Induced endothelial differentiation of cells from a murine embryonic mesenchymal cell line C3H/10T1/2 by angiogenic factors in vitro

Wang, Mingke; Su, Yongping; Sun, Han; Wang, Tao; Gao, Yan; Ran, Xinze; Wang, Fengchao; Cheng, Tao; Zou, Zhongmin

doi:10.1016/j.diff.2009.08.002

Cited by 27 publications

(17 citation statements)

References 39 publications

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“…Enhanced migration of mesenchymal stem cells (MSCs) mediated by bFGF (Langer et al , ) or G‐CSF (Iohara et al , , ; Yu et al , ; Ponte et al , ; Murakami et al , ) has already been demonstrated separately in distinct cell types. The high stimulatory effect on proliferation and differentiation of MSCs into endothelial cells has been elucidated in bFGF‐supplemented medium (Wu et al , ; Wang et al , ) and in G‐CSF‐supplemented medium. The enhanced roles of bFGF and G‐CSF on neurogenesis and axonal growth in vitro have also been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte‐colony stimulating factor

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte‐colony stimulating factor

et al. 2014

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“…VEGF addition successfully induces the differentiation of endothelium from mouse EBs (Vittet et al, 1996), human ESCs (Nourse et al, 2010) and human breast tumour stem cells (Bussolati et al, 2009). VEGF and bFGF treatments induce endothelial differentiation of mouse MSCs (Wang et al, 2010). However, in our study, we did not add VEGF in EBs' culture.…”

Section: Discussionmentioning

confidence: 99%

Hypoxic priming of mESCs accelerates vascular‐lineage differentiation through HIF1‐mediated inverse regulation of Oct4 and VEGF

et al. 2012

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Hypoxic microenvironment plays an important role in determining stem cell fates. However, it is controversial to which direction between self-renewal and differentiation the hypoxia drives the stem cells. Here, we investigated whether a short exposure to hypoxia (termed ‘hypoxic-priming’) efficiently directed and promoted mouse embryonic stem cells (mESCs) to differentiate into vascular-lineage. During spontaneous differentiation of embryoid bodies (EBs), hypoxic region was observed inside EB spheroids even under normoxic conditions. Indeed, hypoxia-primed EBs more efficiently differentiated into cells of vascular-lineage, than normoxic EBs did. We found that hypoxia suppressed Oct4 expression via direct binding of HIF-1 to reverse hypoxia-responsive elements (rHREs) in the Oct4 promoter. Furthermore, vascular endothelial growth factor (VEGF) was highly upregulated in hypoxia-primed EBs, which differentiated towards endothelial cells in the absence of exogenous VEGF. Interestingly, this differentiation was abolished by the HIF-1 or VEGF blocking. In vivo transplantation of hypoxia-primed EBs into mice ischemic limb elicited enhanced vessel differentiation. Collectively, our findings identify that hypoxia enhanced ESC differentiation by HIF-1-mediated inverse regulation of Oct4 and VEGF, which is a novel pathway to promote vascular-lineage differentiation.

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“…Thus, it is important that an appropriate, nonimmunogenic protein and cell types be used for each device. Although generally safe recombinant human extracellular matrix proteins are readily available, recent advances in stem cell technology make true implantation safety a reasonable challenge to overcome; mesenchymal stem cells can be isolated from an individual patient's bone marrow, differentiated to endothelial cells in vitro , then vascularized on the device, as described here. Alternatively, additional surgery can be avoided by isolating fibroblasts from a patient skin sample, which can then be used to generate induced pluripotent stem cells .…”

Section: Discussionmentioning

confidence: 99%

Prevascularized silicon membranes for the enhancement of transport to implanted medical devices

et al. 2015

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Recent advances in drug delivery and sensing devices for in situ applications are limited by the diffusion-limiting foreign body response of fibrous encapsulation. In this study, we fabricated pre-vascularized synthetic device ports to help mitigate this limitation. Membranes with rectilinear arrays of square pores with widths ranging from 40 to 200 μm were created using materials (50 μm thick double-sided polished silicon) and processes (photolithography and directed reactive ion etching) common in the manufacturing of microfabricated sensors. Vascular endothelial cells responded to membrane geometry by either forming vascular tubes that extended through the pore or completely filling membrane pores after four days in culture. Although tube formation began to predominate overgrowth around 75 μm and continued to increase at even larger pore sizes, tubes formed at these large pore sizes were not completely round and had relatively thin walls. Thus, the optimum range of pore size for pre-vascularization of these membranes was estimated to be 75 - 100 μm. This study lays the foundation for creating a pre-vascularized port that can be used to reduce fibrous encapsulation and thus enhance diffusion to implanted medical devices and sensors.

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Induced endothelial differentiation of cells from a murine embryonic mesenchymal cell line C3H/10T1/2 by angiogenic factors in vitro

Cited by 27 publications

References 39 publications

Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte‐colony stimulating factor

Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte‐colony stimulating factor

Hypoxic priming of mESCs accelerates vascular‐lineage differentiation through HIF1‐mediated inverse regulation of Oct4 and VEGF

Prevascularized silicon membranes for the enhancement of transport to implanted medical devices

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