Inducible Expression and Phosphorylation of Coactivator BOB.1/OBF.1 in T Cells

Zwilling, Stefan; Dieckmann, Andreas; Pfisterer, Petra; Angel, Peter; Wirth, Thomas

doi:10.1126/science.277.5323.221

Cited by 89 publications

(86 citation statements)

References 22 publications

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“…Oct-2 is expressed predominantly in the B-cell lineage; however, recent studies demonstrated that B cell-restricted gene regulation is dependent on B cell-specific coactivator BOB.1/ OBF/OCA-B (2-5). Expression of Oct-2 and BOB.1/OBF/OCA-B is also induced by antigenic stimulation of T cells (6,7). Thus, the octamer transcription system may play a role in gene expression during T cell activation.…”

mentioning

confidence: 98%

Octamer Transcription Factors Up-regulate the Expression of CCR5, a Coreceptor for HIV-1 Entry

Moriuchi¹,

Moriuchi²

2001

Journal of Biological Chemistry

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T cell activation can induce expression of CCR5, a major coreceptor for macrophage-tropic (R5) human immunodeficiency virus type 1 (HIV-1). Here we report that overexpression of the Oct-2 transcription factor and octamer coactivator BOB.1/OBF/OCA-B, both of which are induced in T cells following T cell receptor signaling, synergistically up-regulates CCR5 promoter activity via interaction with an octamer motif on the promoter. We also show that the octamer transcription factors can increase cell surface expression of CCR5 and fusogenicity of the cells with R5 HIV-1 Env. These results suggest that octamer transcription factors may play a critical role in the induction of CCR5 expression on, and thereby susceptibility to, R5 HIV-1 of T cells following antigenic stimulation.

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mentioning

confidence: 98%

Octamer Transcription Factors Up-regulate the Expression of CCR5, a Coreceptor for HIV-1 Entry

Moriuchi¹,

Moriuchi²

2001

Journal of Biological Chemistry

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“…OBF-1 expression was previously found to be mostly B cell-restricted but could be induced in T cell lines and primary murine thymocytes after in vitro stimulation with phorbol myristic acetate (PMA) and ionomycin (18,19). However, the physiological role of OBF-1 in T cells has remained unclear and in OBF-1 Ϫ/Ϫ mice T cell development and function appear normal (10,20).…”

Section: Resultsmentioning

confidence: 99%

“…OBF-1 expression is largely restricted to B cells (10), but it can also be induced in T lymphocytes by costimulation in vitro (18,19). The phenotype observed in OBF-1-deficient mice clearly coincides with the expression pattern of the OBF-1 gene in B cells, which peaks at two distinct time points in B cell development: at the immature B cell stage in the bone marrow and with the highest expression levels in GCs and GC-derived B cell lymphomas (20,21).…”

mentioning

confidence: 99%

The Ets factor Spi-B is a direct critical target of the coactivator OBF-1

Bartholdy

Roure

Bordon

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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OBF-1 (Bob.1, OCA-B) is a lymphoid-specific transcriptional coactivator that associates with the transcription factors Oct-1 or Oct-2 on the conserved octamer element present in the promoters of several ubiquitous and lymphoid-specific genes. OBF-1-deficient mice have B cell-intrinsic defects, lack germinal centers, and have severely impaired immune responses to T cell-dependent antigens. Crucial genes that are regulated by OBF-1 and that might explain the observed phenotype of OBF-1 deficiency have remained elusive to date. Here we have generated transgenic mice expressing OBF-1 specifically in T cells and examined these together with mice lacking OBF-1 to discover transcriptional targets of this coactivator. Using microarray analysis, we have identified the Ets transcription factor Spi-B as a direct target gene critically regulated by OBF-1 that can help explain the phenotype of OBF-1-deficient mice. Spi-B has been implicated in signaling pathways downstream of the B cell receptor and is essential for germinal center formation and maintenance. The present findings establish a hierarchy between these two factors and provide a molecular link between OBF-1 and B cell receptor signaling.B cell development ͉ gene regulation ͉ transcription factors ͉ transgenic mice

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“…In this cell line, BOB.1/ OBF.1 activity is inducible by treatment with phorbol 12-myristate 13-acetate and ionomycin (23). Phorbol 12-myristate 13-acetate/ionomycin treatment resulted in a large increase in the activities of the Ahd2-like promoter as well as the control promoters (Fig.…”

Section: Bob1/obf1 Target Genes In B Cellsmentioning

confidence: 99%

“…However, expression of functional BOB.1/OBF.1 can be also induced in T lymphocytes by costimulation (23). It is expressed at all stages of B cell development, albeit at various levels.…”

mentioning

confidence: 99%