Induced resistance in nectarine fruit by Bacillus licheniformis W10 for the control of brown rot caused by Monilinia fructicola

Ji, Zhao-Lin; Peng, Shaoyi; Zhu, Wei; Dong, Jingping; Feng, Zhaozhong

doi:10.1016/j.fm.2020.103558

Cited by 29 publications

(22 citation statements)

References 60 publications

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“…Surfactin and fengycin can also be elicitors of induced systemic resistance in plants ( Romero et al, 2007 ; Ongena et al, 2010 ). Many studies have reported that plant defense enzymes play important roles in disease resistance ( Prasannath, 2017 ; Ji et al, 2020 ; Xu et al, 2021 ). Induction of the activities of defense-related enzymes in leaves following soil inoculation with B. subtilis YB-04 and Foc indicates a form of systemic resistance.…”

Section: Discussionmentioning

confidence: 99%

Evaluation and Genome Analysis of Bacillus subtilis YB-04 as a Potential Biocontrol Agent Against Fusarium Wilt and Growth Promotion Agent of Cucumber

Yang

et al. 2022

Front. Microbiol.

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Cucumber wilt caused by Fusarium oxysporum f.sp. cucumerinum (Foc) is a highly destructive disease that leads to reduced yield in cucumbers. In this study, strain YB-04 was isolated from wheat straw and identified as Bacillus subtilis. It displayed strong antagonistic activity against F. oxysporum f.sp. cucumerinum in dual culture and exhibited significant biocontrol of cucumber Fusarium wilt with a higher control effect than those of previously reported Bacillus strains and displayed pronounced growth promotion of cucumber seedlings. B. subtilis YB-04 could secrete extracellular protease, amylase, cellulose, and β-1,3-glucanase and be able to produce siderophores and indole acetic acid. Inoculation with B. subtilis YB-04 or Foc increased cucumber defense-related enzyme activities for PPO, SOD, CAT, PAL, and LOX. However, the greatest increase was with the combination of B. subtilis YB-04 and Foc. Sequencing the genome of B. subtilis YB-04 showed that it had genes for the biosynthesis of various secondary metabolites, carbohydrate-active enzymes, and assimilation of nitrogen, phosphorous, and potassium. B. subtilis YB-04 appears to be a promising biological control agent against the Fusarium wilt of cucumber and promotes cucumber growth by genomic, physiological, and phenotypic analysis.

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Section: Discussionmentioning

confidence: 99%

Evaluation and Genome Analysis of Bacillus subtilis YB-04 as a Potential Biocontrol Agent Against Fusarium Wilt and Growth Promotion Agent of Cucumber

Yang

et al. 2022

Front. Microbiol.

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“…Most Bacillus spp. are known as the principal biological components used in various organic agriculture materials, owing to their stress management, disease prevention, and plant growth promotion properties [ 11 , 18 , 19 ]. The AMEM-producing company highlighted the presence of Bacillus species in their products, so as the first step of identification, all the isolates were grown on chromogenic HiCrome™ Bacillus agar, a differential media for Bacillus species [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

“…The bacterial isolates A-1, B-1, and C-1 were white, irregular, and wrinkle shaped with yellow/pinkish-colored media pigmentation ( Figure 2 ), which are the characteristic features of B. subtilis on HiCrome™ Bacillus agar medium [ 20 ]. However, B. amyloliquefaciens also displays similar culture characteristics [ 19 ]. These results provide the elementary evidence that isolates A-1, B-1, and C-1 may be B. subtilis , as supported by Alippi and Abrahamovich (2019) [ 20 ], who demonstrated the growth of 31 different strains of B. subtilis on HiCrome™ Bacillus agar and observed that 70.9% (22/31) of strains emerged as white, irregular, and wrinkle-shaped colonies with yellow-colored media pigmentation.…”

