Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinoceros

Zywitza, Vera; Frahm, Silke; Krüger, Norman; Weise, Anja; Göritz, Frank; Hermes, Robert; Holtze, Susanne; Colleoni, Silvia; Galli, Cesare; Drukker, Micha; Hildebrandt, Thomas B.; Diecke, Sebastian

doi:10.1016/j.isci.2022.105414

Cited by 8 publications

(5 citation statements)

References 31 publications

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“…While the reprogramming factors are highly conserved, the PESC maintenance medium ( Choi et al., 2019 ) was an important advance to allow the establishment of transgene-free PiPSCs in this study ( Figure 3 J). We anticipate that a diverse repertoire of iPSCs from various species, free of transgenes, can be established, although species-specific barriers may be encountered ( Dejosez et al., 2023 ; Korody et al., 2021 ; Yoshimatsu et al., 2021 ; Zywitza et al., 2022a , 2022b ). To fully unleash the potential to generate multilineage derivatives, we will need a better understanding of the various types of pluripotent states and a continued refinement of the species-specific culture conditions, optimized based on embryo-derived PSCs ( Choi et al., 2019 ; Gao et al., 2019 ; Yu et al., 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing

Conrad,

Meyer,

Ramesh

et al. 2023

Stem Cell Reports

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Section: Resultsmentioning

confidence: 99%

Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing

Conrad,

Meyer,

Ramesh

et al. 2023

Stem Cell Reports

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“…6 ENL-2 [49] Asian elephant (Elephas maximus) fibroblast lines 7 KCB 96008 [50] 8 KDF [51] Sumatran rhinoceros (Dicerorhinus sumatrensis) fibroblast lines 9 SR-fibroblasts [26] 10 Fish 80 [52] Rainbow trout (Oncorhynchus mykiss) tissues 11 pA03_wD06 [53] 12 UCLAi090-A [54] Rhesus macaque (Macaca mulatta) iPSC line…”

Section: Biosample Name Publication Characterizationmentioning

confidence: 99%

“…Further, the publication of animal cell lines in general and derived pluripotent animal stem cell lines in particular (i.e., embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs)) is constantly increasing. This applies to wildlife species threatened with extinction [ 21 , 22 , 23 , 24 , 25 , 26 ], as well as domesticated and livestock species [ 27 , 28 , 29 , 30 , 31 , 32 , 33 ] and model species, including non-human primates, mice, naked mole rats and others [ 34 , 35 , 36 , 37 , 38 , 39 ]. Also, the establishment of stem-cell-derived multicellular models such as organoids, assembloids and blastoids [ 40 , 41 ] is progressing for animal species, and they have been published both for model species, e.g., for mouse blastoids [ 42 ], and wildlife species, e.g., for rhinoceros cerebral organoids [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…This applies to wildlife species threatened with extinction [ 21 , 22 , 23 , 24 , 25 , 26 ], as well as domesticated and livestock species [ 27 , 28 , 29 , 30 , 31 , 32 , 33 ] and model species, including non-human primates, mice, naked mole rats and others [ 34 , 35 , 36 , 37 , 38 , 39 ]. Also, the establishment of stem-cell-derived multicellular models such as organoids, assembloids and blastoids [ 40 , 41 ] is progressing for animal species, and they have been published both for model species, e.g., for mouse blastoids [ 42 ], and wildlife species, e.g., for rhinoceros cerebral organoids [ 26 ]. Research on the cellular material of domesticated model species has been conducted intensively in the last decades, with the result being more than 174,000 mouse ( Mus musculus ) ES cell lines having been established, registered by the RII and assigned an RRID so far [ 43 ] and more than 2 million mouse biosamples having been registered in the BioSamples database.…”

Section: Introductionmentioning

confidence: 99%

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A Standardized Nomenclature Design for Systematic Referencing and Identification of Animal Cellular Material

