Induction and characterization of cutaneous lymphocyte antigen on natural killer cells

Abstract

Summary. Cutaneous lymphocyte antigen (CLA) has been reported to be expressed mainly by memory/effector T lymphocytes infiltrating inflammatory skin lesions and cutaneous T-cell lymphoma. It has been suggested that CLA is a specific homing receptor, facilitating the T-cell migration into skin lesions, and also an indicator of the skinhoming T-cell subset. In the present study, we investigated the expression of CLA in natural killer (NK) cells defined phenotypically as surface CD3 -and CD56 + cells in peripheral blood. CLA was definitely expressed on CD3 -CD56 + cells at a level comparable to CD3 + cells in peripheral blood of normal Japanese volunteers. After in vitro stimulation of peripheral blood mononuclear cells with interleukin 2 (IL-2) and IL-12, there was a significant increase in the number and percentage of CLA + NK cells but not CLA + T cells (P < 0AE01). To analyse the characteristics of CLA expressed by NK cells, we investigated a CLA + NK-leukaemia cell line, NK-YS, established from a patient with NK leukaemia/ lymphoma with skin infiltration. In the in vitro study, the CLA-expressing NK-leukaemic cell line bound to E-selectintransfected cells and was inhibited by HECA 452 antibody or neuraminidase treatment of leukaemic cells. These findings suggest that CLA expressed by NK cells is a homing receptor for the E-selectin molecule and may explain skin infiltration by NK cells and NK lymphoma cells analogous to T cells. An NK-cell subset expressing CLA must play an important role in host defence against microorganisms and neoplasms in skin lesions.

Optimized immunohistochemical panel to differentiate myeloid sarcoma from blastic plasmacytoid dendritic cell neoplasm

“…22 Petrella et al 7 studied CLA/CD162 and found it to be positive in 90% of BPDCN and 78% of MS cases, suggesting the lack of specificity of this marker in distinguishing MS and BPDCN. In contrast, CLA/CD162 was positive in 3/9 (33%) BPDCN cases and in 0/11 (0%) cases of MS in our study.…”

Cutaneous lymphocyte antigen-positive T cells may predict the development of acute GVHD: alterations and differences of CLA+ T- and NK-cell fractions

“…Stacey et al 28 showed that the pretreatment of donor lymphocytes with neuraminidase decreases the incidence of aGVHD in murine models. This study is indirect evidence suggesting the involvement of the murine counterpart of CLA in the systemic aGVHD reaction as CLA is a sialylated 12,14 It was also shown that Th1 T cells expressing skin homing receptors can migrate to the intestinal laminapropria, and are associated with inflammation in the intestine. 29 Further, the CLA þ T cells that emerge after allo-SCT express high levels of CCR4 and CCR6, which is comparable to that of the normal donors.…”

“…A total of 1 Â 10 7 PBMCs obtained from normal volunteers were cultured and stimulated with 1 mg/ml PHA (Sigma), 100 U/ml rIL-2 (Shionogi and Co. Ltd., Osaka, Japan) and 50 ng/ml rIL-12, (Pepro Tech Inc., Rocky Hill, NJ, USA) for 5 days as reported previously. 14,15 The whole nonadherent cells were collected for analysis of CLA expression using a FACScan flow cytometer. Activated cells increased their size and the Surface-marker analysis of CLA þ T cells after allo-SCT To determine the characteristics of CLA þ T cells in PB, we obtained lymphocytes from the normal donors (n ¼ 6) and the patients at 2-4 (n ¼ 10) and 12-14 weeks (n ¼ 7), after allo-SCT.…”

“…8,9 Among them, the cutaneous lymphocyte antigen (CLA)-a sialylated P-selectin glycoprotein ligand-1-mediates the specific skin homing of T or natural killer (NK) cells circulating in the peripheral blood (PB). [10][11][12][13][14] The microenvironment of skin or skin-associated lymph node that is necessary for CLA expression has not been fully clarified. However, in in vitro experiments, the CLA expression on T cells was specifically induced by stimulation with IL-2, IL-12 and phytohemagglutinin (PHA, antigen signal), whereas that on NK cells was induced by stimulation with IL-2 and IL-12.…”

Leucémie aiguë CD4+/CD56+: à propos d’un cas clinique au service d’hématologie CHU Purpan, Toulouse (France)

“…L'aspect histologique n'est pas spé cifique de la maladie. La prolifé ration est composé e de cellules de taille moyenne, assez monomorphes, et le diagnostic é voqué est celui d'un lymphome B ou T, d'une localisation cutané e d'une leucé mie myé loïde ou d'un sarcome granulomateux [8,13]. La cytochimie retrouve toujours les MPO et esté -rases né gatives [3].…”