Induced pluripotent stem cells from highly endangered species

Ben-Nun, Inbar Friedrich; Montague, Susanne C; Houck, Marlys L.; Tran, Ha Thi Thanh; Garitaonandia, Ibon; Leonardo, Trevor R; Wang, Yu-Chieh; Charter, Suellen J.; Laurent, Louise C.; Ryder, Oliver A.; Loring, Jeanne F.

doi:10.1038/nmeth.1706

Cited by 173 publications

(59 citation statements)

References 12 publications

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“…Recently, iPSC generations of endangered species were achieved in orangutan, drill, and northern white rhinoceros ( 27 , 28 ). Because all exhibited prime pluripotency, it seems difficult to contribute to host embryos as a chimera.…”

Section: Discussionmentioning

confidence: 99%

Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

et al. 2017

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“…In addition we have seen the translation of this technology to other arenas including the derivation of iPSCs from endangered species and farm animals [32]. Disease specific lines were first reported in 2008 by Park and colleagues, who describe 10 disease specific iPSC lines [12].…”

Section: Human Induced Pluripotent Stem Cells For Modelling Diseasementioning

confidence: 99%

Modelling Human Disease with Pluripotent Stem Cells

Siller¹,

Greenhough²,

Park³

et al. 2013

CGT

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Recent progress in the field of cellular reprogramming has opened up the doors to a new era of disease modelling, as pluripotent stem cells representing a myriad of genetic diseases can now be produced from patient tissue. These cells can be expanded and differentiated to produce a potentially limitless supply of the affected cell type, which can then be used as a tool to improve understanding of disease mechanisms and test therapeutic interventions. This process requires high levels of scrutiny and validation at every stage, but international standards for the characterisation of pluripotent cells and their progeny have yet to be established. Here we discuss the current state of the art with regard to modelling diseases affecting the ectodermal, mesodermal and endodermal lineages, focussing on studies which have demonstrated a disease phenotype in the tissue of interest. We also discuss the utility of pluripotent cell technology for the modelling of cancer and infectious disease. Finally, we spell out the technical and scientific challenges which must be addressed if the field is to deliver on its potential and produce improved patient outcomes in the clinic.

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“…ASCs can assist in chimpanzee conservation efforts by protecting their genetic diversity. The idea that cells can be used in this manner is becoming more widely recognized as the utility of “frozen zoos” is gaining credibility (Ben-Nun et al 2011). Recently, Ben-Nun et al (2011) created iPSC from two highly endangered species, the northern white rhinoceros and the drill monkey.…”

Section: Stem Cells Can Greatly Expand the Number Of Ex Vivo Models Fmentioning

confidence: 99%

“…The idea that cells can be used in this manner is becoming more widely recognized as the utility of “frozen zoos” is gaining credibility (Ben-Nun et al 2011). Recently, Ben-Nun et al (2011) created iPSC from two highly endangered species, the northern white rhinoceros and the drill monkey. The authors expressed the hope that these resources could “facilitate the reintroduction of genetic material into the population” in the future—a prospect that seems increasingly practical with the development of methods for reprogramming adult stem cells into haploid spermatogenic cells (Equizabal et al 2011; Easley et al 2012).…”

Section: Stem Cells Can Greatly Expand the Number Of Ex Vivo Models Fmentioning

confidence: 99%

Insights from a Chimpanzee Adipose Stromal Cell Population: Opportunities for Adult Stem Cells to Expand Primate Functional Genomics

Pfefferle

Wray

2013

Genome Biology and Evolution

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Comparisons between humans and chimpanzees are essential for understanding traits unique to each species. However, linking important phenotypic differences to underlying molecular changes is often challenging. The ability to generate, differentiate, and profile adult stem cells provides a powerful but underutilized opportunity to investigate the molecular basis for trait differences between species within specific cell types and in a controlled environment. Here, we characterize adipose stromal cells (ASCs) from Clint, the chimpanzee whose genome was first sequenced. Using imaging and RNA-Seq, we compare the chimpanzee ASCs with three comparable human cell lines. Consistent with previous studies on ASCs in humans, the chimpanzee cells have fibroblast-like morphology and express genes encoding components of the extracellular matrix at high levels. Differentially expressed genes are enriched for distinct functional classes between species: immunity and protein processing are higher in chimpanzees, whereas cell cycle and DNA processing are higher in humans. Although hesitant to draw definitive conclusions from these data given the limited sample size, we wish to stress the opportunities that adult stem cells offer for studying primate evolution. In particular, adult stem cells provide a powerful means to investigate the profound disease susceptibilities unique to humans and a promising tool for conservation efforts with nonhuman primates. By allowing for experimental perturbations in relevant cell types, adult stem cells promise to complement classic comparative primate genomics based on in vivo sampling.

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Induced pluripotent stem cells from highly endangered species

Cited by 173 publications

References 12 publications

Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

Flexible adaptation of male germ cells from female iPSCs of endangered Tokudaia osimensis

Modelling Human Disease with Pluripotent Stem Cells

Insights from a Chimpanzee Adipose Stromal Cell Population: Opportunities for Adult Stem Cells to Expand Primate Functional Genomics

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