Induced Ovulation and Artificial Insemination in a Rhesus Colony

Dede, J. Anthony; Plentl, Albert A.

doi:10.1016/s0015-0282(16)36125-8

Cited by 15 publications

(12 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From a practical standpoint of breeding, however, we think of several countermeasures that should be taken when laboratory-bred monkeys are defective in their mating and maternal behaviors. For a female monkey who can not 'copulate, artificial insemination is worth trying although its efficiency seems still low (Dede and Plentl, 1966;Valerio, Leverage and Munster, 1971;Peng, Lai and Yang, 1973;Settlage, Swan and Hendrickx, 1973;Cho, Honjo and Makita, 1975). For the baby monkey having been borne by a female who is defective in nursing behaviors, the first countermeasure is artificial nursing ) and the second is foster nursing (Cho and Honjo, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Breeding of Cynomolgus Monkeys Through Successive Generations by Indoor Cage System

Honjo

Cho

Fujiwara

et al. 1978

JJMSB

View full text Add to dashboard Cite

SUMMARY:Vital statistics on the breeding through successive generations were presented for the cynomolgus monkey colony of NIH, Tokyo. The results of this retrospective survey clearly demonstrated the third (F2) and the fourth (Fs) generations could be bred and reared successfully by the indoor caged-breeding system in which either individual timed-mating or group mating procedure was adopted. Several important and difficult problems involved in the production of successive generations of the cynomolgus monkey by our breeding system were discussed from the standpoint of laboratory animal science.

show abstract

Section: Discussionmentioning

confidence: 99%

Breeding of Cynomolgus Monkeys Through Successive Generations by Indoor Cage System

Honjo

Cho

Fujiwara

et al. 1978

JJMSB

View full text Add to dashboard Cite

show abstract

“…Dede and Plentl (1966) succeeded in getting pregnant rhesus macaques by means of artificial insemination.…”

Section: Spermatozoal Motilitymentioning

confidence: 99%

A Simplified Method for Collecting and Preserving Cynomolgus Macaque Semen

Cho

Honjo

1973

JJMSB

View full text Add to dashboard Cite

SUMMARY:Simplified and practical procedures for collecting macaque semen (containing little coagulum and for preserving semen in vitro for a long period were studied with cynomolgus monkeys that had proved fertile.The semen ejaculated into the vagina of the female mated was taken by aspirating with a pippet. A greater part of the semen taken in this way was usually represented by fluid.The semen thus obtained was diluted with a glycerolated extender containing lactose, glucose, raffinose and egg-yolk. The most adequate concentration of glycerol in the extender was 5%.Then, about 0.1 ml of diluted semen was frozen into a pellet form on a dry-ice block after about 30-min pre-cooling to 4C . The frozen pellet of semen was preserved in a liquid nitrogen container . The quality of the preserved semen was evaluated by its spermatozoal survival rate and motility; the semen has successfully been preserved for at least 20 weeks until the time of preparing this paper, maintaining about a 60% level of the spermatozoal survival rate.

show abstract

“…Artificial insemination (AI), which has been successful in the propagation of other mammalian species, including humans, could be most useful to increase the number of rhesus macaques available for biomedical research. Nevertheless, AI in the rhesus macaque is somewhat difficult, with pregnancy rates using fresh sperm ranging between 4% to 40% [4][5][6] and only one pregnancy reported after AI with cryopreserved sperm, in which the embryo was aborted at an early stage [7]. In the rhesus macaque, AI is limited by features of the female reproductive tract and by the viability of cryopreserved sperm.…”

Section: Introductionmentioning

confidence: 99%

Live Rhesus Offspring by Artificial Insemination Using Fresh Sperm and Cryopreserved Sperm1

Sanchez-Partida

Maginnis

Dominko

et al. 2000

Biology of Reproduction

View full text Add to dashboard Cite

Artificial insemination (AI) and the cryopreservation of sperm with full reproductive capabilities are vital in the armamentarium of infertility clinics and reproductive laboratories. Notwithstanding the fantastic successes with AI and sperm cryopreservation in numerous species, including humans and cattle, these assisted reproductive technologies are less well developed in other species of importance for biomedical research, such as genetically modified mice and nonhuman primates. To that end, AI at high efficiency in the rhesus macaque (Macaca mullata) and the successful cryopreservation of rhesus sperm is presented here, as are the complexities of this primate model due to differences in reproductive tract anatomy and gamete physiology. Cryopreservation had no effect on the ability of sperm to fertilize oocytes in vitro or in vivo. Post-thaw progressive motility was not affected by cryopreservation; however, acrosome integrity was lower for cryopreserved (74.1%) than for fresh sperm (92.7%). Fertilization rates did not differ when fresh (58.1%; n = 32/55) or cryopreserved sperm (63.8%; n = 23/36) were used for in vitro fertilization. Similarly, pregnancy rates did not differ significantly after AI with fresh (57.1%; n = 8/14) or cryopreserved sperm (62.5%; n = 5/8). Seven live rhesus macaques were born following AI with fresh sperm, and three live offspring and two ongoing pregnancies were obtained when cryopreserved sperm were used. Cryopreservation of rhesus sperm as presented here would allow for the cost-effective storage of lineages of nonhuman primates with known genotypes. These results suggest that either national or international centers could be established as repositories to fill the global needs of sperm for nonhuman primate research and to provide the experimental foundation on which to explore and perfect the preservation of sperm from endangered nonhuman primates.

show abstract

Induced Ovulation and Artificial Insemination in a Rhesus Colony

Cited by 15 publications

References 6 publications

Breeding of Cynomolgus Monkeys Through Successive Generations by Indoor Cage System

Breeding of Cynomolgus Monkeys Through Successive Generations by Indoor Cage System

A Simplified Method for Collecting and Preserving Cynomolgus Macaque Semen

Live Rhesus Offspring by Artificial Insemination Using Fresh Sperm and Cryopreserved Sperm1

Contact Info

Product

Resources

About