unit that was employed for enzymatic activity we adopted each figure as the relative enzymatic activity is expressed in absorption units as measured at 405 nm and normalized against the mass of the sample. Accordingly we explicitly clarified this in line 128 of M&M. We included (A 405nm ) in the figures ordinate We thank the reviewers for their suggestions and sincerely hope that the proposed changes suffice to make this manuscript ready for publication. Kind regards Prof.Dr.Roger Huybrechts Cover LetterResponse to reviews According to the reviewers minor remarks and suggestions and in response to your directions we made the appropriate changes as explained below:For Reviewer 1 the manuscript was acceptable as such and no action needed to be taken.As reviewer 2 felt uncomfortable with some of our figures as it was hard to understand each figure due to complicated representation on different food sources and time-courses, we adopted the graphical figures by incorporating line type e...

show abstract

“…The first AKH was isolated from the locust, Locusta migratoria (Stone et al, 1976) Denlinger (1972). Only female flies of 4 days old were 111 used for the experiments.…”

Section: Cover Lettermentioning

confidence: 99%

Differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly, Sarcophaga crassipalpis

Bil

Broeckx

Landuyt

et al. 2014

General and Comparative Endocrinology

View full text Add to dashboard Cite

show abstract

“…Flies were obtained from an established laboratory colony of the flesh fly, S. crassipalpis Macquart, reared under nondiapausing conditions of long day length (15:9; light/dark) at 25°C. Diapausing pupae were obtained by raising adults of the parental generation at short day length (12:12; light/dark) at 25°C and larvae at 12:12 light/dark at 20°C, as described (63). When direct comparisons were made between nondiapausing and diapausing flies, both groups were raised as larvae at 20°C while maintaining their respective light regimes.…”

Section: Methodsmentioning

confidence: 99%

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Rinehart

Yocum

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

476

409

View full text Add to dashboard Cite

Diapause, the dormancy common to overwintering insects, evokes a unique pattern of gene expression. In the flesh fly, most, but not all, of the fly's heat shock proteins (Hsps) are up-regulated. The diapause up-regulated Hsps include two members of the Hsp70 family, one member of the Hsp60 family (TCP-1), at least four members of the small Hsp family, and a small Hsp pseudogene. Expression of an Hsp70 cognate, Hsc70, is uninfluenced by diapause, and Hsp90 is actually down-regulated during diapause, thus diapause differs from common stress responses that elicit synchronous up-regulation of all Hsps. Up-regulation of the Hsps begins at the onset of diapause, persists throughout the overwintering period, and ceases within hours after the fly receives the signal to reinitiate development. The up-regulation of Hsps appears to be common to diapause in species representing diverse insect orders including Diptera, Lepidoptera, Coleoptera, and Hymenoptera as well as in diapauses that occur in different developmental stages (embryo, larva, pupa, adult). Suppressing expression of Hsp23 and Hsp70 in flies by using RNAi did not alter the decision to enter diapause or the duration of diapause, but it had a profound effect on the pupa's ability to survive low temperatures. We thus propose that up-regulation of Hsps during diapause is a major factor contributing to cold-hardiness of overwintering insects.cold tolerance ͉ overwintering ͉ stress proteins ͉ RNAi

show abstract

“…70 (=S. argyrostoma) enter pupal diapause before winter (Denlinger, 1972;Fraenkel and Hsiao, 1968;Saunders, 1971;Ohtaki and Takahashj, 1972;Vinogradova, 1976 (Denlinger, 1981;Denlinger et al 1988;Kurahashi and Ohtaki, 1989 (Denlinger, 1991 …”

mentioning

confidence: 99%

Chilling period for effective survival of diapausing pupae of the flesh fly, Boettcherisca peregrina, collected from Tokyo

Moribayashi¹,

Wells²,

Kurahashi³

1999

Med. Entomol. Zool.

View full text Add to dashboard Cite

INDUCTION AND TERMINATION OF PUPAL DIAPAUSE INSARCOPHAGA(DIPTERA: SARCOPHAGIDAE)

Cited by 249 publications

References 17 publications

Differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly, Sarcophaga crassipalpis

Differential peptidomics highlights adipokinetic hormone as key player in regulating digestion in anautogenous flesh fly, Sarcophaga crassipalpis

Up-regulation of heat shock proteins is essential for cold survival during insect diapause

Chilling period for effective survival of diapausing pupae of the flesh fly, Boettcherisca peregrina, collected from Tokyo

Contact Info

Product

Resources

About