Inducible nitric oxide synthase in the placenta of pregnant women with preeclampsia

Abstract

Preeclampsia (PEC) is the cause of fetal and maternal death. Inducible nitric oxide synthase (iNOS) found in macrophages, smooth muscle cells and endothelial cells. The main objective was to detect the fluorescence iNOS in placenta. Cotyledons cuts were made, they were placed in 10% formalin. And on the slides washed with buffer solution, oxygenated water with methanol, using 10% pig serum, anti iNOS Rabbit pAb solution, streptavidin-fluorescein. Microscope Carl Zeiss and Image-Pro 7.0 software was used. Placentas of pregnant women with PEC without pathology were analyzed. Increased uptake of fluorescein in erythrocytes of women without PEC was found, patients who developed PEC was remarkable decreased uptake of fluorescein. Decreasing iNOS activity is evident in preeclampsia.