Induced Ovulation in Obscure Puffer Takifugu obscurus by Injections of LHRH-a

Dose-dependent effect of a single GnRHa injection on the spawning of meagre (Argyrosomus regius) broodstock reared in captivity

“…The observation in the present study that meagre have a narrow range of optimal dose of a single injection to induced spawning could be interpreted to be contradictory to studies that found a broad range of GnRHa doses successfully induced spawning in other species and that have concluded that for economic reasons lower doses should be used (Yang & Chen, 2004;Podhorec et al, 2012). However, the present study can also be interpreted to have found a broad range of doses (10-30) that have successfully induced spawning.…”

“…In the present study, reducing the dose from the optimal dose (15 µg kg -1 ) to lower than optimal doses (0, 1, 5 and 10 µg kg -1 ) exhibited a gradual increase in latency period and decrease in fecundity and egg quality, fertilisation, percentage of viable eggs and percentage hatch. Studies on other species have also found that lower than optimal doses resulted in longer latency period (Yang & Chen, 2004;Wang et al, 2009), decreased fecundity (Garcia, 1989;Barbaro et al, 1997;Ibarra & Duncan, 2007) and decreased percentage hatch (Ibarra & Duncan, 2007). In the present study, increasing doses to higher than optimal doses (20, 30, 40 and 50 µg kg -1 ) resulted in a gradual reduction in latency period, fecundity and egg quality, fertilisation, percentage of viable eggs and percentage hatch.…”

Ovarian maturation and histological observations of Barilius bendelisis (Hamilton) in captivity

“…It may be ascribed to the failure of completion of vitellogenesis and oocyte maturation of few developing oocytes. Similar results of reproductive dysfunction were observed in several captive-reared females (Mylonas and Zohar, 2001;Lee and Yang, 2002;Yang and Chen, 2004;Agullerio et al, 2006). According to Mylonas et al(2010) the failure of completion of vitellogenesis and oocyte maturation is due to the dysfunctional endocrine release of LH, which may be related to various environmental as well as social factors.…”

The effect of feeding frequency, water temperature, and stocking density on the growth of river puffer Takifugu obscurus reared in a zero-exchange water system

“…River puffer moves from sea to river for reproduction during the spawning season of May to June in Korea. Therefore, it has typical anadromous life history (Yang and Chen 2004). River puffer is one of the promising cultured fish species in South Korea because of its desirable taste and high market price (Ministry of Oceans and Fisheries 2013).…”

“…Previous investigations on the river puffer, however, have focused mainly on the development of larvae and juvenile, reproductive physiology, and seed production (Jang et al 1996;Park et al 1997;Son et al 2001;Yang and Chen 2004;Kato et al 2005;Yang and Chen 2006). We confirmed that BFT tank (217 %) showed a higher weight gain than the running seawater tank (164 %) in a preliminary study where 5-g-size river puffers were carried out in BFT tank and running seawater tank for 8 weeks (unpublished data).…”