Induced Stereo and Substrate Selectivity of Bio-Imprinted α-Chymotrypsin in Anhydrous Organic Media

Biocatalysis in non-conventional media: Medium engineering aspects (Technical Report)

“…In a microaqueous reaction mixture the a-chymotrypsin prepared in this way exhibits high selectivity in the synthesis of the D-form of the ethyl ester of the N-acetylated amino ester present during precipitation. When precipitation of a-chymotrypsin is done in the presence of the L-form of the N-acetylated amino acid or in the absence of the Disomer, no esterification of the N-acetylated amino acid occurs (Stihl et al, 1991, Mansson et al 1992.…”

Horse Liver Alcohol Dehydrogenase can Accept NADP+ as Coenzyme in High Concentrations of Acetonitrile

“…In one case, chymotrypsin was precipitated by addition of 1-propanol in the presence of N-acetyl-D-tryptophan (%&I et al, 1990Minsson et al, 1992). After careful drying, the enzyme precipitate was able to catalyze, not only the synthesis of the ethyl ester of N-acetyl-L-tryptophan, but also the synthesis of the corresponding D-ester which was not possible with enzyme precipitated in the presence of the corresponding L-derivative of tryptophan or precipitated without ligand present.…”