Inducing high nitrite accumulation via modulating nitrate reduction power and carbon flux with Thauera spp. selection

Fan, Jiarui; Du, Rui; Li, Cong; Liu, Qingtao; Peng, Yongzhen

doi:10.1016/j.biortech.2022.127188

Cited by 35 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7), including norank_f_A4b (30.99%), norank_f_LD‐RB‐34 (4.93%), norank_f_Saprospiraceae (4.67%), Candidatus_Competibacter (3.57%), Ahniella (3.17%) and Terrimonas (3.00%). Despite many previous studies having shown that Thauera is a dominant PD bacteria, the relative proportion of this genus in the present study was just 1.24% 44‐46 . Terrimonas (3.00%), Dokdonella (0.11%) and norank_f_Rhodanobacteraceae (0.07%) are commonly identified in PD systems 47,48 .…”

Section: Resultscontrasting

confidence: 94%

“…Despite many previous studies having shown that Thauera is a dominant PD bacteria, the relative proportion of this genus in the present study was just 1.24%. [44][45][46] Terrimonas (3.00%), Dokdonella (0.11%) and norank_f_Rhodanobacteraceae (0.07%) are commonly identified in PD systems. 47,48 As NAR could exceed 90% in the optimized system, there are likely to be other unknown bacterial genera that play an important role in PD.…”

Section: Pd Microbial Community Structure Analysismentioning

confidence: 99%

See 1 more Smart Citation

Partial denitrification in domestic sewage and nitrate wastewater: study of the initiation, influencing factors and operational performance

Ma,

Qin,

Chen

et al. 2023

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BackgroundPractical application of the anaerobic ammonium oxidation (anammox) process has been limited by the difficulty of obtaining a stable supply of NO2‐‐N. In this study, partial denitrification was utilized as a novel approach to obtain nitrite for anammox, investigating the initiation, influencing factors and performance of partial denitrification in the simultaneous treatment of domestic sewage and nitrate wastewater.ResultsWhen the influent concentration of NO3‐‐N was 40 mg/L and the chemical oxygen demand to nitrate (COD/NO3‐‐N) ratio was 2.7, the NO2‐‐N accumulation rate exceeded 80%, with an effluent NO2‐‐N concentration of 35.64 mg/L. Furthermore, the partial denitrification performance was good. When the volume ratio of domestic sewage and nitrate wastewater was adjusted to 2:3 and sodium acetate was added to form a COD/NO3‐‐N ratio of 3.6, the effluent NO2‐‐N/NH4+‐N ratio was 1.24 and the effluent NO2‐‐N concentration and average NO2‐‐N accumulation rate reached >31.24 mg/L and 83.38%, respectively, effectively ensuring influent requirements for anammox.ConclusionThe NO3‐‐N reduction rate , NO3‐‐N removal rate and NO2‐‐N accumulation rate were positively correlated with the influent nitrate concentration when ranging between 15‐40 mg/L. An appropriate increase in the influent COD/NO3‐‐N ratio was conducive to a reduction in the time required for maximum levels of NO2‐‐N accumulation to occur, with an influent NO3‐‐N concentration of 40 mg/L. The partial denitrification system could simultaneously treat domestic sewage and nitrate wastewater, effectively ensuring that the generated effluent met the influent requirements for subsequent anammox.This article is protected by copyright. All rights reserved.

show abstract

Section: Resultscontrasting

confidence: 94%

Section: Pd Microbial Community Structure Analysismentioning

confidence: 99%

Partial denitrification in domestic sewage and nitrate wastewater: study of the initiation, influencing factors and operational performance

Ma,

Qin,

Chen

et al. 2023

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…The most dominant genus in each fraction was identified as Thauera , and its abundance increased noticeably as the size decreased. The Thauera bacteria were widely reported to be a major contributor to high NO 2 – –N accumulation in the PD process. , In this study, Thauera accounted for up to 59.4% of flocs but only 24.6% in the largest granules. This was well coincided with the PD activity in different-sized sludge.…”

Section: Resultsmentioning

confidence: 68%

Spatiotemporal Assembly and Immigration of Heterotrophic and Anammox Bacteria Allow a Robust Synergy for High-Rate Nitrogen Removal

