Induced thermal tolerance and heat shock protein synthesis in Chinese hamster ovary cells

Li, Gloria C.; Petersen, Nancy S.; Mitchell, Herschel K.

doi:10.1016/0360-3016(82)90386-8

Cited by 70 publications

(20 citation statements)

References 13 publications

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“…The relationship of the heat shock proteins and thermotolerance has been investigated by several workers. It has been established that a correlation exists between the induction of the heat shock proteins and the acquisition of thermotolerance in exponentially growing cells (8,10,13). This correlation also exists for stationary-phase cells, in which high levels of synthesis of a subset of the heat shock proteins are maintained when overall protein synthesis declines dramatically (1).…”

Section: Resultsmentioning

confidence: 99%

Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae

Plesset¹,

Ludwig²,

Cox³

et al. 1987

J Bacteriol

106

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We showed that the heat killing ,curve for exponentially growing Saccharomyces cerevisiae was biphasic. This suggests two populations of cells with different therpial killing characteristics. Whep exponentially growing cells separated into cell cycle-specific fractions via centrifugal elutriation were, heat shocked, the fractions enriched in smnall unbudded cells showed greater resistance to heat killing than did other cell cycle fractions. The lethal effects of extreme temperature on Saccharomyces cerevisiae have been described. Exponentially growing cells are much more sensitive than are stationary-phase cells to a thermal shock (15). Thus, resting or quiescent cells are more thermotolerant than growing cells are. This heat resistance is acquired as the cells pass from the exponentially growing to the stationary state (12). Schenberg-Frascino and Moustacchi (15) isolated small unbudded cells from stationary cultures and observed their thermotolerance as they progressed through the first cell cycle into exponential growth. They observed that thermotolerance is decreased in budding cells. Yeast cells, when starved for an essential nutrient such as nitrogen, phosphorous, or sulfur, cease dividing and arrest their cell division as unbudded cells. The thermotolerance of cells starved for any of these nutrients resembles that of stationary-phase cells, and it has been suggested that this thermotolerance is a general characteristic of resting cells (11).Our aim was to clarify the relationship between the cell cycle and thermotolerance. To this end we examined the effect of the cell cycle position on the thermotolerance of S. cerevisiae in exponentially growing cultures by using the elutriator rotor to separate cells by size and cell cycle position (9). We also examined the effects on the cell cycle of a mild heat shock, during which the cells acquire thermotolerance (10, 13). In addition, we used several methods to arrest cells in the unbudded state, and we determined the thermotolerance of the resulting populations. The methods included arrest via deprivation of essential nutrients, alphafactor arrest, and the use of temperature-sensitive cell division cycle mutants. Our results demonstrate that a distinct population of unbudded cells was more thermotolerant than were cells in other morphological stages in the cell cycle. We suggest that this thermotolerance is associated with a defined state, G0, which has synthetic and physiological properties distinct from those of other unbudded cells. MATERIALS AND METHODSStrains. The following strains were used: SKQ2n (a/a adell+ +/ade2 +Ihisl); A364A (a lys2 tyrl his7 gall adel ade2 ural); cdc7-1 (ts124); cdc25-1 (ts321); cdc33-1 (El7a), derived from A364A (4; provided by L. Hartwell); cdc35-1 (BR214-4a; a adel ural his7 arg4 trpl); and cdc36-12 (ST21; a met2 cyh2) (provided by L. Hartwell).Media and cell growth.

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Section: Resultsmentioning

confidence: 99%

Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae

Plesset¹,

Ludwig²,

Cox³

et al. 1987

J Bacteriol

106

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“…The induction of thermal tolerance has been found to be associated with HSP up regulation. (40)(41) Due to concerns about thermal tolerance, hyperthermia is typically delivered no more than a few times per week, generally with at least 72 hours between treatments.…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Hyperthermia, Radiation and Chemotherapy: The Role of Heat in Multidisciplinary Cancer Care

Hurwitz

Stauffer

2014

Seminars in Oncology

118

104

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“…A correlation between the development of thermotolerance and the synthesis of HSPs has been reported, although the mechanisms underlying the thermotolerance remain unknown (19,21). The loss of HSPs and the decay of thermotolerance have also been shown to be related (18).…”

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confidence: 99%

Accumulation of Heat Shock Proteins in Field-Grown Cotton

Burke¹,

Hatfield²,

Klein³

et al. 1985

Plant Physiol.

122

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are beat shock proteins and that these proteins can accumulate to substantial levels in field-stressed plants.Plants growing in environments with ample water supplies maintain, through transpiration, leaf temperatures at or below air temperatures. Plants exposed to drought conditions experience declining soil water levels which ultimately result in stomatal closure and reduced transpiration. Drought-induced stomatal closure limits carbon assimilation, yet may optimize the water use efficiency of the plant on a daily basis (9). As a consequence of the reduction in transpiration, leaf temperatures increase above the temperature of the surrounding air (12). The elevated leaf temperatures may limit dry matter accumulation because of increased respiration, reduced photosynthesis, and cellular damage.Living organisms have developed several endogenous protection systems which provide thermal tolerance (I 1, 13). One of these protection systems involves an acquired heat resistance mechanism associated with the synthesis and accumulation of specific proteins (HSPs2 [1,15]). These proteins have been identified in numerous animal (20,22,33) and plant (7,14,16,27,29) species by following the incorporation oflabeled amino acids into proteins during exposure to elevated temperatures (>37°C). Because they have only been detected by autoradiography and not by Coomassie blue or silver staining techniques, it has been suggested that heat shock proteins do not accumulate to substan-' Present address:

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Induced thermal tolerance and heat shock protein synthesis in Chinese hamster ovary cells

Cited by 70 publications

References 13 publications

Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae

Effect of cell cycle position on thermotolerance in Saccharomyces cerevisiae

Hyperthermia, Radiation and Chemotherapy: The Role of Heat in Multidisciplinary Cancer Care

Accumulation of Heat Shock Proteins in Field-Grown Cotton

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