Inducing sporulation in Alternaria solani II. Effect of light

Prasad, B. K.; Dutt, Bhupender

doi:10.1007/bf02055972

Cited by 5 publications

(2 citation statements)

References 10 publications

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“…The cultural variability among different isolates of A. solani was not prominent on PDA. Some researcher such as Kumangai and Oda(1969), Kaoru and Mitsuo (1970), Prasad and Dutt (1974), Singh(1967), Fourtouni et al (1998), Lukens (1962), Douglas and Pavek (1971) and Cotty (1987) to irradiation by u.v. light, followed by a period of darkness while Kaoru and Mitsuo (1970) reported that effect of continuous white fluorescent light (340 ml and 365 ml) exposed with short distance on culture plates of A. solani increased the spore production on three selective nutrient media i.e., V-8 juice medium, pear leaf juice medium and dry apricot V-8 juice medium.…”

Section: Sporulationmentioning

confidence: 99%

Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India

Kumar

Haldar

Pandey

et al. 2007

World J Microbiol Biotechnol

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Early blight (Alternaria solani) is an important disease causing severe damage in tomato. The eleven isolates of A. solani designated as So, Dh, Sh, Va-5, Ka, Ma, Hy, Ba-1, My, Va-3 and Mi were collected from different agroclimatic conditions and these isolates were characterized for cultural, morphological, pathogenic and molecular variations. The pigmentation varied from yellow, brown, black, brownish to greenish black in isolates of A. solani on potato dextrose agar medium. In general, radial growth of all isolates ranged between 14.9 mm and 32.2 mm on PDA and 24.3 mm to 53.7 mm on three selective media i.e., ASM, V-8 juice agar and V-8 juice agar (synthetic) on the fourth day. The fastest radial growth was recorded in the So isolate and slowest in the Ka isolate on PDA, while isolates Dh, Ba-1 and Va-3 were recorded to be faster in growth on ASM, V-8 juice agar and V-8 juice agar (synthetic) medium. The thickness of conidiogenous hyphae varied between 1.17 l and 9.56 l, with maximum in the Va-5 and Ma isolates. Most of the isolates showed smooth mycelial growth with circular and irregular margin and without concentric zonation. Sporulation was not found in any of the isolates on four different nutrient media, whereas conidiogenous hyphal length was observed in V-8 juice agar medium only. Based on the pathogenicity, isolates of A. solani were rated as virulent or less virulent based on percentage disease incidence data. Molecular variability studies were also done to find out the best annealing temperature and eighty-six primers were screened to select for maximum polymorphism of DNA. The best annealing temperature was recorded between 32.5°C and 34.0°C for the pathogen, and most efficient amplification and polymorphism of DNA was found with random primer 5 0 -CGCGTTCCTG-3 0 .

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Section: Sporulationmentioning

confidence: 99%

Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India

Kumar

Haldar

Pandey

et al. 2007

World J Microbiol Biotechnol

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“…In addition, A. solani is capable of producing extracellular polysaccharides, carbohydrases, proteases, the new zinniol-related phytotoxins [ 4 , 5 ], and other secondary metabolites during infection [ 6 , 7 ]. It has been documented that sporulation of A. solani depends upon many factors such as mycelial wounding, temperature, visible light, water treatment, ozone and ultraviolet [ 8 – 11 ]. Growth characteristics, genetic and pathogenic variations of A. solani have been studied as well [ 12 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genome sequence of the potato pathogenic fungus Alternaria solani HWC-168 reveals clues for its conidiation and virulence

Zhang

Haddadi

et al. 2018

BMC Microbiol

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BackgroundAlternaria solani is a known air-born deuteromycete fungus with a polycyclic life cycle and is the causal agent of early blight that causes significant yield losses of potato worldwide. However, the molecular mechanisms underlying the conidiation and pathogenicity remain largely unknown.ResultsWe produced a high-quality genome assembly of A. solani HWC-168 that was isolated from a major potato-producing region of Northern China, which facilitated a comprehensive gene annotation, the accurate prediction of genes encoding secreted proteins and identification of conidiation-related genes. The assembled genome of A. solani HWC-168 has a genome size 32.8 Mb and encodes 10,358 predicted genes that are highly similar with related Alternaria species including Alternaria arborescens and Alternaria brassicicola. We identified conidiation-related genes in the genome of A. solani HWC-168 by searching for sporulation-related homologues identified from Aspergillus nidulans. A total of 975 secreted protein-encoding genes, which might act as virulence factors, were identified in the genome of A. solani HWC-168. The predicted secretome of A. solani HWC-168 possesses 261 carbohydrate-active enzymes (CAZy), 119 proteins containing RxLx[EDQ] motif and 27 secreted proteins unique to A. solani.ConclusionsOur findings will facilitate the identification of conidiation- and virulence-related genes in the genome of A. solani. This will permit new insights into understanding the molecular mechanisms underlying the A. solani-potato pathosystem and will add value to the global fungal genome database.Electronic supplementary materialThe online version of this article (10.1186/s12866-018-1324-3) contains supplementary material, which is available to authorized users.

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Intracellular substrates of a heme-containing ascorbate oxidase in Pleurotus ostreatus

et al. 2009

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A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as L-ascorbic acid, D-erythroascorbic acid, 5-O-(alpha-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(alpha-D-gluco-pyranosyl)-D-erythroascorbic acid, and 5-methyl-5-O-(alpha-D-xylopyranosyl)-D-erythroascorbic acid. All of the compounds were oxidized at a significant rate by the heme-containing ascorbate oxidase. Oxidation of the compounds produced equimolar amounts of hydrogen peroxide per mole of substrate.

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Inducing sporulation in Alternaria solani II. Effect of light

Cited by 5 publications

References 10 publications

Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India

Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India

Genome sequence of the potato pathogenic fungus Alternaria solani HWC-168 reveals clues for its conidiation and virulence

Intracellular substrates of a heme-containing ascorbate oxidase in Pleurotus ostreatus

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