Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

Arakawa, Tsutomu; Tsumoto, Kouhei; Ejima, Daisuke; Kita, Yoshiko; Yonezawa, Yasushi; Tokunaga, Masao

doi:10.1016/j.jbbm.2006.12.001

Cited by 15 publications

(9 citation statements)

References 25 publications

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“…4. Arakawa et al (25) had similar results, since the low ammonium sulfate concentration was the most successful condition to elute BSA, considering its molarities under evaluation.…”

Section: Resultsmentioning

confidence: 74%

Single Chromatographic Step for β-Galactosidase Purification: Influence of Salt and Elution Parameters

Braga

Lemes

Kalil

2014

Separation Science and Technology

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β-galactosidase purification was performed by ion exchange chromatography in which the main elution parameters and different elution salts (NaCl, KCl, and (NH 4 ) 2 SO 4 ) were evaluated through distinct purification strategies. Among the salts under analysis, KCl was the best one for β-galactosidase purification, since it reached a 95.1% recovery and an 8.2-fold purification factor. NaCl, which is the most common elution salt found in the literature, obtained a 75.1% recovery and a 7.5-fold purification factor whereas ammonium sulfate, as salt elution, yielded a 72.1% recovery and a 3-fold purification factor in the same conditions.

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“…4. Arakawa et al (25) had similar results, since the low ammonium sulfate concentration was the most successful condition to elute BSA, considering its molarities under evaluation.…”

Section: Resultsmentioning

confidence: 74%

Single Chromatographic Step for β-Galactosidase Purification: Influence of Salt and Elution Parameters

Braga

Lemes

Kalil

2014

Separation Science and Technology

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“…The supernatant was diluted into 2 M ammonium sulfate containing binding buffer for a modified Blue-Sepharose chromatography, described previously (23) and eluted using 2 M NaCl (Eluate 1) and 1 M arginine (Eluate 2). The eluted fractions were concentrated and dialyzed against phosphate-buffered saline (PBS) using Vivaspin 6 columns (GE Healthcare) and then tested for induction of TEAD activity using luciferase assay (described below).…”

Section: Methodsmentioning

confidence: 99%

Serum Inter-α-inhibitor Activates the Yes Tyrosine Kinase and YAP/TEAD Transcriptional Complex in Mouse Embryonic Stem Cells

Pijuan-Galito

Tamm

Annerén

2014

Journal of Biological Chemistry

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Background: Serum contributes to embryonic stem (ES) cell maintenance of pluripotency and activates Yes. Results: The serum protein Inter-␣-inhibitor (I␣I) was found to activate the Yes/YAP/TEAD transcription factor pathway and induce expression of Oct3/4 and Nanog in ES cells. Conclusion: I␣I activates key pluripotency pathways. Significance: I␣I may play a significant role in ES cell maintenance and self-renewal.

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“…All these results are consistent with hydrophobic binding to the highly hydrophilic resin in the presence of salting‐out salts. In fact, ammonium sulfate even produces binding of proteins to highly polar ion exchange columns, which simply reflects its strong salting‐out property 49, 50…”

Section: Solvent Effectsmentioning

confidence: 99%

The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals

Arakawa

Ejima

et al. 2010

Journal of Pharmaceutical Sciences

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198

118

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Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

Cited by 15 publications

References 25 publications

Single Chromatographic Step for β-Galactosidase Purification: Influence of Salt and Elution Parameters

Single Chromatographic Step for β-Galactosidase Purification: Influence of Salt and Elution Parameters

Serum Inter-α-inhibitor Activates the Yes Tyrosine Kinase and YAP/TEAD Transcriptional Complex in Mouse Embryonic Stem Cells

The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals

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