1976
DOI: 10.1016/0042-6822(76)90251-8
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Induction and isolation of mutants in a specific region of gene A of bacteriophage ∅X174

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Cited by 21 publications
(10 citation statements)
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“…Weissmann and his colleagues (26) described a site-directed mutagenesis procedure that uses coliphage Qfl replicase to incorporate nucleotide analogs at specific sites in Q(3 RNA, and Borrias et al (27) isolated temperature-sensitive mutants of coliphage 4X174 after transfection with a partial heteroduplex consisting of singlestranded phage DNA and a mutagenized fragment. With suitable modifications, each of these approaches might have more general applicability.…”
Section: Methodsmentioning
confidence: 99%
“…Weissmann and his colleagues (26) described a site-directed mutagenesis procedure that uses coliphage Qfl replicase to incorporate nucleotide analogs at specific sites in Q(3 RNA, and Borrias et al (27) isolated temperature-sensitive mutants of coliphage 4X174 after transfection with a partial heteroduplex consisting of singlestranded phage DNA and a mutagenized fragment. With suitable modifications, each of these approaches might have more general applicability.…”
Section: Methodsmentioning
confidence: 99%
“…In this approach, deletions or insertions can be introduced rather easily. However, methods to generate point mutations at predetermined sites in a DNA molecule by base-specific chemical treatment (2)(3)(4)(5) or by incorporation of nucleotide analogs (1) give rise to only a few mutants among a large background ofnonmutants. Because this yield is too low to permit saturation of a genetic element with point mutations, we developed an improved method for sodium bisulfite mutagenesis in vitro.…”
mentioning
confidence: 99%
“…The local mutagenesis methods as well as other methods for site-directed mutagenesis (Borrias et al 1976;Weissmann et al, this volume)should be increasingly useful for modifying specific nucleotides in genes and regulatory signals of viral or other cloned DNA segments. The availability of restriction maps and extensive nucleotide sequences should make it possible to select functionally interesting sites for systematic perturbation, whether in coding or in regulatory sequences.…”
Section: Discussionmentioning
confidence: 98%