Inducible Antimicrobial Compounds (Halal) Production in Honey Bee Larvae (Apis mellifera) from Rumaida, Taif by injecting of various dead Microorganisms extracts

Abstract

In the current study, the antimicrobial compounds in induced Honey bee larvae (Apis mellifera) from the Taif region were extracted from hemolymph and assigned as Halal safe product. Their minimum inhibitory concentrations (MIC) were evaluated. Five different microbes, namely Escherichia coli, Bacillus subtilis, Pseudomonas aureus, Candida albicans and Micrococous luteus were used in this study by the agar diffusion assay method. The antibacterial activity of induced honey bee larvae was studied against the 5 above mentioned organisms and the MIC was differentiated from their inhibition for varies microbial growth. The inducible antimicrobial compounds extracted from honey bee larvae hemolymph, after one day at room temperature were used for minimum inhibitory concentration studied. The results showed appreciable antimicrobial activity (6±1) when the larvae were injected with 15µl Micrococus leutus total protein extract and when injected with the same concentration (i.e. 15µl Pseudomonas aureus), they showed low activity (4±1) against Candida albicans. The Amoxycillin was used as a standard positive control. The negative control of the Phosphate buffer solvent (PBS) and larvae did not show any activity against all five organisms. All dilutions were executed, minimum inhibitory concentration studies ranging from 10 -1 to 10 -4 did not show any activity. The most inhibition was obtained with induced young larvae at various ages (L4 young larvae and L5-youngest larvae) against Candida albicans.