Induced cell death and its role in hemopoietic hypoplasia after cytostatic treatment

Abstract

The morphological signs of apoptosis in the bone-marrow cells and thymocytes, indices of cellularity in these organs and in peripheral blood and the absolute number of committed bone marrow cells-precursors have been studied on CBA mice injected with Etoposid (1/2 LD~0). The results of the study suggest that reduced cellular counts observed in the hemopoietic organs 3-6 h after the cytostatic injection are due to Etoposid-induced apoptosis.

Reaction of the blood system in pregnant rats at late terms after etoposide treatment

“…It cannot be excluded that the observed effect results from decreased lymphocyte count, because T cells and their lymphokines are known to regulate erythropoiesis [14,15]. The pronounced monocytic reaction is also typical of etoposide [4]. The observed changes in the mean parameters of PB cells were not accompanied by suppression of the erythroid hemopoietic stem in the bone marrow, but granulocytopoiesis was activated due to the increase in the number of immature neutrophil granulocytes (Fig.…”

“…The pattern of changes in PB (decreased erythrocyte count, hemoglobin content, hematocrit) and bone marrow (hyperplasia of the erythroid hemopoietic stem) as well as the mechanism underlying the effect of etoposide on the erythron [4] drove us to a conclusion on hemolytic nature of the observed anemia. The pronounced increase in erythropoiesis regeneration capacity 3 months after etoposide treatment was a compensatory reaction to superinducing influence of the cytostatic and after 6 months we observed a decrease in the number of erythroid elements and inhibition of their proliferation in the bone marrow.…”

“…1). This attests to recovery of bone marrow hemopoiesis suppressed at early terms after etoposide treatment [4].…”

“…These changes are typical of strained erythropoiesis. It is obvious, that these processes are determined by high sensitivity of the erythroid hemopoietic stem to etoposide [4]. It cannot be excluded that the observed effect results from decreased lymphocyte count, because T cells and their lymphokines are known to regulate erythropoiesis [14,15].…”

Pathomorphological Study of the Cytotoxic Effects of Cyclic Polychemotherapy on Reproductive Organs of Female Rats

“…Introduction of new antitumor agents and polychemotherapy (PCT) cycles in practical oncology improves the treatment of malignant tumors, leading to better results and appreciable prolongation of patient's lifespan. Modern antitumor therapy is characterized by high aggressiveness and toxicity influencing not only tumor, but also normal tissues [1,3], and causing structural and functional disorders in the reproductive organs [2,4,6].…”