Induction and Accumulation of Major Tuber Proteins of Potato in Stems and Petioles

Paiva, Edílson; Lister, R. M.; Park, William D.

doi:10.1104/pp.71.1.161

Cited by 154 publications

(112 citation statements)

References 10 publications

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“…A similar phenomenon to that discussed herein has been reported previously in potato plants by Paiva et al. (21), who observed that the accumulation of large amounts of the tuber storage protein, patatin, could be induced in stems and petioles of single-leaf stem cuttings concomitant with the accumulation of large amounts of other major tuber proteins, as well as starch. Results from several laboratories indicate that the expression of class I patatin genes of potato is also regulated by sucrose and that induction of these genes by sucrose accompanies the accumulation of starch (10,24,29).…”

Section: Effects Of Various Sugars On Inductionsupporting

confidence: 64%

Sucrose-Induced Accumulation of β-Amylase Occurs Concomitant with the Accumulation of Starch and Sporamin in Leaf-Petiole Cuttings of Sweet Potato

Nakamura¹,

Ohto²,

Yoshida³

et al. 1991

Plant Physiol.

105

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ABSTRACT,8-Amylase of sweet potato (Ipomoea batatas L.), which constitutes about 5% of the total soluble protein of the tuberous root, is absent or is present in only small amounts in organs other than the tuberous roots of the normal, field-grown plants. However, when leaf-petiole cuttings from such plants were supplied with a solution that contained sucrose, the accumulation of 8-amylase was induced in both leaf and petiole portions of the explants. The sucrose-induced accumulation of fl-amylase in leaf-petiole cuttings occurred concomitant with the accumulation of starch and of sporamin, the most abundant storage protein of the tuberous root. The accumulation of fl-amylase, of sporamin and of starch in the petioles showed similar dependence on the concentration of sucrose, and a 6% solution of sucrose gave the highest levels of induction when assayed after 7 days of treatment. The induction of mRNAs for f-amylase and sporamin in the petiole could be detected after 6 hours of treatment with sucrose, and the accumulation of j6-amylase and sporamin polypeptides, as well as that of starch, continued for a further 3 weeks. In addition to sucrose, glucose or fructose, but not mannitol or sorbitol, also induced the accumulation of fl-amylase and sporamin, suggesting that metabolic effects of sucrose are important in the mechanism of this induction. Treatment of leaf-petiole cuttings with water under continuous light, but not in darkness, also caused the accumulation of small amounts of these components in the petioles, probably as a result of the endogenous supply of sucrose by photosynthesis. These results suggest that the expression of the gene for f-amylase is under metabolic control which is coupled with the expression of sink function of cells in the sweet -otato.

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Section: Effects Of Various Sugars On Inductionsupporting

confidence: 64%

Sucrose-Induced Accumulation of β-Amylase Occurs Concomitant with the Accumulation of Starch and Sporamin in Leaf-Petiole Cuttings of Sweet Potato

Nakamura¹,

Ohto²,

Yoshida³

et al. 1991

Plant Physiol.

105

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“…Combining these results with those from whole-plant morphology (3) would demonstrate conclusively that the Etuberosa types lack the capacity to form either stolons or tubers. By manipulating sink availability, however, the tuber proteins can be induced to accumulate without tuber formation along with large amounts of starch, in stems and petioles from a petiole-leaf cutting system (10). These petiole-leaf cuttings with the axillary bud removed can be used as a system for inducing expression of tuber protein genes in the absence of tuber morphology.…”

Section: Resultsmentioning

confidence: 99%

“…Because of the differences in transcript accumulation, it seems that conditions exist in the S. brevidens x S. chacoense hybrid to differentially modify the pattern of regulation during the expression of at least one abundant tuber protein in induced petiole tissue. Normally, the accumulation of these two tuber proteins (patatin and the 22-kD type represented by p740) occurs coordinately in both developing tubers and induced petioles (10).…”

Section: Resultsmentioning

confidence: 99%

Differential Expression of Potato Tuber Protein Genes

Hannapel¹

1990

Plant Physiol.

