Induction and partial characterization of California halibut (Paralichthys californicus) vitellogenin

Palumbo, Amanda J.; Linares-Casenave, Javier; Jewell, William T.; Doroshov, Serge I.; Tjeerdema, Ronald S.

doi:10.1016/j.cbpa.2006.10.023

Cited by 21 publications

(17 citation statements)

References 36 publications

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“…For this reason, these proteins have been deeply studied for their role in fish reproduction (De Vlaming et al, 1980;Tyler & Sumpter, 1990;Mañanós et al, 1994;Hiramatsu et al, 2002;Palumbo et al, 2007), and as biomarkers for endocrine disruption (Tyler et al, 2002;Aruwke & A. Goksøyr, 2003;Hiramatsu et al, 2006;Geraudie et al, 2017). Male adult fish and juveniles have the potential to produce Vtgs, but physiologically they do not have enough estrogens to trigger gene expression.…”

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confidence: 99%

“…This makes a necessary more in-depth study of Vtg for every species of fish. Even though Vtgs has been widely studied in different bony fish species (Hara & Hirai, 1978;De Vlaming et al, 1980;Mañanós et al, 1994;Palumbo et al, 2007;Zhong et al, 2014), in South American freshwater fish species it has been only carried out in Arapaima gigas and Rhamdia quelen (Chu-Koo et al, 2008;Mour-Costa et al, 2016). Proteomics tools such as two-dimensional gel electrophoresis and mass spectrometry have been shown to be very helpful to identify and characterize different Vtgs from plasma samples in fish (Cohen et al, 2005(Cohen et al, , 2009Palumbo et al, 2007), avoiding long and tedious purification processes.…”

Section: __________________mentioning

confidence: 99%

“…Vitellogenins (Vtgs) are large serum glycolipophosphoproteins (≈360 KDa) synthesized in the liver by all oviparous vertebrates in response to estrogen induction during oocyte maturation Palumbo et al, 2007) and are commonly presented as…”

Section: Introductionmentioning

confidence: 99%

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Proteomic characterization of vitellogenins from three species of South American fresh water fish

Urdaneta

Camafeita

Poleo

et al. 2018

lajar

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ABSTRACT. Vitellogenins (Vtg) are glycolipophosphoproteins synthesized by oviparous vertebrates as yolk proteins precursors. These proteins have been studied for their role in reproduction and in endocrine disruption. In this work, we report the first proteomic study towards the characterization of Vtg from Pseudoplatystoma fasciatum, Piaractus brachypomus and Colossoma macropomum. Male specimens of each of the three fish species were estradiol-induced (experimental) and non-induced (control). The initial Vtg characterization was made by 2D protein gel electrophoresis of both groups The identification of the high molecular weight spots, presumed to be Vtgs, was assessed by MALDI-TOF mass spectrometry analysis. A post-translational modification study was performed by differential staining of 2D gels in order to visualize phosphoproteins and glycoproteins. Plasma samples from the three species, induced with estrogen, showed three high molecular weight spots with variable isoelectric points. Post-translational modifications showed that Vtgs from P. brachypomus and C. macropomum presented a phosphorylated and glycosylated subunit, while the same subunit in P. fasciatum was only glycosylated. This characterization will help in the development of reliable immunoassays, which could be used for studies of endocrine disruption or for the improvement of artificial spawning, by uncovering the time of fish maturation or sex determination.

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confidence: 99%

Section: __________________mentioning

confidence: 99%

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Proteomic characterization of vitellogenins from three species of South American fresh water fish

Urdaneta

Camafeita

Poleo

et al. 2018

lajar

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“…Vitellogenin was purified using a modification of the method developed by Palumbo et al [15]. Plasma from estrogen-treated males (induction) was pooled and used as source of vitellogenin.…”

Section: Purification Of Vitellogeninmentioning

confidence: 99%

Use of Vitellogenin as Biomarker Indicator in Sex Identification of Giant Grouper (Epinephelus lanceolatus)