Section: Resultsmentioning

confidence: 99%

Study on the Identification Methods for Effective Microorganisms in Commercially Available Organic Agriculture Materials

et al. 2020

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The identification of microorganisms in closely related groups is challenging. The present work focused on the different molecular methodology for the accurate microbial identification in the five commercially available organic agriculture materials enriched with effective microorganisms. From the tested five organic agricultural materials, a total of seven distinct bacterial colonies (A-1, B-1, C-1, D-1, E-1, E-2, and E-3) were isolated and processed for sequential identification utilizing HiCrome™ Bacillus agar, biochemical tests with API CHB50, 16S rRNA gene analysis, random amplified polymorphic DNA (RAPD), and species-specific PCR analysis. All the isolated microorganisms were Gram-positive rods and spore former belonging to Bacillus group and appeared as a differential characteristic feature on HiCrome™ Bacillus agar. All isolates showed high-percentage similarities with the different members of Bacillus species in biochemical testing and 16S rRNA gene analysis. The collective identification results revealed isolates, A-1, B-1, and C-1, close to B. velezensis. Further RAPD-PCR and species-specific PCR discriminated and provided confirmatory evidence for D-1 as B. thuringiensis and E-1, E-2, and E-3 as B. licheniformis, respectively. In addition, presence of B. thuringiensis was also confirmed by toxin crystal protein staining. In conclusion, the species-specific primers could be used as a rapid and accurate identification tool to discriminate closely related Bacillus species such as B. subtilis, B. licheniformis, and B. thuringiensis.

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“…The mechanism by which Bacillus spp. control plant diseases includes the lysis of pathogenic fungus hyphae and the production of antifungal metabolites, plant growth promotion (PGP), production of antibiotics, nutrient and space competition, and induction of plant system resistance [ 49 ]. In this study, the CZ-6 strain produced several extracellular hydrolases, including cellulase, protease, and xylanase.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Salt-Tolerant Antagonistic Bacteria CZ-6 on the Rhizosphere Microbial Community of Winter Jujube (Ziziphus jujuba Mill. “Dongzao”) in Saline-Alkali Land

Zhou

Hao

et al. 2021

BioMed Research International

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As the main economic crop cultivated in the Yellow River Delta, winter jujube contains various nutrients. However, soil salinization and fungal diseases have affected the yield and quality of winter jujube. In order to use plant growth-promoting rhizobacteria (PGPR) to reduce these damages, the antagonistic bacteria CZ-6 isolated from the rhizosphere of wheat in saline soil was selected for experiment. Gene sequencing analysis identified CZ-6 as Bacillus amyloliquefaciens. In order to understand the salt tolerant and disease-resistant effects of CZ-6 strain, determination of related indicators of salt tolerance, pathogen antagonistic tests, and anti-fungal mechanism analyses was carried out. A pot experiment was conducted to evaluate the effect of CZ-6 inoculation on the rhizosphere microbial community of winter jujube. The salt tolerance test showed that CZ-6 strain can survive in a medium with a NaCl concentration of 10% and produces indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. Studies on the inhibition mechanism of pathogenic fungi show that CZ-6 can secrete cellulase, protease, and xylanase. Gas chromatography-mass spectrometry (GC-MS) analysis showed that CZ-6 can release volatile organic compounds (VOCs), including 2-heptanone and 2-nonanone. In addition, the strain can colonize the rhizosphere and migrate to the roots, stems, and leaves of winter jujube, which is essential for plant growth or defense against pathogens. Illumina MiSeq sequencing data indicated that, compared to the control, the abundance of salt-tolerant bacteria Tausonia in the CZ-6 strain treatment group was significantly increased, while the richness of Chaetomium and Gibberella pathogens was significantly reduced. Our research shows that CZ-6 has the potential as a biological control agent in saline soil. Plant damage and economic losses caused by pathogenic fungi and salt stress are expected to be alleviated by the addition of salt-tolerant antagonistic bacteria.

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Induced resistance in nectarine fruit by Bacillus licheniformis W10 for the control of brown rot caused by Monilinia fructicola

Cited by 29 publications

References 60 publications

Evaluation and Genome Analysis of Bacillus subtilis YB-04 as a Potential Biocontrol Agent Against Fusarium Wilt and Growth Promotion Agent of Cucumber

Evaluation and Genome Analysis of Bacillus subtilis YB-04 as a Potential Biocontrol Agent Against Fusarium Wilt and Growth Promotion Agent of Cucumber

Study on the Identification Methods for Effective Microorganisms in Commercially Available Organic Agriculture Materials

The Effect of Salt-Tolerant Antagonistic Bacteria CZ-6 on the Rhizosphere Microbial Community of Winter Jujube (Ziziphus jujuba Mill. “Dongzao”) in Saline-Alkali Land

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