Inducibility of human β-interferon gene in mouse L-cell clones

Abstract

Transfer of a 36-kilobase piece of human DNA containing the beta-interferon (IFN-beta) gene into mouse Ltk-cells leads to transient expression of human interferon even without an exogenous inducer. A low level of human interferon expression is also found in most stable clones containing the transferred DNA. With double-stranded RNA or Newcastle disease virus (NDV) as inducer, human interferon expression is greatly increased. The induced transcript is identical to normal human IFN-beta mRNA. Neighbouring genes contained on the transferred DNA are co-induced but are not essential for the production of human interferon in mouse L cells.

Sequences involved in the regulated expression of the human interferon-beta1 gene in recombinant SV40 DNA vectors replicating in monkey cells.

“…Even though the gene was expressed from an external promoter, RNA with authentic 5' ends was almost absent unless the CV-1 cells were induced. Poly(rI)(rC)-dependent induction is, thus, characterized by the accumulation of RNA which starts at the IFN-31 genes cap site, as found also in stable co-transformants (Hauser et al, 1982;Canaani and Berg, 1982). This accumulation may result from an effect of induction on RNA polymerase initiation at this site, or else from an effect on processing or stabilization of RNA with correct 5' ends.…”

“…These genes lack introns and can be expressed in bacteria (Nagata et al, 1980;Mory et al, 1981). When transferred into heterologous cells by the gene co-transformation procedure (Wigleret al, 1979), several human IFN genes were shown to retain their inducibility by IFN inducers in the stable mouse cell clones isolated (Ohno and Taniguchi, 1982;Mantei and Weissmann, 1982;Reyes et al, 1982;Hauser et al, 1982;Canaani and Berg, 1982). The use of stable co-transformants suffers, however, from the drawback that the time required to select transformants may allow DNA rearrangements and unknown interactions with host *To whom reprint requests should be sent.…”

The gene S promoter of hepatitis B virus confers constitutive gene expression

“…In previous reports concerning HIFN-synthesis in transfected animal cells, HIFN-a gene expression was inducible (34)(35)(36)(37)(38)). The results presented here show that constitutive synthesis is also possible at a level comparable to that obtained after poly (rI:rC) induction.…”

Difference in the Production of Human Interferon- and -beta in Mouse Cells

“…In cells transfected with the HulFN-fl gene, both IFN-specific mRNA and IFN were synthesized (Canaani & Berg, 1982;Hauser et al, 1982;Maroteaux et al, 1983;Tavernier et al, 1983); however, in cells transfected with the HulFN-0q gene only IFN-specific mRNA, but not the IFN itself, could be detected upon induction by Sendai virus (Mantei & Weissmann, 1982). The low sensitivity of the IFN detection system might explain this observation, since the relatively insensitive human WISH cells were employed for assaying HuIFN-oq (Weck et al, 1981).…”