Induction and Repair of DNA Strand Breaks in Human Cell-lines with Different Radiosensitivity

Nilsson, Sverker; Johansson, Leif

doi:10.1080/09553008114550121

Cited by 2 publications

(2 citation statements)

References 14 publications

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“…Twentyfive rnin was also obtained for endothelial cells in the rat brain irradiated with 4.5 Gy gamma rays from 6oCo (13). The shorter time, around 13 min, has earlier been reported for lymphoma cells and glioma cells irradiated in vitro with 2.8 or 8 Gy (19).…”

Section: Resultsmentioning

confidence: 53%

DNA Damage in Human Endothelial Cells after Irradiation in Anoxia

Rosander

Zackrisson

1995

Acta Oncologica

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Endothelial cells and fibroblasts have been reported to respond differently to oxidative stress. Both the effects of high oxygen tension and radiation involve the action of free radicals. DNA damage (single strand breaks, SSB, and double strand breaks, DSB) was assayed in human umbilical cord vein (HUV) cells and in Chinese hamster fibroblasts (V79) after irradiation under oxic or anoxic conditions. The cells were exposed to single doses in the range of 2-18 Gy of gamma-radiation from 60Co. Significantly more DNA damage was induced in the V79 cells than in the HUV cells. As a consequence, a higher oxygen enhancement ratio was obtained for the HUV cells (6.3) as compared to the V79 cells (2.8). The repair of SSB was slower in the HUV cells than in the V79 cells, irrespective of oxic state. For the higher doses, the damage remaining at 60 min after anoxic irradiation, i.e. DSB, was only detected in the V79 cells.

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Section: Resultsmentioning

confidence: 53%

DNA Damage in Human Endothelial Cells after Irradiation in Anoxia

Rosander

Zackrisson

1995

Acta Oncologica

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“…Strand breaks constitute major lesions in cells exposed to gamma irradiation (Nilsson & Johanson, 1981;Hutchinson, 1985). DNA damage of this nature results, in part, from indiscriminate multi-site attack by OH radicals generated during water radiolysis (Eqs.…”

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confidence: 99%

Structural damage to lymphocyte nuclei by H2O2 or gamma irradiation is dependent on the mechanism of OH. radical production

Allan

Vaughan²,

Milner³

et al. 1988

Br J Cancer

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Summary Normal human lymphocytes were exposed to OH radicals produced indirectly by exposure to H202 or directly by gamma irradiation. Using a flow cytometry technique to measure changes in nucleoid size, it was found that generation of OH in each system produced a characteristic relaxation in nuclear supercoiling. Exposure of cells to H202 produced a metal-dependent step-wise relaxation in extracted nucleoids, while gamma irradiation induced a gradual dose-dependent increase in nucleoid size. The sitespecific metal-dependent changes produced in lymphocytes incubated in H202 should also occur in gamma irradiated cells, but the characteristic effects on nuclear supercoiling would not be detected within the background of random DNA damage. The importance of metals in maintaining the supercoiled loop configuration of DNA within the protein matrix suggests that free radical damage at metal locations may be particularly toxic for the cell.

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Induction and Repair of DNA Strand Breaks in Human Cell-lines with Different Radiosensitivity

Cited by 2 publications

References 14 publications

DNA Damage in Human Endothelial Cells after Irradiation in Anoxia

DNA Damage in Human Endothelial Cells after Irradiation in Anoxia

Structural damage to lymphocyte nuclei by H2O2 or gamma irradiation is dependent on the mechanism of OH. radical production

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