Du,

Liu,

et al. 2023

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

The novel partial denitrification-driven anammox (PD/A) is an energy-efficient method for nitrogen removal from wastewater. However, its stability and efficiency are impeded by the competition between heterotrophic denitrifying bacteria and relatively slow-growing anammox bacteria. In this study, a PD/A granular sludge system was developed, which achieved a nitrogen removal efficiency of 94% with 98% anammox contribution, even as the temperature dropped to 9.6 °C. Analysis of bacterial activity in aggregates of different sizes revealed that the largest granules (>2.0 mm) exhibited the highest anammox activity, 2.8 times that of flocs (<0.2 mm), while the flocs showed significantly higher nitrite production rates of PD, more than six times that of the largest granules. Interestingly, fluorescent in situ hybridization (FISH) combined with confocal laser scanning microscopy (CLSM) revealed a nest-shaped structure of PD/A granules. The Thauera genus, a key contributor to PD, was highly enriched at the outer edge, providing substrate nitrite for anammox bacteria inside the granules. As temperature decreased, the flocs transformed into small granules to efficiently retain anammox bacteria. This study provides multidimensional insights into the spatiotemporal assembly and immigration of heterotrophic and autotrophic bacteria for stable and high-rate nitrogen removal.

show abstract

“…At the genus level, Thauera belonging to Proteobacteria phylum dominated in all PD granules (Figure 6b), which were mainly responsible for the nitrite production being maintained. 46,47 Further, it was found that the relative proportion of Thauera was not reduced but slightly increased from 65.5 to 71.0% when the influent nitrate was elevated from 720 to 960 mg N/L (Table S3), which, on the other hand, suggests that the PD granule disintegration did not influence the performance of nitrite production. The norank_p__Gracilibacteria was the second genus, similar to Gracilibacteria illustrated above, likely to be related to maintaining the granule structure.…”

Section: Microbial Community Shift At Increasinglymentioning

confidence: 94%

Disintegration of Partial Denitrification Granules at High Nitrate Concentration

et al. 2022

Self Cite

View full text Add to dashboard Cite

It is well acknowledged that high nitrate concentration would negatively affect the biological denitrification activity, especially in the presence of nitrite accumulation. In this study, a novel granular partial denitrification (PD) process was developed, and the impact of nitrate concentration (120−960 mg N/L) on PD granules was investigated by close monitoring of the operational performance, physicochemical characteristics, and microbial community. Results indicated that the effluent nitrite increased with the nitrate concentration, with a maximum value of 492.4 mg N/L obtained. And the maximum denitrification activity in situ was found not to be decreased despite high nitrite accumulation; it was maintained at 0.24 g N/g VSS/h, suggesting the high tolerance of PD granules to the nitrite. The granule size increased with the influent nitrate, and the compact PD granules, however, were observed to be disintegrated at 960 mg N/L of nitrate, which was likely attributed to the substrate diffusion limitation in larger granules, with the cavity formed in its inner part. In addition, microbial analysis revealed that bacterial richness and the diversity of PD granules were decreased, and Gracilibacteria that was supposed to be responsible for maintaining the PD granule structure was largely reduced at a high nitrate concentration, which was likely to be another major reason for the disintegration. This study revealed the potential causes for the disintegration of PD granules, which offer theoretical support and technical guidance to ensure the stability of PD granules in treating high nitrate wastes for efficient nitrite production.

show abstract

Inducing high nitrite accumulation via modulating nitrate reduction power and carbon flux with Thauera spp. selection

Cited by 35 publications

References 38 publications

Partial denitrification in domestic sewage and nitrate wastewater: study of the initiation, influencing factors and operational performance

Partial denitrification in domestic sewage and nitrate wastewater: study of the initiation, influencing factors and operational performance

Spatiotemporal Assembly and Immigration of Heterotrophic and Anammox Bacteria Allow a Robust Synergy for High-Rate Nitrogen Removal

Disintegration of Partial Denitrification Granules at High Nitrate Concentration

Contact Info

Product

Resources

About