Inducing local DNA damage by visible light to study chromatin repair

Solarczyk, Kamil; Zarębski, Mirosław; Dobrucki, Jurek

doi:10.1016/j.dnarep.2012.09.008

Cited by 24 publications

(25 citation statements)

References 23 publications

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“…The combination of this sensitizer with irradiation at 405 nm seems to trigger an unusual and yet poorly characterized response (Dinant et al, 2007). In general, photosensitizers may have undesired effects on chromatin structure and cellular metabolism, not to mention the fact that they will mediate additional damage throughout the imaging procedure performed with visible light (Solarczyk et al, 2012). …”

Section: Laser Microirradiation Methods For the Induction Of Localizementioning

confidence: 99%

“…This technique requires specialized optics with high UV transmittance. In addition, Solarczyk et al demonstrated selective induction of DNA strand breaks at 488 nm at power levels normally used in confocal imaging (Solarczyk et al, 2012). Independently of the wavelength used, linear absorption occurs throughout the entire irradiation path.…”

Section: Laser Microirradiation Methods For the Induction Of Localizementioning

confidence: 99%

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Highlighting the DNA damage response with ultrashort laser pulses in the near infrared and kinetic modeling

Ferrando‐May¹,

Tomas²,

Blumhardt³

et al. 2013

Front. Genet.

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Our understanding of the mechanisms governing the response to DNA damage in higher eucaryotes crucially depends on our ability to dissect the temporal and spatial organization of the cellular machinery responsible for maintaining genomic integrity. To achieve this goal, we need experimental tools to inflict DNA lesions with high spatial precision at pre-defined locations, and to visualize the ensuing reactions with adequate temporal resolution. Near-infrared femtosecond laser pulses focused through high-aperture objective lenses of advanced scanning microscopes offer the advantage of inducing DNA damage in a 3D-confined volume of subnuclear dimensions. This high spatial resolution results from the highly non-linear nature of the excitation process. Here we review recent progress based on the increasing availability of widely tunable and user-friendly technology of ultrafast lasers in the near infrared. We present a critical evaluation of this approach for DNA microdamage as compared to the currently prevalent use of UV or VIS laser irradiation, the latter in combination with photosensitizers. Current and future applications in the field of DNA repair and DNA-damage dependent chromatin dynamics are outlined. Finally, we discuss the requirement for proper simulation and quantitative modeling. We focus in particular on approaches to measure the effect of DNA damage on the mobility of nuclear proteins and consider the pros and cons of frequently used analysis models for FRAP and photoactivation and their applicability to non-linear photoperturbation experiments.

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Section: Laser Microirradiation Methods For the Induction Of Localizementioning

confidence: 99%

Section: Laser Microirradiation Methods For the Induction Of Localizementioning

confidence: 99%

Highlighting the DNA damage response with ultrashort laser pulses in the near infrared and kinetic modeling

Ferrando‐May¹,

Tomas²,

Blumhardt³

et al. 2013

Front. Genet.

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show abstract

“…A recent report by Solarczyk et al utilized 488 nm visible light to induce DNA damage (46). With an applied laser energy of 17 mJ, recruitment of XRCC1 and LIGIII were seen at sites of induced DNA damage, as were phosphorylated ataxia telangiectasia-mutated gene (ATM), γH2AX, and replication protein A (RPA), indicating that 488 nm light created a mixture of SSBs and DSBs.…”

Section: Dna Damage Induced With Visible Wavelengthsmentioning

confidence: 99%

Micro-irradiation tools to visualize base excision repair and single-strand break repair

Gassman

Wilson

2015

DNA Repair

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Microscopy and micro-irradiation imaging techniques have significantly advanced our knowledge of DNA damage tolerance and the assembly of DNA repair proteins at the sites of damage. While these tools have been extensively applied to the study of nucleotide excision repair and double-strand break repair, their application to the repair of oxidatively-induced base lesions and single-strand breaks is just beginning to yield new insights. This review will focus on examining micro-irradiation techniques reported to create base lesions and single-strand breaks; these lesions are considered to be primarily addressed by proteins involved in the base excision repair (BER) pathway. By examining conditions for generating these DNA lesions and reviewing information on the assembly and dissociation of repair complexes at the induced lesion sites, we hope to promote further investigations into BER and to stimulate further development and enhancement of these techniques for the study of BER.

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“…The wavelength used to image ethidium bromide-stained halos (546 nm excitation) induces DNA damage 15–18 , by introducing both double and single strand breaks. Cells that start with a bright RN and have more DNA associated to the nuclear matrix (class Ia) appear to withstand more stochastic damage before DNA is released and can diffuse away.…”

Section: Resultsmentioning

confidence: 99%

Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos

Wilson

Coverley

2017

Sci Rep

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Inducing local DNA damage by visible light to study chromatin repair

Cited by 24 publications

References 23 publications

Highlighting the DNA damage response with ultrashort laser pulses in the near infrared and kinetic modeling

Highlighting the DNA damage response with ultrashort laser pulses in the near infrared and kinetic modeling

Micro-irradiation tools to visualize base excision repair and single-strand break repair

Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos

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