Induced Spawning and Egg Quality Evaluation of Red Snapper, <i>Lutjanus campechanus</i>

Bourque, Bradford; Phelps, Ronald P.

doi:10.1111/j.1749-7345.2007.00090.x

Cited by 17 publications

(16 citation statements)

References 31 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The size of the oil globule (0.115 ± 0.013 mm, n = 100) was similar to that reported for other species: for L. argentimaculatus, 0.140 ± 0.005 mm (Emata et al 1994) and 0.14 ± 0.16 mm (Leu et al 2003); for L. griseus, 0.139 mm (Cabrera et al (1998); and for L. campechanus, 0.117 ± 0.015 mm (Bourque and Phelps 2007).…”

Section: Discussionsupporting

confidence: 85%

“…Maturation was detected by inserting a polyethylene tube (0.75 mm inner diameter) into the genital pore, followed by gentle aspiration of ovary tissue (oocytes). In males, a gentle massage of the abdomen followed by sperm release was sufficient indication of mature specimens (Singhagraiwan and Doi 1993, Leu et al 2003, Bourque and Phelps 2007. The fish were transported from the cages to the Marine Biology Station (around 30 min by speed boat), where a gonadal biopsy was taken and analyzed in detail.…”

Section: Spawning Protocolmentioning

confidence: 99%

“…The following fertilization rates have been reported for lutjanid species: for L. argentimaculatus, 0-86.4% by hormonal induction (HI) (Singhagraiwan and Doi 1993), 77.3-80% by HI (Emata 2003), and 74.3% by natural spawning (NS) (Leu et al 2003); for L. analis, 75.7% by HI (Watanabe et al 1998); for L. griseus, 85.9% by HI (Cabrera et al 1998); and for L. campechanus, 1.74-79.0% by HI (Bourque and Phelps 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Induced spawning and larval rearing of spotted rose snapper, Lutjanus guttatus, at the Marine Biology Station, Puntarenas, Costa Rica

Boza-Abarca¹,

Calvo-Vargas²,

Solis-Ortiz³

et al. 2008

Cienc. Mar.

View full text Add to dashboard Cite

Wild spotted rose snapper, Lutjanus guttatus (n = 400, 60–100 g), were captured and grown out in cages during one year (February 2001 to February 2002). When the mature stage was detected by cannula samples, females (n = 9) were induced to spawn with a total dose of 1600 IU of human chorionic gonadotropin (HCG) per kilogram of body weight, given in two injections (56% and 44% of the total dose). Males received only one injection of 200 IU HCG kg–1 body weight, at the time of the second female injection. The sex ratio (female:male) was 1:2. Mean female body weight was 604.9 ± 98.8 g (36.4 ± 2.1 cm). Egg release occurred 9–12 hours after the second injection (latent period), at a temperature of 26.3–28.2ºC, salinity of 30–32‰, and oxygen concentration of 5.5–6.6 g L–1. The fertilization rate was 90% of buoyant eggs, with a mean egg diameter of 0.857 ± 0.044 mm. Hatching occurred 15 hours after spawning (h.a.s.) at 26.3–28.2ºC. Larvae started feeding 65 h.a.s. and the postflexion stage was observed 27 days after spawning (d.a.s.). The critical period was observed 3–5 d.a.s., as in other lutjanid species. At 26 d.a.s., the growth rate was 0.243 mm d–1 and the survival rate was 1.5%. The growth rate of the fingerlings (171 d.a.s.) was 0.2 g d–1, with a mean body weight of 33.36 ± 9.13 g. Naturally spawned larvae grew until 30 d.a.s., when massive mortality occurred.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Spawning Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induced spawning and larval rearing of spotted rose snapper, Lutjanus guttatus, at the Marine Biology Station, Puntarenas, Costa Rica

Boza-Abarca¹,

Calvo-Vargas²,

Solis-Ortiz³

et al. 2008

Cienc. Mar.

View full text Add to dashboard Cite

show abstract

“…Latency, mean egg, and oil droplet diameters, as well as incubation period and newly hatched larval size in the present study were similar to those of wild spotted rose snapper spawners from the same area (Ibarra-Castro and Duncan 2007), captive spawners of the same species in Costa Rica (Boza-Abarca et al 2008;Herrera-Ulloa et al 2010), and other snapper species (Lim et al 1985;Leu et al 2003;Bourque and Phelps 2007). The quality of the spawned eggs was usually high in the first as well as in all consecutive spawns.…”

Section: Gnrha-induced Spawning Experimentssupporting

confidence: 82%

GnRHa‐induced Multiple Spawns and Volition Spawning of Captive Spotted Rose Snapper, Lutjanus guttatus, at Mazatlan, Mexico

