Inducing salt tolerance in sweet corn by magnetic priming

Abstract

This study evaluates seed germination and growth of sweet corn under NaCl stress (0, 50, and 100 mM), after exposing the seeds to weak (15 mT) or strong (150 mT) magnetic fields (MF) for different durations (0, 6, 12, and 24 hours). Salinity reduced seed germination and plant growth. MF treatments enhanced rate and percentage of germination and improved plant growth, regardless of salinity. Higher germination rate was obtained by the stronger MF, however, the seedling were more vigorous after priming with 15 mT MF. Proline accumulation was observed in parallel with the loss of plant water content under 100 mM NaCl stress. MF prevented proline accumulation by improving water absorption. Positive correlation between H 2 O 2 accumulation and membrane thermostability (MTI) was found after MF treatments, which revealed that MF primed the plant for salinity by H 2 O 2 signaling. However, over-accumulation of H 2 O 2 after prolonged MF exposure adversely affected MTI under severe salt stress. In conclusion, magnetic priming for 6 hours was suggested for enhancing germination and growth of sweet corn under salt stress. IZVLEČEK INDUKCIJA TOLERANCE NA SOL SLADKE KORUZE S PREDHODNIM OBRAVNAVANJEM SEMEN Z MAGNETNIM POLJEMV raziskavi sta bili ovrednoteni kalitev in rast sladke koruze v razmerah solnega stresa (NaCl; 0, 50, in 100 mM), po izpostavitvi semen šibkemu (15 mT) in močnemu (150 mT) magnetnemu polju (MF) v trajanju 0, 6, 12, in 24 ur. Slanost je zmanjšala kalitev semen in rast rastlin. Obravnavanje z magnetnim poljem je povečalo hitrost in odstotek kalitve ter izboljšalo rast rastlin, ne glede na slanost. Kalitev je bila značilno večja pri obravnavanju z močnejšim magnetnim poljem, kalice so bile bolj vitalne pri obravnavanju z 15 mT MF. Akumulacija prolina je bila opažena sočasno z izgubo vsebnosti vode v razmerah močnega NaCl stresa. Obravnavanje z magnetnim poljem je izboljšalo absorbcijo vode in preprečilo akumulacijo prolina. Pozitivna korelacija med akumulacijo H 2 O 2 in termostabilnostjo membran (MTI), ki je bila opažena po obravnavanju z MF je pokazala, da so bile tako obravnavane rastline odpornejše na slanost preko H 2 O 2 signalizacije. Kljub temu je prekomerna akumulacija H 2 O 2 , kot posledica podaljšane izpostavitve MF, negativno vplivala na MTI v razmerah solnega stresa. Zaključimo lahko, da predobravnavanje semen z magnetnim poljem za 6 ur pospeši nihovo kalitev in rast rastlin v razmerah solnega stresa. Ključne

Boron Enhances Antioxidative Defense in the Leaves of Salt-affected <i>Pistacia vera</i> Seedlings

“…2), revealed that the loss of cell membrane integrity was mainly due to the accumulation of H 2 O 2 in the leaves, which intensified lipid peroxidation. The level of MDA is often monitored as an indicator of oxidative destruction of plasma membranes (Karimi et al, 2017;Mittler, 2002). ELP and MDA were minimum in the moderate B level + salt stress condition (Table 2).…”

Physiological and biochemical changes in wheat cultivars under salt stress as affected by foliar boron application

“…Bitki dokularındaki serbest prolin birikiminin tuz toleransı ile ilgili olabileceği belirtilmiştir [51]. Ancak bazı araştırıcılar da bitkilerdeki serbest prolin birikiminin stres hasarının bir göstergesi olduğunu ileri sürmüşlerdir [52]. Çalışmamızda tuz stresi altındaki buğday genotiplerinde yapraklardaki serbest prolin miktarı FB uygulamaları sonucunda belirgin oranda artmıştır (Şekil 3D).…”

Priming of Silybum marianum (L.) Gaertn seeds with H2O2 and magnetic field ameliorates seawater stress

“…(2015) on S. marianum indicated that different strengths of the magnetic field and different time durations are important factors which can influence the plant growth. Karimi et al. (2017) found that magnetic priming for 6 hours was suggested for enhancing germination and growth of sweet corn under salt stress.…”

“…Proline accumulation is a widespread response of plants to environmental stresses (Anjum, 2008), which is shown to be involved in defense of plants against salinity and osmotic stress (Karimi et al., 2017). The present study revealed that soluble proline in the studied species accumulated with highly significant in response to 10% sea water stress under pretreated with H 2 O 2 and MF durations.…”

“…(2017). Electric and/or magnetic treatments are assumed to enhance seed vigor by influencing the biochemical processes that involve free radicals and by stimulating the activity of proteins and enzymes (Karimi et al., 2017).…”