Inducible Gene Targeting in Mice Using the Cre/loxSystem

Sauer, Brian

doi:10.1006/meth.1998.0593

Cited by 675 publications

(428 citation statements)

References 60 publications

Supporting

Mentioning

415

Contrasting

Unclassified

Order By: Relevance

“…SLICK is based on the popular cre/loxP system that has been used extensively to achieve conditional gene knockout in mice 17,34,35 . The availability of mice harboring conditional knockout alleles is continually increasing and a large scale project to generate "floxed" conditional knockout alleles is underway 1 .…”

Section: Discussionmentioning

confidence: 99%

Single-neuron labeling with inducible Cre-mediated knockout in transgenic mice

et al. 2008

View full text Add to dashboard Cite

To facilitate functional analysis of neuronal connectivity in a mammalian nervous system tightly packed with billions of cells, we developed a new technique that allows inducible genetic manipulations within fluorescently labeled single neurons in mice. We term this technique SLICK for Single-neuron Labeling with Inducible Cre-mediated Knockout. SLICK is achieved by coexpressing a drug-inducible form of cre recombinase and a fluorescent protein within the same small subsets of neurons. Thus, SLICK combines the powerful cre recombinase system for conditional genetic manipulation and the fluorescent labeling of single neurons for imaging. We demonstrate efficient inducible genetic manipulation in several types of neurons using SLICK. Furthermore, we apply SLICK to eliminate synaptic transmission in a small subset of neuromuscular junctions. Our results provide evidence for the long-term stability of inactive neuromuscular synapses in adult animals. More broadly, these studies demonstrate a cre-LoxP compatible system for dissecting gene functions in single identifiable neurons.

show abstract

Section: Discussionmentioning

confidence: 99%

Single-neuron labeling with inducible Cre-mediated knockout in transgenic mice

et al. 2008

View full text Add to dashboard Cite

show abstract

“…In order to test the role of specific genes and gene products in normal intestinal development or in pathological processes, genetic tools are needed that allow the creation of null alleles or expression of exogenous genes in intestinal epithelium, with a high degree of spatiotemporal experimental control. This can be achieved by the elegant Cre/lox recombination system (Chambers, 1994;Sauer, 1998); for further reading we recommend volume 26 of genesis (2000), which is dedicated to the Cre-lox system.…”

mentioning

confidence: 99%

Tissue‐specific and inducible Cre‐mediated recombination in the gut epithelium

Marjou

Janssen

Chang

et al. 2004

Genesis

900

862

View full text Add to dashboard Cite

Summary: We generated two complementary systems for Cre-mediated recombination of target genes in the mouse digestive epithelium and tested them with a Crereporter mouse strain. Cre was expressed under the control of a 9 kb regulatory region of the murine villin gene (vil-Cre). Genetic recombination was initiated at embryonic day (E) 9 in the visceral endoderm, and by E12.5 in the entire intestinal epithelium, but not in other tissues. Cre expression was maintained throughout adulthood. Furthermore, transgenic mice bearing a tamoxifen-dependent Cre recombinase (vil-Cre-ER T2 ) expressed under the control of the villin promoter were created to perform targeted spatiotemporally controlled somatic recombination. After tamoxifen treatment, recombination was detectable throughout the digestive epithelium. The recombined locus persisted for 60 days after tamoxifen administration, despite rapid intestinal cell renewal, indicating that epithelial progenitor cells had been targeted. The villin-Cre and villin-Cre-ER T2 mice provide valuable tools for studies of cell lineage allocation and gene function in the developing and adult intestine. genesis 39: 186 -193, 2004.

show abstract

“…Given that compensation in rodents may be a concern, the cre-lox and creER-lox systems combined with tamoxifen induction protocols can be used to control the regional and temporal expression of genes of interest [65][66][67]. The creER-lox system can be used to systematically dissect whether loss of a protein in a particular brain region or neuronal population at a specific point of time in the life of the animal can result in dystonia.…”

Section: Alternative Approaches For the Generation Of Rodent Models Omentioning

confidence: 99%