Inducible Cytochrome P450s in the Fat Body and Malpighian Tubules of the Polyphagous Pests of Spodoptera litura Confer Xenobiotic Tolerance

Abstract

Cytochrome P450 plays vital roles in detoxifying xenobiotics. In this study, SlCYP340A and SlCYP340L expression in the Spodoptera litura fat body and SlCYP332A1, SlCYP6AB12, SlCYP6AB58, SlCYP6AB59, and SlCYP6AN4 expression in the Malpighian tubules were significantly upregulated after cyantraniliprole exposure, and SlCYP6AB58 and SlCYP6AB59 expression levels were simultaneously increased in the Malpighian tubules after gossypol treatment. Drosophila ectopically expressing candidate P450 genes showed that SlCYP332A1, SlCYP6AB12, SlCYP6AB59, SlCYP6AN4, and SlCYP340A conferred cyantraniliprole tolerance. The overexpression of SlCYP6AB58 and SlCYP6AB59 in Drosophila increased the number of eggs laid under the gossypol treatment. Moreover, the knockdown of SlCYP332A1, SlCYP6AB12, SlCYP6AB59, SlCYP6AN4, and SlCYP340A increased S. litura mortality under the cyantraniliprole treatment. Homology modeling and molecular docking results suggested that candidate P450 has the potential to bind with cyantraniliprole. These results indicate that the CYP3 and CYP4 genes participate in cyantraniliprole detoxification and that SlCYP6AB59 may be simultaneously involved in the gossypol tolerance of S. litura.

A Malpighian Tubule-Specific P450 Gene SlCYP9A75a Contributes to Xenobiotic Tolerance in Spodoptera litura

“…Among these CYP3 genes, several P450s identified in our previous study were related to cross-tolerance to cyantraniliprole and gossypol. 42 In addition, we found that a highly abundant CYP9 gene, SlCYP9A75a, shared the highest similarity with its paralogues SlCYP9A75b, clustering into the same branch with other CYP9A genes of S. frugiperda and S. exigua, which were involved in detoxifying insecticides and plant secondary metabolites (Figure 3A). 13 A previous study has demonstrated that SlCYP9A75a and SlCYP9A75b were significantly upregulated after abamectin exposure and has determined that these two genes contributed to abamectin tolerance via transgenic Drosophila bioassays.…”

UDP-Glycosyltransferases UGT350C3 and UGT344L7 Confer Tolerance to Neonicotinoids in Field Populations of Aphis gossypii

“…gossypii, further functional investigations of their roles in neonicotinoid detoxification are needed. The GAL4/UAS system has been widely used to explore the roles of detoxification genes in insect resistance in transgenic Drosophila in vivo. − Overexpressing UGT33J17 and UGT41D10 of S. litura in Drosophila reduced the indoxacarb toxicity to flies .…”

CF2–II Alternative Splicing Isoform Regulates the Expression of Xenobiotic Tolerance-Related Cytochrome P450 CYP6CY22 in Aphis gossypii Glover

“…Further details of the bioassays used are provided in our previous study. 31 The median lethal dose (LD 50 ) and 95% fiducial limits were calculated by probit analysis using SPSS Statistics (IBM SPSS Statistics 26 software, Chicago, IL, USA) and PoloPlus 2.00 software (LeOra Software, USA).…”

“…Topical application (F1 of the UAS- CYP6CY22 and Act5C-GAL4 hybrids) and feeding (F1 of the UAS- CYP6CY22 and Esg-GAL4 hybrids) methods were used to assess the susceptibility of straight-wing-phenotype adult female flies to cyantraniliprole. Further details of the bioassays used are provided in our previous study . The median lethal dose (LD 50 ) and 95% fiducial limits were calculated by probit analysis using SPSS Statistics (IBM SPSS Statistics 26 software, Chicago, IL, USA) and PoloPlus 2.00 software (LeOra Software, USA).…”