Ibarra‐Castro

Álvarez‐Lajonchère

2011

J World Aquaculture Soc

View full text Add to dashboard Cite

Sexual maturation and induced spawning treatments were carried out with captive spotted rose snapper, Lutjanus guttatus. A total of 3013 × 10 6 eggs (64.7% were floating) were produced from eight treated females in 42 spawns induced with GnRHa implants during the course of the present study. GnRHa ethylene-vinyl acetate copolymer effective doses were 204 ± 11 μg/kg in June 2005, and 224 ± 13 μg/kg in July 2005. General fertilization was 50.9 ± 34.5% and 12-14 h after spawning, viability of floating eggs was 90.4 ± 12.4%. Mean incubation period at 29-31 C was 18-20 h, and mean hatching was 94.4 ± 8.2% (73-100%). Newly hatched larvae were 2.18 ± 0.15 mm in total length (TL). One month after the last hormone experiment, previously GnRHa-treated and untreated fish began spawning voluntarily. Hormone-treated breeders had higher fecundity than untreated fish, producing 72.5 million eggs versus 13.9 million eggs for the untreated fish, over the following 11 mo. Combined data of volitional spawning for total egg fertilization, viability, hatching, and larval TL were 77.7 ± 1.8%, 90.3 ± 1.3%, 87.9 ± 2%, and 2.50 ± 0.12 mm, respectively. These results can ensure the sustainability of a commercial hatchery.

show abstract

“…Larvae are typically transferred 24 h post hatch to larval tanks where rearing density is lowered to 0.1 larva mL −1 or less. Incubation of red snapper eggs at a density of 2 eggs mL −1 or higher was shown detrimental to larval survival estimated 36 h post hatch (Bourque and Phelps, 2007), but the actual changes of water quality parameters such as levels of dissolved oxygen and un-ionized ammonia induced by elevated density were not reported in this experiment. In addition, this study did not document the kinetics of mortality during development, which prevented evaluating the viability of larvae surviving the early phases of exposures and determining the cumulative effects of prolonged exposures.…”

Section: Introductionmentioning

confidence: 62%

Effects of hypoxia and elevated ammonia concentration on the viability of red snapper embryos and early larvae

Bardon‐Albaret

Saillant

2016

Aquaculture

View full text Add to dashboard Cite

a b s t r a c tThe effects of hypoxic conditions and elevated ammonia concentrations on the viability of embryos and newly hatched larvae of the red snapper (Lutjanus campechanus) were investigated. In all experiments, tested levels of hypoxia or ammonia concentrations were applied to embryos and unfed newly hatched larvae from three different spawns. Exposures began at 1 h post fertilization (pf) and lasted until all individuals in a group had expired. Survival rates were monitored daily in duplicates for each spawn in each treatment. Fertilized eggs exposed to 2 mg L −1 dissolved oxygen (29% saturation) showed complete mortality before hatch while 81% of embryos in control groups (N 85% saturation) hatched and subsequently maintained high survival until 5 days pf. Exposure to a moderate hypoxia (target 3 mg L −1 , 43% saturation) reduced significantly the hatch rate and subsequent survival rates; the magnitude of the difference in survival rate between control and exposed groups increased from 10% at hatch to 45% at 5 days pf. When oxygen concentration was maintained high (83% saturation) until 36 h pf and then progressively reduced to reach 3 mg L −1 at 2 days pf, the survival of exposed embryos and larvae did not differ significantly from those recorded in control groups, although potential delayed or cumulative effects of the treatment after 4 days pf could not be evaluated in this experiment. Embryos exposed to 10 mg L −1 total ammonia (TA-N), which corresponded to unionized ammonia (UIA-N) concentrations ranging between 0.307 and 0.468 mg L −1 in the conditions of the experiment, exhibited significantly reduced hatch rates and complete mortality between 3 and 4 days pf; the latter period corresponds to the onset of exogenous feeding of red snapper. In contrast, control groups (TA-N b 0.26 mg L −1 , UIA-N b 0.006 mg L −1 ) maintained high survival rates beyond 5 days pf indicating potential to successfully initiate exogenous feeding. Exposure to 1 mg L −1 TA-N (0.020 mg L −1 b UIA-N b 0.054 mg L −1 ) did not alter significantly survival with respect to control groups. Significant interactions between the spawn and the tolerance to hypoxia or elevated ammonia were detected in both experiments, indicating that variations among spawns need to be accounted for when determining safe levels for hatchery production. Statement of relevanceAchieving a reliable supply of high quality eggs and larvae is one of the main challenges of the developing marine aquaculture industry. Most studies to date have focused on maternal determinants of egg quality but the viability of embryos and newly hatched larvae can be impacted after fertilization if environmental conditions become unfavorable due to intensive hatchery conditions; this topic is poorly documented in marine fishes to date. This study provides data on the effects of two major stressors acting under high density culture (hypoxia and elevated ammonia concentration) on embryos and newly hatched larvae of the red snapper; the results highlight the importance to conside...

show abstract

Induced Spawning and Egg Quality Evaluation of Red Snapper, Lutjanus campechanus

Cited by 17 publications

References 31 publications

Induced spawning and larval rearing of spotted rose snapper, Lutjanus guttatus, at the Marine Biology Station, Puntarenas, Costa Rica

Induced spawning and larval rearing of spotted rose snapper, Lutjanus guttatus, at the Marine Biology Station, Puntarenas, Costa Rica

GnRHa‐induced Multiple Spawns and Volition Spawning of Captive Spotted Rose Snapper, Lutjanus guttatus, at Mazatlan, Mexico

Effects of hypoxia and elevated ammonia concentration on the viability of red snapper embryos and early larvae

Contact Info

Product

Resources

About