Induced systemic resistance in wounded rice plants

Salicylic acid-independent plant defence pathways

“…Wound-induced systemic resistance against fungal pathogens has recently been demonstrated as well. Wounding of young rice plants induces a systemic resistance response that leads to protection against infection by the rice blast fungus Magnaporthe grisea in the absence of PR gene expression 30 . Jasmonic acid has emerged as an important signal in the wound response 31 .…”

Copper Amine Oxidase Expression in Defense Responses to Wounding and Ascochyta rabiei Invasion

“…Wounding different plant organs or interaction with pathogens induce local and systemic accumulation of defenserelated proteins (Hammond-Kosack and Jones, 1996;Ryals et al, 1996;Ryan, 2000). The study of signaling events inducing local and systemic responses led to the discovery of systemin, jasmonates, ethylene, salicylic acid (SA), and abscisic acid (ABA) as signal molecules (Peñ a-Cortés et al, 1989; Farmer and Ryan, 1990; Pearce et al, 1991;Xu et al, 1994; O'Donnell et al., 1996;Schweizer et al, 1998;van Loon et al, 1998; Knoester et al, 1999).The existence of multiple defense strategies and complex signaling networks leads to an enhanced defense capacity of the plants. The signal transduction pathways of wounding and pathogen attack may be common, different, or exclusive, depending on the biological system, but likewise the establishment of defense mechanisms requires the presence or accumulation of hydrogen peroxide (H 2 O 2 ; Sutherland, 1991; Mehdy, 1994; Hammond-Kosack et al, 1996).…”

“…Wounding different plant organs or interaction with pathogens induce local and systemic accumulation of defenserelated proteins (Hammond-Kosack and Jones, 1996;Ryals et al, 1996;Ryan, 2000). The study of signaling events inducing local and systemic responses led to the discovery of systemin, jasmonates, ethylene, salicylic acid (SA), and abscisic acid (ABA) as signal molecules (Peñ a-Cortés et al, 1989; Farmer and Ryan, 1990; Pearce et al, 1991;Xu et al, 1994; O'Donnell et al., 1996;Schweizer et al, 1998;van Loon et al, 1998; Knoester et al, 1999).…”

Enhanced Resistance to Blast Fungus and Bacterial Blight in Transgenic Rice Constitutively ExpressingOsSBP, a Rice Homologue of Mammalian Selenium-binding Proteins

“…To analyze the phytoalexin accumulation and expression of the PR gene, T 1 seedlings (30 days after sowing) were sprayed with a conidial suspension (2 Â 10 6 conidia/ml containing a 0.05% Tween 20 solution) of a virulent race (007) or an avirulent race (031, MAFF305494) of the fungus. After the inoculation, the seedlings were kept in a moist chamber at 25 C for 36 h in the dark, before being transferred to a greenhouse at [25][26][27][28][29][30] C. For inoculation with the bacterial blight, the T7174R bacterium, a spontaneous rifampicin-resistant mutant from virulent strain T7174 (race 1), was suspended in water at 10 8 cells/ml. The leaves were then inoculated with the bacterium by the scissors-dip method.…”

“…To test whether the expression of PR genes was different in the OsSBP transformants, we monitored the temporal expression of the two PR genes, PBZ1 and PR1, 2,11,28,29) by northern blotting after infection. RNA was isolated from the rice leaves that had been infected with either the avirulent or virulent strain of rice blast.…”