Induced production of steroids by co-cultivation of two endophytes from Mahonia fortunei

Terpenoids From the Coral-Derived Fungus Trichoderma harzianum (XS-20090075) Induced by Chemical Epigenetic Manipulation

“…It has been revealed that fungi possess far more gene clusters encoding secondary metabolites than their characterized compounds (Khaldi et al, 2010). In order to solve this challenge, a number of manipulations have been used to regulate the production of secondary metabolites from fungi, such as One strain many compounds (OSMAC) (Pan et al, 2019), co-culture (Yu et al, 2019), interspecies crosstalk (Wang et al, 2019), and heterologous expression (Huo et al, 2019;Zhang et al, 2019). Among these methods, chemical epigenetic manipulation has been demonstrated to be a promising strategy to wake the silent biosynthetic gene clusters to obtain novel compounds and has been applied to the marine fungi (Asai et al, 2013).…”

Heptaketides from the endophytic fungusPleosporalessp. F46 and their antifungal and cytotoxic activities

“…Based on our previous results showing that several heptaketides displayed potent inhibitory activities against C. albicans, 9 the antifungal activities of the isolated metabolites (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) were investigated in the present study to further test this hypothesis. Only compound 1, which has a core structure similar to that of our previously reported compound biatriosporin D with high antifungal activity, exhibited weak antifungal activity against C. albicans with an MIC 80 of 128 mg mL À1 .…”

“…ECD calculations for compounds 2, 10, and 11 were performed as described previously. 7 The possible conformers were searched using Frog2 online version. 15 Further geometrical optimization and vibrational evaluation with Gaussian using DFT calculations with the B3PW91 functional and the TZVP basis set were carried out.…”

“…[1][2][3] They have been recognized as a valuable source of skeletally diverse and biologically active secondary metabolites and are attracting considerable attention from chemists and biologists. [4][5][6] As part of our ongoing search for fungal endophytes capable of producing biologically active compounds, 7 we recently isolated an endophytic fungus, Pleosporales sp. F46, from the medicinal plant Mahonia fortunei collected from Qingdao, People's Republic of China.…”

“…F46, from the medicinal plant Mahonia fortunei collected from Qingdao, People's Republic of China. 7 The OSMAC (One Strain Many Compounds) approach with the aid of LC-ESI-HRMS was employed to explore the chemical diversity of this endophyte. 8 When cultured in rice medium, Pleosporales sp.…”