Inducibility of chromosome aberrations by steroid hormones in cultured Chinese hamster ovary cells

Effects of estrogenic chemicals on development.

“…Although to date no causal relationship has been proved to link steroid-hormone effects to the induction of chromosomal alterations in an animal system, steroid hormones have been shown to be capable of producing chromosomal abnormalities in cultured cells (1,2). In addition, Banduhn and Obe (3) showed that both diethylstilbestrol (DES) and estradiol are capable of inducing genomic mutations in cultured cells, and Wheeler et al (4) showed that estradiol and DES are potent inhibitors of mitosis in in vitro cell cultures.…”

Involvement of genotoxic effects in the initiation of estrogen-induced cellular transformation: Studies using Syrian hamster embryo cells treated with 17?-estradiol and eight of its metabolites

“…However, somatic mutations at the Na ϩ /K ϩ ATPase and hprt loci were not elicited in SHE cells by E 1 (Table II). Kochhar (1985) found no clastogenic activity of E 1 by using Chinese hamster ovary (CHO) cells. No mutagenic activity of E 1 was also noted by Drevon et al (1981) in Chinese hamster V79 cells with a cell-mediated system using primary hepatocytes from a rat.…”

“…There are few other reports about the genotoxic effects of E 3 . E 3 induces chromosome aberrations in CHO cells (Kochhar, 1985) but does not induce aneuploidy in cultured human lymphocytes (Hill and Wolff, 1983).…”