A¹,

Jasmani²,

Yy³

et al. 2016

Poult Fish Wildl Sci

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“…Abundant literature exists on broodstock management and culture technology of other flatfishes of the genus Paralichthys such as P. olivaceus in Japan (Alam et al, 2002;Furuita et al, 2002;Hernández et al, 2007;Yamaguchi et al, 2007), P. microps in Chile (Silva, 2001), P. californicus (Conklin et al, 2003;Gisbert et al, 2004;Merino et al, 2007;Palumbo et al, 2007) and P. lethostigma (Smith et al, 1999;Watanabe & Carroll, 1999) in the United States, P. orbignyanus in Argentina and Brazil Müller et al, 2006;Lanes et al, 2009Lanes et al, , 2010; although little is known about the use of Recirculating Aquaculture System (RAS) technology for broodstock culture of P. adspersus.…”

Section: Introductionmentioning

confidence: 99%

Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867) using recirculating aquaculture systems

Carrera

Cota

Montes

et al. 2013

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ABSTRACT. The present study describes the methodology used at IMARPE for the capture, acclimation and management of P. adspersus broodstock using recirculating aquaculture systems (RAS). RAS improved the water quality and maintained the environmental parameters during the acclimation period, temperature (17.2±1ºC), oxygen (8.1±0.7 mg L -1 ), pH (7.3±0.2), ammonia (0.004±0.003 mg L -1 ), nitrite (0.52±0.2 mg L -1 ) and nitrate (3.45±2.6 mg L -1 ). Fish began to be fed normally from day 15 post-capture, once or twice a day using live fish (Odonthestes regia regia, Mugil cephalus), crustacean (Emerita analoga), fresh food (Engraulis ringens and Dosidicus gigas) and artificial feed. A significant loss in the weight of the fish was registered during the first days of captivity, followed by a continuous increase in both sexes. The specific growth rate was positive from the third month of captivity, being the relative growth rate 24.5% and 16.2% in August 2010 in males and females, respectively. Different internal and external parasites were detected in the fish, being Entobdella sp. and Philometra sp. the prevailing parasites observed during samplings. Keywords: Paralichthys adspersus, reproduction, recirculating aquaculture system, broodstock management, culture, Peru. Manejo de reproductores del lenguado Paralichthys adspersus (Steindachner, 1867)usando sistemas de recirculación en acuicultura RESUMEN. El presente trabajo, describe la metodología desarrollada en IMARPE para la captura, aclimatación y acondicionamiento de ejemplares adultos de P. adspersus en sistemas de recirculación (SRA), con la finalidad de formar un stock de reproductores. El SRA permitió manejar parámetros medioambientales estables durante el periodo de acondicionamiento, como: temperatura del agua (17,2±1ºC), oxígeno disuelto (8,1±0,7 mg L -1 ), pH (7,3±0,2), amonio (0,004±0,003 mg L -1 ), nitrito (0,52±0,2 mg L -1 ) y nitrato (3,45±2,6 mg L -1 ). Se dio inicio a la alimentación el día 15 post-captura, utilizando alimento vivo (Odonthestes regia regia, Mugil cephalus), crustáceos (Emerita analoga), alimento fresco (Engraulis ringens y Dosidicus gigas) y artificial semihúmedo. Durante los primeros días de acondicionamiento los peces mostraron una disminución en el peso, hasta su adaptación a las condiciones de cultivo, luego de lo cual se produjo un incremento continuo en ambos sexos. La tasa específica de crecimiento fue positiva a partir del tercer mes y la tasa de crecimiento relativo mostró que en agosto 2010, el peso promedio se incrementó 24,5% en machos y 16,2% en hembras. Se realizó un análisis patológico a los ejemplares capturados y se observó la presencia de diferentes parásitos internos y externos, entre ellos predominaron Entobdella sp. y Philometra sp. Latin American Journal of Aquatic ResearchPalabras clave: Paralichthys adspersus, reproducción, sistema de recirculación en acuicultura, manejo de reproductores, cultivo, Perú.

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Induction and partial characterization of California halibut (Paralichthys californicus) vitellogenin

Cited by 21 publications

References 36 publications

Proteomic characterization of vitellogenins from three species of South American fresh water fish

Proteomic characterization of vitellogenins from three species of South American fresh water fish

Use of Vitellogenin as Biomarker Indicator in Sex Identification of Giant Grouper (Epinephelus lanceolatus)

Broodstock management of the fine flounder Paralichthys adspersus (Steindachner, 1867) using recirculating aquaculture systems

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