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Patatin and the 22-kilodalton protein complex make up more than 50% of the soluble protein present in potato (Solanum tuberosum) tubers and these two proteins are coordinately regulated during tuber development. Although genomic sequences related to these tuber genes exist in the genome of potato species that do not bear tubers, they cannot be induced into expression under the tested conditions. These genes are not expressed during substantial starch accumulation in petioles from a model petiole-leaf cuffing system in nontuber-beanng plants, indicating that starch accumulation and synthesis of the major tuber proteins occur independently. Tuber protein gene expression also has been examined in hybrid potato plants that contain genomes from both tuberizing and nontuberizing species. One such triploid hybrid produced only stolons, whereas a pentaploid hybrid with an increased number of tuber genomes produced tubers. It was shown, using immunoblotting and Northem blot hybridization, that these two hybrids actively expressed both patatin and the 22-kilodalton tuber protein in induced petioles from the leaf-cutting system. The induced accumulation of patatin transcripts was consistent in all genotypes containing some tuberizing genome. The induced accumulation of the 22-kilodalton protein transcripts, however, was lower in genotypes containing some nontuberizing genome. Sucrose induction of these genes in leaves corroborates the induction patterns in petioles. A correlation exists between 22-kilodalton protein gene expression and a potato plant's ability to produce stolons or tubers. Even though patatin and the other major tuber proteins do not exhibit strict tuber-specific expression, their very close correlation with the early events of tuber development and their relative abundance in tubers distinguish them as possible markers for the ongoing processes oftuberization. It is possible that substantial tuber protein accumulation in nontuber tissue reflects the induction of specific biochemical events of tuberization without the accustomed morphological response. To ascertain if such biochemical induction is possible in potato species incapable of attaining tuber morphology, this report focuses on the induced expression oftwo distinct potato-tuber genes present in genomes of plants that do not bear tubers.Diploid species of the series Etuberosa do not bear tubers and have been identified by geneticists as a reservoir of germ plasm of potential value for breeding for disease resistance, quality, and environmental adaptation (6). In an attempt to transfer nontuber potato germ plasm to Solanum tuberosum, Ehlenfeldt and Hanneman (3) identified a triploid hybrid (2n = 3x = 36), which produced only stolons, and a pentaploid hybrid (2n = 5x = 60), which produced tubers. The triploid hybrid (BC) resulted from a cross between a colchicine-induced Solanum brevidens (a diploid species without tubers) and S. chacoense (a diploid tuber-bearing species). The pentaploid hybrid (BCT) resulted from a cross between the triploi...

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“…Differents auteurs ont signale ! 'accumulation de patatine, ainsi que d'amidon, en particulier dans les tiges et les petioles d'explants de pomme de terre depourvus de bourgeons (Paiva et al, 1983) ou qui ne tuberisent pas (Lavieville, 1991 ).…”

Section: Discussion Et Conclusionunclassified

Applications d'un modèle de développement et de tubérisation synchrones chez la Pomme de terre cultivéein vitro. Evolution d'activités enzymatiques

Charles¹,

Rossignol

1995

Acta Botanica Gallica

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Resume.-Dans cette etude a ete applique un modele de developpement et de tuberisation synchrones, obtenu a partir d'explants uninodaux chez Ia Pomme de terre cultivee in vitro, en absence de tout regulateur de croissance. Les parties superieures et basales des tiges de ces plantes, prelevees au cours du temps, ont ete dosees pour leur teneur en proteines solubles et leurs activites invertase et saccharose synthase. L'activite invertase, utilisee comme marqueur de l'activite metabolique de Ia plante, est nettement accrue dans Ia partie superieure des tiges chez les plantes induites en jours courts, particulierement avec thermoperiode. Cette activite importante peut traduire une amplification du metabolisme des sucres, que nous avons associee a une etape de preinduction de Ia plante. L'activite saccharose synthase s'exprime de fa<;on ponctuelle au niveau des tubercules en cours de remplissage.Summary.-In this study was used a model of synchronous development and tuberization, obtained in vitro from uninodal stem fragments of potato, without adding any exogenous growth regulator. Upper and basal parts of the stems were sampled at different times and analysed for their soluble protein content and for their invertase and sucrose synthase activities. Invertase activity, used as an indicator of the metabolic activity, increased dramatically in the upper part of the stems of plants induced by short days, particularly with a thermoperiod. This high activity could point out an increase of the sugar metabolism which was associated to a preinducting step in the plants. The sucrose synthase activity presents a peak at the tuber level during they are swelling.

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Induction and Accumulation of Major Tuber Proteins of Potato in Stems and Petioles

Cited by 154 publications

References 10 publications

Sucrose-Induced Accumulation of β-Amylase Occurs Concomitant with the Accumulation of Starch and Sporamin in Leaf-Petiole Cuttings of Sweet Potato

Sucrose-Induced Accumulation of β-Amylase Occurs Concomitant with the Accumulation of Starch and Sporamin in Leaf-Petiole Cuttings of Sweet Potato

Differential Expression of Potato Tuber Protein Genes

Applications d'un modèle de développement et de tubérisation synchrones chez la Pomme de terre cultivéein vitro. Evolution d'activités enzymatiques

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