Schrade,

Mah,

Bandrowski

et al. 2024

Animals

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The documentation, preservation and rescue of biological diversity increasingly uses living biological samples. Persistent associations between species, biosamples, such as tissues and cell lines, and the accompanying data are indispensable for using, exchanging and benefiting from these valuable materials. Explicit authentication of such biosamples by assigning unique and robust identifiers is therefore required to allow for unambiguous referencing, avoid identification conflicts and maintain reproducibility in research. A predefined nomenclature based on uniform rules would facilitate this process. However, such a nomenclature is currently lacking for animal biological material. We here present a first, standardized, human-readable nomenclature design, which is sufficient to generate unique and stable identifying names for animal cellular material with a focus on wildlife species. A species-specific human- and machine-readable syntax is included in the proposed standard naming scheme, allowing for the traceability of donated material and cultured cells, as well as data FAIRification. Only when it is consistently applied in the public domain, as publications and inter-institutional samples and data are exchanged, distributed and stored centrally, can the risks of misidentification and loss of traceability be mitigated. This innovative globally applicable identification system provides a standard for a sustainable structure for the long-term storage of animal bio-samples in cryobanks and hence facilitates current as well as future species conservation and biomedical research.

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“…These applications range through various biomedical disciplines, including development of cell therapies, disease modeling and drug testing, and clinical application for untreatable diseases in both people and companion animals ( 35 , 122 , 123 ). In addition, as a characteristic application in animal species and important for the maintenance of biodiversity, iPSCs have been generated from critically endangered mammalian ( 32 , 103 , 124 , 125 ) and avian species ( 126 ). These are important for the study of developmental and physiological species-specific processes, and as wildlife preservation efforts they are important for the conservation of genetic resources and maintenance of a healthy eco-system.…”

Section: Applications Of Ipscs In Companion Animalsmentioning

confidence: 99%

Induced pluripotent stem cells in companion animals: how can we move the field forward?

et al. 2023

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Following a one medicine approach, the development of regenerative therapies for human patients leads to innovative treatments for animals, while pre-clinical studies on animals provide knowledge to advance human medicine. Among many different biological products under investigation, stem cells are among the most prominent. Mesenchymal stromal cells (MSCs) are extensively investigated, but they present challenges such as senescence and limited differentiation ability. Embryonic stem cells (ESCs) are pluripotent cells with a virtually unlimited capacity for self-renewal and differentiation, but the use of embryos carries ethical concerns. Induced pluripotent stem cells (iPSCs) can overcome all of these limitations, as they closely resemble ESCs but are derived from adult cells by reprogramming in the laboratory using pluripotency-associated transcription factors. iPSCs hold great potential for applications in therapy, disease modeling, drug screening, and even species preservation strategies. However, iPSC technology is less developed in veterinary species compared to human. This review attempts to address the specific challenges associated with generating and applying iPSCs from companion animals. Firstly, we discuss strategies for the preparation of iPSCs in veterinary species and secondly, we address the potential for different applications of iPSCs in companion animals. Our aim is to provide an overview on the state of the art of iPSCs in companion animals, focusing on equine, canine, and feline species, as well as to identify which aspects need further optimization and, where possible, to provide guidance on future advancements. Following a “step-by-step” approach, we cover the generation of iPSCs in companion animals from the selection of somatic cells and the reprogramming strategies, to the expansion and characterization of iPSCs. Subsequently, we revise the current applications of iPSCs in companion animals, identify the main hurdles, and propose future paths to move the field forward. Transferring the knowledge gained from human iPSCs can increase our understanding in the biology of pluripotent cells in animals, but it is critical to further investigate the differences among species to develop specific approaches for animal iPSCs. This is key for significantly advancing iPSC application in veterinary medicine, which at the same time will also allow gaining pre-clinical knowledge transferable to human medicine.

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Induced pluripotent stem cells and cerebral organoids from the critically endangered Sumatran rhinoceros

Cited by 8 publications

References 31 publications

Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing

Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing

A Standardized Nomenclature Design for Systematic Referencing and Identification of Animal Cellular Material

Induced pluripotent stem cells in companion animals: how can we move the field forward?

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