Transportation research projects are a vital element for transportation agencies to accumulate new technology and knowledge to improve the efficiency and safety of transportation infrastructure. The purpose of this paper is to develop benefit cost analysis (BCA) tools to assist transportation agencies in evaluating transportation research projects. The BCA tools provide analysis methods for estimating the benefits for changes in the level of service (LOS) of a roadway, reductions in the vehicle travel time, changes in vehicle operating costs and reductions in the number of crashes. Three case studies were investigated to illustrate the usage of the BCA tools. The first case study evaluated the effectiveness of variable speed limit systems implemented along I-80 in southern Wyoming. The BCA tools estimated a benefit cost ratio of 6.64 indicating that the implementation of the project was economically justifiable. The second case study was a recent study investigating and developing mitigation strategies for the effects of truck traffic along the I-80 in southern Wyoming resulting in a benefit cost ratio of a 9.23 indicating that the project had the potential to be economically viable upon implementation. The final case study evaluated the effect of wildlife overpass and underpass crossings on the migrations of pronghorn and mule deer. The benefit cost ratio for the research project was 0.42 indicating that the project was not economically beneficial after the first year of implementation. The case studies indicated that the BCA tools are a beneficial tool for quantifying the economic benefits of a transportation research project.