Induction and expression of cyclin D3 in human pancreatic cancer

Ebert, Matthias P.; Hernberg, Sara; Guo, Fei; Sokołowski, A.; Schulz, H.-U.; Lippert, H.; Malfertheiner, Peter

doi:10.1007/s004320100235

Cited by 80 publications

(104 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Total RNA was extracted from the human pancreatic tissues and the pancreas of the transgenic mice by the acid guanidinium-thiocyanate method, as previously described (Ebert et al, 1994).…”

Section: Rna Extractionmentioning

confidence: 99%

“…PCR amplification was performed after an initial denaturation of 3 min at 948C, followed by 40 cycles of 45 s at 948C, 45 s at 548C and 1 min at 728C, and finally 10 min of final elongation at 728C. The PCR products were treated with NsiI at 378C for 2 h and then separated on 1.5% agarose gel (Ebert et al, 1994). The level of PTEN mRNA was analyzed densitometrically from the agarose gel and was standardized to the respective enolase or GAP mRNA level.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…The analysis was performed according to the manufacturer's recommendations and all reactions were performed at 238C. Finally, the sections were counterstained with Mayer's hematoxylin (Ebert et al, 1994).…”

Section: Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1

et al. 2002

View full text Add to dashboard Cite

PTEN is a candidate tumour suppressor gene and frequently mutated in multiple cancers, however, not in pancreatic cancer.Recently, it has been demonstrated that PTEN expression is regulated by TGF-b1. Using TGF-b1 transgenic mice (n=7) and wildtype littermates (n=6), as well as pancreatic tissues obtained from organ donors (n=10) and patients with pancreatic cancer (n=10), we assessed the expression of PTEN by means of immunohistochemistry and semiquantitative PCR analysis. In addition, PANC-1 cells were treated with TGF-b1 in vitro and the levels of PTEN mRNA were determined in these cells. In human pancreatic cancers PTEN mRNA levels were significantly decreased (P50.05). In addition, in the pancreas of TGF-b1 transgenic mice the expression of PTEN was significantly reduced (P50.01), as compared to wildtype littermates and incubation of PANC-1 cells with TGF-b1 decreased PTEN mRNA levels after 24 h. Inasmuch as TGF-b1 decreases PTEN expression in human pancreatic cancer cells and human pancreatic cancers overexpress TGF-b1, the reduced expression of PTEN in pancreatic cancer may be mediated by TGF-b1 overexpression. Thus, although PTEN is not mutated in pancreatic cancers, the reduction of its expression may give pancreatic cancer cells an additional growth advantage.

show abstract

“…Total RNA was extracted from the human pancreatic tissues and the pancreas of the transgenic mice by the acid guanidinium-thiocyanate method, as previously described (Ebert et al, 1994).…”

Section: Rna Extractionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Indeed, overexpression of the D-type cyclins has been reported in a large number of tumours (Sicinski et al, 1996;Zhang, 1999;Zhang and Li, 1999;Florenes et al, 2000;Ebert et al, 2001;Ito et al, 2001;Wong et al, 2001). However, not all three D-type cyclins are overexpressed in the same tumour and the three cyclins are differentially linked to poor outcome of the disease.…”

mentioning

confidence: 98%

Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas

et al. 2005

View full text Add to dashboard Cite

Cyclins D1, D2 and D3 play important roles in cell proliferation and differentiation. Although their abnormal expression has been linked to cancer development and progression in a number of tissues, the expression of cyclin D2 and D3 proteins in colon cancer has not yet been characterised. In this study, we examined cyclin D1, D2 and D3 protein expression by Western blot analysis in tumour and adjacent normal colon tissues of 57 patients. In addition, we examined D-type cyclins protein expression in HT29 and LoVo39 cell lines from colon carcinomas, as a function of induced proliferation and differentiation. In both cell lines, the expression of the three D-type cyclins increased as a result of induced proliferation, whereas the expression of cyclin D3 increased as a result of induced differentiation. In colon tumours, cyclin D1 was overexpressed in 44%, cyclin D2 was overexpressed in 53% and cyclin D3 was overexpressed in 35% of the cases. We also found that in 16% of the cases, cyclin D3 protein expression was reduced in the tumour, as compared to the adjacent normal tissue. Examination of D-type cyclin protein overexpression in relation to the TNM stage of the tumours revealed that overexpression of cyclins D1 and/or D2, but not cyclin D3, is linked to colon carcinogenesis and that overexpression of cyclin D2 may be related to a higher TNM stage of the tumour.

show abstract

“…Nonetheless, there are reports of increased expression of AR, BTC, HB-EGF and EPR in PDAC. [38][39][40][41] For AR and for HB-EGF, correlation with advanced disease and poor prognosis has been documented. 38,41 Cell culture experiments with PCCL have shown increased cancer cell growth under stimulation with each of those ligands.…”

mentioning

confidence: 99%

Expression and differential signaling of heregulins in pancreatic cancer cells

Kolb

Kleeff

Arnold

et al. 2006

Intl Journal of Cancer

View full text Add to dashboard Cite

The EGF family of ligands and receptors plays an important role in the pathogenesis of pancreatic ductal adenocarcinoma (PDAC) and contributes to its aggressiveness. A number of molecular approaches have been developed to block these pathways, and studies have already proven the clinical benefit of this concept in PDAC. In the present study, we sought to determine the potential role of heregulins (HRGs), a family of EGF-like growth factors, in PDAC. Quantitative RT-PCR analysis revealed that HRGs as well as its signaling ErbB receptors were present in 4 of 4 human pancreatic cancer cell lines (PCCL). HRG-b1 stimulated the growth of 3 of 4 PCCL, whereas HRG-a1 inhibited cell growth in 3 of 4 cell lines. Responses towards HRGs could in part be predicted by ErbB2 and ErbB3 expression levels. HRGs induced phosphorylation of different ErbB receptors as well as activation of MAPK, p38MAPK, JNK and PI3K in a cell-and ligand-specific manner. In vivo, HRG was upregulated in pancreatic cancer tissues and localized predominantly in the cancer cells. High HRG-b levels but not HRG-a levels were associated with decreased patient survival. In conclusion, HRG is expressed by pancreatic cancer cells and influences pancreatic cancer cell growth and patient survival. ' 2006 Wiley-Liss, Inc.

show abstract

Induction and expression of cyclin D3 in human pancreatic cancer

Cited by 80 publications

References 10 publications

Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1

Reduced PTEN expression in the pancreas overexpressing transforming growth factor-beta 1

Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas

Expression and differential signaling of heregulins in pancreatic cancer cells

Contact Info

Product

Resources

About